Abstract
Aims/hypothesis
Ion fluxes constitute a major integrative signal in beta cells that leads to insulin secretion and regulation of gene expression. Understanding these electrical signals is important for deciphering the endogenous algorithms used by islets to attain homeostasis and for the design of new sensors for monitoring beta cell function.
Methods
Mouse and human islets were cultured on multielectrode arrays (MEAs) for 3–13 days. Extracellular electrical activities received on each electrode were continuously amplified and recorded for offline characterisation.
Results
Differential band-pass filtering of MEA recordings of mouse islets showed two extracellular voltage waveforms: action potentials (lasting 40–60 ms) and very robust slow potentials (SPs, lasting 800–1,500 ms), the latter of which have not been described previously. The frequency of SPs directly correlated with glucose concentration, peaked at 10 mmol/l glucose and was further augmented by picomolar concentrations of glucagon-like peptide-1. SPs required the closure of ATP-dependent potassium channels as they were induced by glucose or glibenclamide but were not elicited by KCl-induced depolarisation. Pharmacological tools and the use of beta cell specific knockout mice showed that SPs reflected cell coupling via connexin 36. Moreover, increasing and decreasing glucose ramps showed hysteresis with reduced glucose sensitivity during the decreasing phase. SPs were also observed in human islets and could be continuously recorded over 24 h.
Conclusions/interpretation
This novel electrical signature reflects the syncytial function of the islets and is specific to beta cells. Moreover, the observed hysteresis provides evidence for an endogenous algorithm naturally present in islets to protect against hypoglycaemia.
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Introduction
Islets play a central role in nutrient homeostasis acting as sensors and storage as well as release sites for the principal hormones involved in glucose regulation [1]. Beta cells are activated by glucose, the metabolism of which induces a change in coupling factors, including an increase in the ratio of ATP/ADP leading to the closure of ATP-dependent potassium (KATP) channels. The ensuing membrane depolarisation opens voltage-dependent channels [1] and the resulting calcium influx regulates insulin exocytosis as well as gene expression. Beta cells are an ideal sensor for insulin demand since they are also sensitive to hormones that relay the status of the organism. Incretins, such as glucagon-like peptide-1 (GLP-1), act via G-protein coupled receptors to potentiate the electrical response and secretion of insulin [2]. By contrast, stress hormones like adrenaline (epinephrine) reduce electrical activity and hormone exocytosis during physical activity [3]. Therefore, changes in cell membrane potential represent an integrative signal read-out that encodes the body’s demand for insulin.
Glucose-induced metabolism-secretion coupling has been described in great detail and is characterised by oscillations of metabolites, second messengers, membrane potential and insulin secretion, with a frequency ranging from one event per 10 s to one per 1 min or per several min in the case of insulin secretion [4]. Oscillations may be part of endogenous algorithms that beta cells use for nutrient homeostasis. Moreover, they are disrupted under pathological conditions, such as lipotoxicity [5] or type 2 diabetes [6]. Investigating integrative signals, such as electrical activity, is required for deciphering endogenous algorithms used by islets to attain homeostasis; it may help to improve algorithms in continuous glucose monitoring and to develop novel sensors [7, 8]. Standard electrophysiology approaches to determine glucose-dependent signal frequencies in islets require special configurations, such as sharp microelectrodes or perforated patches. These techniques are time-consuming and restricted to the observation of individual cells for a limited time. By contrast, extracellular recordings on multielectrode arrays (MEAs) offer a novel approach for physiological experiments with intact and native islet cells in a multicellular context [7, 9, 10].
Using MEAs, we found a novel electrical signature of islet cells: slow potentials (SPs). SPs reflect the syncytial behaviour of the islets, play an important role in glucose homeostasis and are a useful biomarker of cell coupling and islet function. Our findings suggest that insulin demand may be encoded by the overall frequency of SPs rather than by the bursting behaviour of islets.
Methods
Chemicals
All chemicals were obtained from Sigma-Aldrich (St. Louis, MO, USA) except for GLP-1 (Bachem Bioscience, King of Prussia, PA, USA). Nifedipine and glibenclamide were solubilised in dimethyl sulfoxide, and adrenaline was solubilised in ascorbic acid. Solvents (final concentrations ≤0.1%, vol./vol.) were without effect on SPs.
