Abstract
The Hsp70 and Hsp90 molecular chaperone systems are critical regulators of protein homeostasis (proteostasis) in eukaryotes under normal and stressed conditions. The Hsp70 and Hsp90 systems physically and functionally interact to ensure cellular proteostasis. Co-chaperones interact with Hsp70 and Hsp90 to regulate and to promote their molecular chaperone functions. Mammalian Hop, also called Stip1, and its budding yeast ortholog Sti1 are eukaryote-specific co-chaperones, which have been thought to be essential for substrate (“client”) transfer from Hsp70 to Hsp90. Substrate transfer is facilitated by the ability of Hop to interact simultaneously with Hsp70 and Hsp90 as part of a ternary complex. Intriguingly, in prokaryotes, which lack a Hop ortholog, the Hsp70 and Hsp90 orthologs interact directly. Recent evidence shows that eukaryotic Hsp70 and Hsp90 can also form a prokaryote-like binary chaperone complex in the absence of Hop, and that this binary complex displays enhanced protein folding and anti-aggregation activities. The canonical Hsp70-Hop-Hsp90 ternary chaperone complex is essential for optimal maturation and stability of a small subset of clients, including the glucocorticoid receptor, the tyrosine kinase v-Src, and the 26S/30S proteasome. Whereas many cancers have increased levels of Hop, the levels of Hop decrease in the aging human brain. Since Hop is not essential in all eukaryotic cells and organisms, tuning Hop levels or activity might be beneficial for the treatment of cancer and neurodegeneration.
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Introduction
The 90 kDa and 70 kDa heat shock proteins (Hsp) Hsp90 and Hsp70, respectively, are ATP-dependent molecular chaperones essential for eukaryotic life. Hsp70 is composed of two main domains: the N-terminal nucleotide-binding domain (NBD), and the C-terminal substrate-binding domain (SBD), which associates with substrate proteins by recognizing short exposed hydrophobic stretches in the initial stages of substrate folding [1,2,3,4]. Hsp70-driven protein folding is a concerted process with Hsp40 and a nucleotide exchange factor (NEF) [1, 2, 4]. Moreover, Hsp70 prevents protein aggregation, solubilizes aggregated proteins, and assists in clearing misfolded proteins and aggregates primarily via chaperone-mediated autophagy (CMA) [1, 2, 5,6,7].
The Hsp90 chaperone system works downstream of the Hsp70 chaperone system and is responsible for the final stages of folding and maturation of substrate proteins (discussed later in detail). There are two cytosolic isoforms of Hsp90 identified in mammals, Hsp90α and Hsp90β, and in budding yeast, Hsp82 and Hsc82, the inducible and constitutively expressed isoforms of Hsp90, respectively [8,9,10]. Hsp90 paralogs are found in mitochondria (Trap1) and the endoplasmic reticulum (Grp94 or Gp96) along with organelle-specific paralogs of Hsp70 (Mortalin in mitochondria and Grp78 in the endoplasmic reticulum) [5, 10]. Hsp90 isoforms exist as dynamic homodimers. Each protomer of the homodimer is composed of three highly conserved domains: the N-terminal domain (NTD) involved in nucleotide (ATP) binding and hydrolysis, the middle domain (MD), which contributes the catalytic lysine residue for the ATPase activity and is responsible for binding many of the substrates, and the C-terminal domain (CTD) responsible for dimerization [9,10,11,12]. The substrate proteins are collectively called Hsp90 “clients” [4, 9, 11,12,13,14]. The current set of Hsp90 clients includes kinases, transcription factors, including steroid receptors, and many other proteins, sharing no common features in terms of sequence or structure [9, 10, 12]. A comprehensive and continuously updated list of Hsp90 clients and other interactors is available at https://www.picard.ch/downloads/Hsp90interactors.pdf [15].
Hsp90-mediated client protein folding or assembly is a highly regulated process, which requires the sequential association and release of co-chaperones [9, 10, 12]. One of these co-chaperones is Hsp70–Hsp90 organizing protein (Hop), encoded by the gene STIP1 in vertebrates and STI1 in yeast. Justifying its name, Hop is an adaptor protein between Hsp70 and Hsp90 [10, 16]. It is proposed that Hop facilitates the client transfer from the Hsp70 to the Hsp90 chaperone systems and thereby promotes the protein folding process.