Animals
Adult (8–80 week old) C57BL/6 mice were used as the wild-type (WT) animals (obtained from Bordeaux University Animal House breeding, Bordeaux, France). Age-matched conditional mice floxed (Flox: Cx36 Flox(CFP)/Flox(CFP)) or deleted specifically in beta cells (RIP-Cre: Cx36 Flox(CFP)/Flox(CFP):RIP-Cre) for Cx36 (also known as Gjd2) were from the Geneva colony [11]. All experimental procedures were approved by the French Ministry of Education and Research (No.01836.01).
Islet isolation and cell culture
We obtained 100–200 islets per mouse and these were cultured for 2–7 days on MEAs (MEA100/10-Ti-gr and MEA200/30iR-Ti-gr, Multichannel Systems, Reuttlingen, Germany) as whole islets or partially dissociated islet cell clusters (>10 cells per cluster), as previously described [12]. The use of human islets was approved by the ethics committee of Geneva University Hospital (Geneva, Switzerland). Human islets were isolated from three cadaveric non-diabetic donors (see electronic supplementary material [ESM] Table 1) at the Cell Isolation and Transplantation Center (Geneva University Hospital) [13]. After transport, the human islets were maintained in suspension for recovery (25°C, 95% O2, >90% relative humidity) in CMRL-1066 medium (Invitrogen, Carlsbad, CA, USA) containing 5.6 mmol/l glucose, supplemented with FBS (10% vol./vol.; Eurobio, Courtaboeuf, France), penicillin-streptomycin (1% vol./vol.; Invitrogen, Saint Aubin, France) and l-glutamine (1% vol./vol.; Invitrogen). The islets were subsequently cultured on MEAs in this medium for 5–13 days using the same procedure as for mouse islets. Culture media were renewed every 2–3 days.
Electrophysiology and signal analysis
Extracellular recordings were performed at 37°C as previously described [12] in a buffer containing NaCl 135 mmol/l, KCl 4.8 mmol/l, MgCl2 1.2 mmol/l, CaCl2 1.2–1.8 mmol/l, HEPES 10 mmol/l and glucose as indicated (pH 7.4 adjusted with NaOH). Simultaneous analogue data were acquired at 10 kHz for all electrodes using a MEA1060-Inv-BC-Standard amplifier (Multichannel Systems; gain: 1,100; analogue filter: changed from default 10–3,000 to 0.1–3,000 Hz) and treated with MC_Rack software (Multichannel Systems, Tübingen, Germany). A 0.2–700 Hz second-order Butterworth digital filter provided by MC_Rack software (www.multichannelsystems.com/software/mc-rack) was used for representative traces (except in Fig. 1e and f). For offline determination of frequencies, SPs were extracted using a 0.2–2 Hz band-pass filter (see Fig. 1f), and detected using the threshold module of MC_Rack with a dead time (minimal period between two events) set to 500 ms. SP frequencies were measured at steady state during the last 3–7 min of each condition.
For long-term recordings, MEAs seeded with human islet cells were connected to the amplifier and placed in a dry incubator (95% O2) at 37°C. Recordings were performed without perfusion in culture medium with 7 mmol/l glucose. The MEA was covered with a fluorinated ethylene-propylene membrane (ALA Scientific Instruments, Farmingdale, NY, USA) for gas exchange, with evaporation limited to 0.19 ± 0.03% of the total volume per h (seven measures) [14]. Signals were continuously acquired at 2 kHz and SP frequencies were determined offline using the procedure detailed above for successive periods of 10 min over 24 h.
The correlation in Fig. 1g was obtained with MATLAB software (MathWorks, Cambridge, UK). The relative angular position of 4,400 action potentials (APs) on their respective SPs was determined for four independent experiments in mouse islets recorded at 3–11 mmol/l glucose. SPs were extracted by determining their positive and negative extremes, APs were isolated using the wavelet transforms method [10]. The angular position of each AP was then computed between 0° and 180° (rising phase of SPs) or between 180° and 360° (falling phase).
Statistics
Experiments were replicated on at least three MEAs with cells from different mice or donors. Results are presented as mean values with SEM for N electrodes. Student’s t test was used for paired data. ANOVA with Bonferroni’s or Student–Newman–Keuls correction as a post hoc test was used for comparisons between two or more groups.
Results
The electrical activity of islet cells seeded on MEAs (Fig. 1a) was recorded as illustrated in Fig. 1b for one electrode. The electrical activity of mouse islets increased when glucose concentration was raised from 3 mmol/l to 15 mmol/l (Fig. 1c). A higher temporal resolution of this glucose-induced activity (Fig. 1d) revealed the presence of two distinct and superimposed signals: rapid spikes of small amplitude (40–60 ms in duration, 10–50 μV), which have previously been characterised as APs [10]. In addition, we found slower oscillations of larger amplitude (800–1,500 ms period, 50–350 μV peak-to-peak), we refer to as SPs. Differential band-pass filtering separated the two signals (Fig. 1e, f). While filtering at 2–700 Hz sorted the short-lasting APs, filtering at 0.2–2 Hz separated-out the SPs and was used for further quantification of SP frequencies. Probably due to their small amplitude, APs were not visible in 32.2 ± 3.7% of electrodes with SPs (n=20 experiments). When present, APs were downward on the majority of recordings but upward in a few cases (96% vs 4%, respectively) as in our previous report [10]. Temporal correlations between the two signals showed that APs mainly occurred during the falling (69%) rather than the rising phase (31%) of SPs (Fig. 1g). Both signals were observed without difference in entire islets as well as islet cell clusters, and were recorded as early as after 6 h in culture (not shown). Of note, SP amplitudes decreased with increasing concentration of extracellular calcium and were extinguished above 2.5 mmol/l (ESM Fig. 1).
SPs were induced by both 15 mmol/l glucose and 0.1 μmol/l glibenclamide, but not by 24 mmol/l KCl (Fig. 2a and ESM Fig. 2), suggesting that SPs depend on glucose-specific mechanisms such as closure of KATP channels. Glucose-induced SPs were suppressed by nifedipine, an inhibitor of voltage-gated calcium channels (Fig. 2b and ESM Fig. 2). We also tested the stress hormone adrenaline, an inhibitor of electrical signals in beta cells and an activator of alpha cells, at relatively high concentrations to ensure safe discrimination between the two cell types [10, 15–17]. SPs induced by glucose were reversibly inhibited by adrenaline (Fig. 2b). However, adrenaline did not affect the APs, presumably due to alpha cells being recorded together with beta cells on MEA electrodes (Fig. 2c). The dependence of SPs on elevated concentrations of glucose, which inhibit alpha cell function, indicates that SPs originated from beta cells (Fig. 2c).
We next monitored SPs in response to increasing and decreasing glucose ramps (Fig. 3a). The frequency of SPs recorded in the presence of various glucose concentrations was higher during the increasing (up) than the decreasing (down) ramp (Fig. 3b and ESM Fig. 3). SP frequency increased during the rising phase, with a median effective concentration (EC50) of 7.5 mmol/l and a maximal effect at 10 mmol/l glucose, and then it slowed down during the decreasing phase at a faster rate, resulting in a shift of the EC50 to 8.7 mmol/l. The difference in the kinetics during the increasing vs the decreasing glucose concentration resulted in a hysteresis of the up- and down-curve, which was also observed for the AP frequencies (not shown), consistent with a rapid response of beta cells to a decrease in glucose levels. We further observed that 0.5 pmol/l GLP-1 increased the frequency of SPs (Fig. 3c and ESM Fig. 3), with a maximal effect at 50 pmol/l, a concentration in the high physiological range [18].
We next asked whether SPs could be generated by individual beta cells or whether they required cell clusters. Glucose-induced SPs were not affected by 100 μmol/l of two inhibitors of purinergic receptors, namely suramin and pyridoxalphosphate-6-azophenyl-2′,4′-disulfonic acid (not shown). By contrast, 50 μmol/l carbenoxolone and 3.5 mmol/l heptanol-1 [19] abolished glucose-induced SPs in a reversible manner (Fig. 4a, b). Of note, glucose-induced APs were not inhibited by 50 μmol/l carbenoxolone, indicating that only SPs depend on gap junction channels.
To further test this hypothesis, we explored the electrical activity of islets from transgenic mice carrying a beta cell specific knockout of the gene encoding connexin 36 (Cx36), the main gap junction channel in beta cells [11]. Levels of CX36 protein and beta cell coupling for Cx36 flox(CFP) WT, Flox and RIP-Cre littermates were normal, half reduced and nil, respectively [11]. Raising glucose from 3 mmol/l to 15 mmol/l induced SPs and APs in islets of WT, but not in those of Flox and RIP-Cre mice (Fig. 4c). Thus, a 50% decrease in CX36 protein levels is sufficient to compromise the generation of SPs.