This review will discuss the significant discoveries around Hop in the last three decades and the progress in the understanding of its molecular functions and physiological relevance. We mainly focus on the role and regulation of Hop and its impact on the Hsp70–Hsp90 molecular chaperone machines and protein homeostasis (proteostasis). Moreover, we propose the possible implications of modulating Hop as a therapeutic target in cancer and neurodegenerative diseases. A continuously updated literature overview on Hop is available at https://www.picard.ch/downloads/Hopfacts.pdf.
Discovery, expression, and localization of Hop
The gene for Hop was first discovered in budding yeast as a stress-inducible gene, hence named STI1 (or Sti1 for the protein) in that species [17]. Consistent with its heat shock-induced overexpression, the core heat-shock element (HSE) TTC-GAA-TTC-GTA was found at position − 241 of the promoter region of the STI1 gene [17, 18]. The yeast STI1 gene was found to encode a polypeptide of 589 amino acid residues with a calculated molecular mass of 66.246 kDa [17]. However, the experimental molecular mass of Sti1 was determined to be approximately 73 to 75 kDa by SDS-PAGE analysis [17]. Two-dimensional gel electrophoresis revealed four isoforms with isoelectric points from 5.75 to 6.05 in yeast [17]. This finding indicated that the endogenous Sti1/Hop protein might be post-translationally modified, for example by differential phosphorylation. In this context, it is noteworthy that Hop is also known as stress-induced phosphoprotein 1 (Stip1) in mammals. Hop was first identified and characterized in a human cell line as an SV40 transformation-induced protein [19]. Human Hop is a 543-amino acid protein with a calculated molecular mass of 62.6 kDa [19]. Sequence alignments revealed 42% amino acid sequence identity between the orthologs of Hop from humans and yeast [19].
Although Sti1/Hop was discovered as a stress-induced gene, the mechanism of this regulation is not well understood. The existence of an HSE in the promoter sequence of STI1 would allow the binding of Hsf1, the master transcriptional regulator of the heat-shock response (HSR). Inhibition of Hsp90 in human gastric cancer cells can also induce the HSR by activating Hsf1, which is then recruited to the promoters of STIP1 and other target genes, including BAG3, HSPA1A, HSPA1B, FKBP4, and UBB [20]. Alternatively, under hypoxic stress in primary cortical cultures from mice, transcriptional activation of STIP1 was shown to be driven by HIF1α, the master regulator of the hypoxic response [21]. A hypoxia response element (HRE) was found at positions -60 to − 63 of the promoter and thought to be responsible for its expression during oxygen deprivation in mammalian cells [21]. Furthermore, the HRAS oncogene and mutant p53 positively regulate the transcription of STIP1, whereas wild-type p53 inhibits it [22]. Although the expression of Hop under stress conditions has been explained, how it is regulated under normal physiological conditions remains to be investigated. Moreover, the translational control of Hop and the functions of the 5′- and 3′-UTRs of its mRNA need to be studied under both normal physiological and stress conditions.
Hop is primarily localized in the cytosol [23]. However, heat-shock treatment and hydroxyurea-induced G1/S cell cycle arrest promote nuclear localization of Hop [24, 25]. These findings suggest that Hop shuttles between the cytosol and the nucleus. The post-translational modifications regulate the subcellular localization of Hop. There is evidence that there are two distinct phosphorylation sites for murine Hop, S189 and T198, which are phosphorylated by casein kinase II and Cdk1, respectively [26]. Intriguingly, whereas S189 phosphorylation promotes nuclear localization of murine Hop, T198 phosphorylation restrains it in the cytosol [24]. Since a cell cycle-specific kinase (Cdk1) phosphorylates Hop, it is proposed that Hop can differentially localize in cells depending on the cell cycle phases. This further raises the possibility of cell cycle phase-specific functions of Hop, an idea that is supported by the observation that a Hop knockdown or knockout in human cancer cells leads to a slowdown in G1 [27,28,29]. Interestingly, the phosphorylation sites of Hop are conserved between its murine and human orthologs. Although these phosphorylation sites are well-characterized for murine Hop, functions of these sites are yet to be described for the human ortholog, which only differs by 14 amino acids. Mammalian Hop can also be SUMOylated by PIAS1, an E3 SUMO ligase [30]. Interestingly, the interaction with PAIS1 promotes the nuclear localization and retention of Hop independently of SUMOylation [30].