In view of the differences in ion channels in rodent and human beta cells [1], we searched for SPs in islets isolated from three organ donors (ESM Table 1). We found that glucose generated APs and SPs in human islets (Fig. 5a), the maximal effect being reached at 8.2 mmol/l (Fig. 5b). As in mouse islets, human SPs were reversibly suppressed by heptanol-1 (not shown). Continuous long-term recordings showed that, in human islets, glucose induced SPs with a very stable frequency over a 24 h period (Fig. 5c).
Discussion
Our study has revealed slow extracellular field potentials induced by glucose in mouse and human islets. As depicted in Fig. 6, SP signals add to the previously described APs [9, 10, 20] when beta cells are coupled by CX36 channels [21, 22]. APs are generated at the single-cell level [1], whereas SPs result, like local field potentials in neurons, from complex temporal and spatial summations of ion fluxes around each electrode [23]. The asymmetric temporal partition of APs on SPs (Fig. 1g) suggests a correspondence between the falling phase of SP and a depolarising stage of coupled beta cells. In fact, SPs and APs are observed at elevated glucose levels provided that beta cells contact the electrode and are coupled via connexins (Fig. 6a). By contrast, only APs are recorded at elevated glucose levels when coupling is disrupted by pharmacological or genetic intervention (Fig. 6b). At low glucose levels, when alpha cells are activated, we observed only APs in some electrodes, but never SPs (Fig. 6c; see also Fig. 2c). SPs generated by the surrounding beta cells become apparent at elevated glucose levels. Thus, SPs reflect the electrical activity and coupling of beta cells and provide important insights on the endogenous algorithms used by islets to encode the insulin demand.
The presence of APs in activated beta cells is well known [1]. They can be recorded using arrays of extracellular electrodes [9, 10, 20, 24] and their direction depends on factors such as strength of cell adhesion and the local population of ion channels at the cell-electrode interface [25]. The MEA approach reduces the signal/noise ratio of APs. Consequently, registered amplitudes are significantly smaller than those reported by classical intracellular electrophysiological methods [9, 10, 20, 22, 24, 26]. This altered signal/noise ratio renders signal recognition more difficult and requires sophisticated approaches such as wavelets and dynamic filtering [10, 27]. By contrast, the large amplitude of SPs provides easier recognition with increased precision. Similar signals have previously been recorded in rodents using single electrodes, which precludes large and statistically reliable sampling [28]. This limitation is solved using MEAs [7–10, 12, 20, 24]. Our data show that this approach is also applicable to human islets despite the many electrophysiological differences [29, 30], and that SPs remain stable during 24 h continuous recording.
Several lines of evidence indicate that SPs reflect the functional coupling of beta cells. Since antagonists of purinergic receptors did not alter SPs, the paracrine effect of the ATP co-released with insulin [31, 32] is not involved. SPs but not APs were suppressed by pharmacological inhibitors of connexins or knockout of Cx36, encoding the only gap junction protein shown so far to couple beta cells [11, 33]. Indeed, a 50% decrease in the control islet protein levels of CX36 prevents the generation of glucose-induced SPs as shown here and largely reduces glucose-induced insulin secretion [11], suggesting a close link. Although CX36 is dispensable for the occurrence of oscillations in cytosolic calcium, it is required to synchronise the period and amplitude of these oscillations between cells of an islet [33]. Thus, SPs recorded on MEAs reflect the propagation of extracellular current flow between coupled cells, which makes them an excellent marker for normal islet function.
The physiological relevance of SPs is supported by their induction via the activation of stimulus-secretion coupling typical for beta cells [1, 29]. SPs were induced by glucose and sulfonylurea secretagogues causing both the closure of KATP channels and Ca2+ oscillations [34–36]. By contrast, SPs are not evoked by high concentrations of KCl, which probably prevents propagation by clamping all cells at the same potential. Finally, the L-type channel blocker nifedipine abolished SPs, demonstrating that calcium currents underlie these potentials. As calcium influx through L-type channels is also the main trigger of exocytosis, SPs are operationally linked to the secretory response of beta cells.
SPs were maximally stimulated in the presence of 10 mmol/l glucose. This concentration is lower than that which usually elicits in vitro, in a single or few steps, maximal insulin secretion [32, 37], but it is similar to that which attains the same secretory effect using a glucose ramp [38] or had been reported from in vivo patch-clamp experiments in anaesthetised mice [39]. The glucose concentrations inducing the maximal frequency of SPs coincide with those observed in humans during an OGTT [40]. GLP-1 and adrenaline increased and decreased SP frequencies, respectively, consistent with their effects on insulin secretion. Note that GLP-1 was effective at physiological picomolar concentrations as found during the postprandial period [18].