Apart from the cytosol and the nucleus, a fraction of the intracellular Hop resides in the Golgi apparatus [19] and at the cell membrane [31, 32]. Many cell types can also secrete Hop. Glial cells and astrocytes secret Hop as a neurotrophic ligand, which triggers prion protein (PrPc) signaling in neurons with neuroprotective effects [32,33,34,35]. Hop was also reported to be secreted by hepatocellular, ovarian, brain, and gastric carcinoma cells and tissues [36,37,38,39,40]. For ovarian and gastric carcinoma, secreted Hop was proposed to be a diagnostic and prognostic marker [38, 39, 41]. Additionally, Hop is present in extracellular vesicles or exosomes released by mouse embryonic stem cells and astrocytes, and is thought to play a role in intercellular communication [42, 43]. Hop, together with Hsp90, was even shown to play an active role in promoting the release of exosomes in a Drosophila model system [44]. The secreted Hop and its cytokine-like functions in CNS development and cancer have recently been reviewed [45]. Here, we will focus on the intracellular and physiological functions of Hop.
Structure of Hop
A prediction of the Sti1 protein structure was first proposed back in 1989 along with its discovery [17]. Its predicted amino acid sequence showed it to be a rather hydrophilic protein [17]. Secondary protein structure prediction indicated that most of the regions of Sti1 exist in α-helical domains [17]. Sti1/Hop contains three tetratricopeptide repeat (TPR) domains and two aspartate-proline-rich polypeptide (DP) segments [16, 19, 46] (Fig. 1a). TPR domains comprise three or more TPR motifs (tandem array of two antiparallel α‐helices), and each motif consists of 34 amino acid repeats [47]. The TPR domains of Sti1/Hop are called TPR1, TPR2A, and TPR2B, while the two DP domains are named DP1 and DP2 [48]. Overall, structurally, the TPR domains are highly organized α-helices [47]. The TPR1, TPR2A, and TPR2B domain structures of yeast and human Sti1/Hop were evaluated either by X-ray diffraction or solution NMR [16, 49,50,51]. These studies experimentally confirmed the predicted α-helical structures of the TPR domains of Hop. The DP domains were considered to be unstructured until solution NMR spectroscopy revealed highly homologous α‐helical folds with six helices in DP1 and five helices in DP2 of Sti1 [49]. While the individual domain structures of yeast and human Sti1/Hop are known, the structure of full-length Sti1/Hop has yet to be solved. Several studies reported Hop to be a dimeric protein [52,53,54,55]; however, this was contradicted by others claiming that Hop is a monomeric protein [56, 57]. A cryo-EM analysis of a Hop-Hsp90 complex suggested that a Hsp90 dimer binds only one Hop molecule [58].
Hsp70 and Hsp90: the major interactors of Hop
Hop was first discovered as one of the major interactors of Hsp90 by immunoaffinity purification of Hsp90 from chicken oviduct cytosol [46]. This Hsp90-Hop complex was found to be simultaneously associated with Hsp70. This finding strongly suggested the existence of a Hsp70-Hop-Hsp90 ternary complex in the eukaryotic cytosol (Fig. 1a). Although immunoaffinity purification of Hsp90 co-precipitates substoichiometric amounts of Hsp70 and Hop [46], we recently found that the use of an antibody to (HA-tagged) Hop co-immunoprecipitates almost equimolar amounts of Hsp70 and Hsp90 from HEK293T cells [28] (Fig. 1b). This indicated that most of the intracellular Hop molecules are complexed with Hsp70 and Hsp90 [28]. However, evidence has been emerging that intracellular Hop can interact with other proteins independently of its binding to either Hsp70 or Hsp90 or both [59,60,61,62,63,64].
What is the molecular basis for the simultaneous interaction of Hop with Hsp70 and Hsp90? The TPR1 domain of Hop is responsible for the binding to the extreme C-terminal heptapeptide PTIEEVD of Hsp70 (Fig. 1a). TPR2A serves as a high-affinity binding site for the extreme C-terminal peptide MEEVD of Hsp90 [16] (Fig. 1a). The conserved EEVD motifs at the C-termini of Hsp70 and Hsp90 behave like a dicarboxylic acid clamp and are responsible for the electrostatic interactions with the respective TPR domains of Hop [16, 49, 65,66,67]. The selectivity and specificity of binding of the respective TPR domains to the C-termini of Hsp70 and Hsp90 are conserved between humans and yeast [16, 49].
Unlike TPR1 and TPR2A, the TPR2B domain of Sti1/Hop can bind both Hsp70 and Hsp90 without a marked preference for either one of the C-termini, but its binding is of relatively low affinity [49]. Thus, Sti1/Hop has one high-affinity binding site each for Hsp90 (TPR2A) and Hsp70 (TPR1), and an additional, less selective Hsp70/Hsp90 binding site (TPR2B). Hsp70 may initially bind to the TPR1 domain of Sti1/Hop in an open conformation, and then transfer to TPR2B once Hop binds Hsp90 via TPR2A [68]. Moreover, additional contact sites with the CTD and MD of Hsp90 are also evident [49, 55, 69,70,71].
Different biophysical techniques, including surface plasmon resonance, isothermal calorimetry, analytical ultra-centrifugation, hydrogen exchange mass spectrometry, and luminescence resonance energy transfer, have been used to determine and characterize the affinities [dissociation constants (KD)] of the respective TPRs of Hop for the C-terminal sequences of Hsp70 and Hsp90 [16, 48, 49, 72, 73]. Tomm34, another multiple TPR domain-containing protein and Hsp90 co-chaperone of the outer mitochondrial membrane, has also been shown to bind simultaneously to Hsp70 and Hsp90 [74]. However, Hop cannot be functionally substituted by Tomm34, most notably in Hop knockout cells because the abundance of Tomm34 is too low [28]. Although it appears that Hop cannot be functionally replaced in the Hsp70–Hsp90 chaperone complex by another TPR-containing protein, Hop competes for Hsp90 binding with several other TPR domain-containing proteins, such as cyclophilins, including FK506 binding proteins (FKBPs) or peptidyl-prolyl isomerase (PPIase), and Stub1/Chip [75,76,77]. In fact, there is evidence that mixed complexes such as Hsp90-FKBP52-Hop-Hsp70, Hsp90-FKBP52-p23-Hop, and Hop-PPIase-Hsp90 also exist in cells, albeit in reduced proportions [54, 57, 77, 78]. It was proposed that serine/threonine phosphorylation just N-terminal to the EEVD motifs of the C-termini of Hsp70 and Hsp90 enhances the binding to Hop and prevents the binding to Stub1/Chip [79]. Posttranslational modifications on either Hsp70 or Hsp90 or the co-chaperones influence interaction preferences and need to be studied more thoroughly in the future.
Impact of Hop on the Hsp70–Hsp90 chaperone cycle and client maturation
Hsp70 and Hsp90 are the evolutionarily conserved molecular chaperones that assist in the folding of nascent polypeptides and stress-induced misfolded proteins in the cytosol [4, 10]. Substrates are recognized by Hsp70 and a J-domain containing Hsp70 co-chaperone, typically Hsp40 in eukaryotes [5, 80, 81] (Fig. 2a, b). J-domain protein (JDPs) such as Hsp40 stimulate the Hsp70 ATPase activity and enhance the substrate binding affinity of Hsp70 by promoting its conversion to the ADP-bound state [82]. The NEF-dependent ADP release from Hsp70 allows the binding of ATP, which triggers its substrate release [1]. Although Hsp70 was thought to be a solitary molecular chaperone for protein folding and refolding, the hydrophobic properties of its substrate-binding cavity could even arrest folding for some substrates [4]. A recent finding experimentally supports the idea of Hsp70 acting as a preventer of protein folding. It was discovered that Hsp70, when tested at physiological concentrations in vitro, inhibits the folding of a model substrate [83]. Because of its hydrophilic nature, Hsp90 does not block protein folding as Hsp70 does [4, 83]. Thus, Hsp90 becomes essential to break this “deadlock” of the Hsp70 chaperone system by allowing the completion of the protein folding process and the subsequent release of active substrates [83]. As a result, Hsp70 and Hsp90 functionally collaborate to enhance the yield of folded proteins [83] (Fig. 2a, b).