Many glucose-related events feature slow and rapid oscillations in islet cells [4, 41]. Concomitant oscillations of membrane potential and of Ca2+ spreading over the islet have been reported recently in mouse pancreatic tissue slices [42]. Although the interval between two oscillations appears longer than for SPs, the duration of these oscillations and of SPs are quite similar [42]. Our data show that the frequency of SPs immediately depends on the ambient concentration of glucose and GLP-1. Thus, extended recording periods, such as those used in the determination of burst durations, are not necessary to evaluate beta cell function, which consequently can be determined on-line in real-time. In addition, bursts are less marked in human islets as compared with rodent islets [29]. We, therefore, propose that the overall frequency of SPs generated by the interplay of multiple cells within each islet is sufficient to encode the amount of insulin to be secreted. This also provides a novel algorithm that largely reduces computing time for a bio-artificial sensor that might mimic islet cells in real-time.
A further interesting observation concerns the difference of the effect of glucose on SPs during an increasing and a decreasing ramp. The curve is hysteretic, reflecting a reduced sensitivity of the signals towards decreasing glucose levels, reminiscent of that described in man during GTTs [40]. This control is presumably beneficial to prevent hypoglycaemia as a consequence of a sustained insulin release. As hysteresis was present in isolated islets, this safety mechanism is encoded already in the micro-organ and may occur independently from other regulatory circuits.
In conclusion, our work has identified SPs as a novel biomarker for the rapid and non-invasive testing of isolated islets and islet cell clusters. These novel ‘coupling waves’ specifically reflect the function of beta cells and provide for a robust signal whose detection does not require complex transformations and filtering [43, 44]. Since SPs depend on CX36 expression, which influences the differentiation and secretory function of rodent and human islets [45–47], they should provide a suitable marker for beta cell monitoring, e.g. prior to transplantation of intact islets or clusters of surrogate insulin-producing cells. The recording of signals after 6 h is fully compatible with current transplantation schemes after overnight culture [48]. In addition, differentiation of stem cells into islet cells requires complex protocols and on-line monitoring as presented here may be of considerable advantage. The approach may also help to improve algorithms and sensors needed for the development of a therapeutically valuable artificial pancreas [49].
Abbreviations
- AP:
-
Action potential
- CX36:
-
Connexin 36
- EC50 :
-
Median effective concentration
- Flox:
-
Cx36 Flox(CFP)/Flox(CFP)
- GLP-1:
-
Glucagon-like peptide-1
- GTT:
-
Glucose tolerance test
- KATP :
-
ATP-dependent potassium
- MEA:
-
Multielectrode array
- OGTT:
-
Oral glucose tolerance test
- RIP-Cre:
-
Cx36 Flox(CFP)/Flox(CFP):RIP-Cre
- SP:
-
Slow potential
- WT:
-
Wild-type
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Acknowledgements
Human islets were provided through the JDRF award 31-2008-416 (ECIT Islet for Basic Research program). We thank E. Pedraza (University of Bordeaux, Bordeaux, France) for critical reading of the manuscript. We gratefully acknowledge the help of the Animal Facility of the University of Bordeaux.
Funding
This work was supported by grants from the French Ministry of Research (JL), FEDER BIODIA (JL and BC), the Aquitaine Regional Council (JL and BC), the French Research Agency (ANR-10-EMMA-030 and ANR-13-PRTS-0017-06; JL and SR), and the Swiss National Science Foundation (310030_141162; PM). Within the framework of LabEx AMADEus (ANR-10-LABX-0042-AMADEUS), this work has benefitted from aid by the ANR under programme Initiative for Excellence IdEx Bordeaux (ANR-10-IDEX-0003-02).
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FL and MR designed research, performed experiments, researched data, and contributed to discussion and writing of the manuscript. JL designed research and contributed to discussion and writing of the manuscript. YB, IB and AP performed experiments, analysed data and contributed to writing of the manuscript. DB, TB, PM, BC and SR contributed to the design of the work and discussion. JL is the guarantor of this work, has had full access to all the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis. All authors contributed to the revision and approved the final version.
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M. Raoux and J. Lang contributed equally to this work.
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Lebreton, F., Pirog, A., Belouah, I. et al. Slow potentials encode intercellular coupling and insulin demand in pancreatic beta cells. Diabetologia 58, 1291–1299 (2015). https://doi.org/10.1007/s00125-015-3558-z
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DOI: https://doi.org/10.1007/s00125-015-3558-z