In eukaryotic cells, the cooperation between Hsp70 and Hsp90 is mediated by Hop, as discussed above. Formation of the Hsp70-Hop-Hsp90 ternary chaperone complex may be the rate-limiting step for the substrate transfer from the Hsp70 system to the Hsp90 system [68] (Fig. 2a). Upon substrate binding, Hsp90 undergoes large conformational changes concerted with the sequential and highly regulated binding of other co-chaperones, including Aha1, PPlase, and p23 [10, 12, 84, 85] (Fig. 2a). Aha1 binds to the MD of Hsp90 and stimulates its ATPase activity, which is a prerequisite for substrate protein folding and subsequent release [86]. Two molecules of p23 bind to the NTDs of the Hsp90 dimer in its closed state and facilitate the final steps of substrate-protein folding, maturation, and release in a concerted manner with Aha1 [87,88,89,90] (Fig. 2a). After completion of a successful ATPase cycle, the Hsp90 dimer transforms into an open conformation and is restored for the next chaperone cycle (Fig. 2a). Each co-chaperone acts uniquely on the ATPase activity of Hsp90 and the conformational dynamics of the entire Hsp90 dimer [10, 12, 84, 85, 91].
Hop is the physiological allosteric inhibitor of the ATPase activity of Hsp90 [53, 57, 65]. Hop does so probably by stabilizing the open conformation of the Hsp90 dimer and blocking the N-terminal closure of Hsp90, which is required for its ATPase activity [65]. Although Hop blocks the ATPase activity of Hsp90, it does not inhibit ATP binding to the NBD of Hsp90 [65]. TPR2A-TPR2B is the minimally required segment of Hop for maximum inhibition of the ATPase activity of Hsp90 [48, 49]. TPR2B of Hop/Sti1 occupies a position at the MD of Hsp90, between the two protomers of the Hsp90 dimer, thereby sterically restricting the conformational rearrangement of the MD and NTD closure during the ATPase cycle [49]. A cryo-EM analysis showed that the TPR1 domain of Hop could also sterically block the Hsp90 N-terminal dimerization by being situated between the Hsp90 protomers and interacting with the adjacent NTD and MD [58].
By itself, the TPR2A–TPR2B domains of Hop are unable to facilitate the activation of Hsp90 clients in vivo despite the fact that this portion of Hop retains the full inhibitory capacity for the ATPase activity of Hsp90 [49]. For functionality it needs DP2. The TPR2A–TPR2B–DP2 portion of Hop is the minimally required functional module to recapitulate most of the in vivo activities of full-length Hop for Hsp90 client protein folding and maturation [49]. Remarkably, the TPR1 domain of Hop was proposed to be dispensable for Hsp90-client protein folding [49]. This is further supported by the fact that the Caenorhabditis elegans Hop ortholog lacks a TPR1 domain and yet is fully functional [92,93,94]. In contrast to the inhibitory effect of Hop on the ATPase activity of Hsp90, Sti1 binding enhances the ATPase activity of Hsp70, thereby promoting the activity of Hsp70 in yeast [95]. Human Hop is unable to activate the ATPase of human Hsp70 since its basal ATPase activity is higher to begin with [95]. A genetic analysis of the determinants of Sti1-dependence of Hsp90 in budding yeast yielded a wealth of Hsp90 point mutants [96]. Overall, the results are also consistent with Sti1 promoting the recruitment of client-loaded Hsp70 and client transfer through a conformational change of Hsp90 favoring its N-terminal closure [96].
Little is known about the relevance of posttranslational modifications of Hop for Hsp70–Hsp90 functions. A study regarding a few phosphorylation sites of human and yeast Hop revealed that, in general, phosphorylation of Hop is counterproductive for the Hsp70–Hsp90-driven client protein folding and maturation processes [97, 98] (https://www.phosphosite.org/proteinAction.action?id=2638&showAllSites=true). Further studies are warranted to clarify additional functional modifications of Hop, the upstream regulators, and their relevance for cellular physiology and disease pathology.
One interesting question is whether Hop by itself can act as a molecular chaperone. Efficient conversion of chemical energy into mechanical energy was thought to be the prerequisite for a protein to be a molecular chaperone. Recently, this idea has been experimentally proven for the Hsp70 chaperone system [99]. Although Hop was reported to bind ATP and to have a slow ATPase activity [100], Hop is unable to fold substrates by itself [52, 101]. Therefore, since Hop fails to fulfill the criteria for being a molecular chaperone, it must be considered a co-chaperone of the Hsp70–Hsp90 chaperone system.
Hsp70-Hop-Hsp90 ternary complex versus Hsp70–Hsp90 binary complex
Hop appeared during the evolution of eukaryotic organisms [17, 102] whereas no Hop-like protein has been discovered in bacteria. Bacterial Hsp70 (DnaK) and Hsp90 (HtpG) must, therefore, be able to interact directly during substrate transfer [103,104,105,106,107]. The Escherichia coli (E. coli) Hsp70–Hsp90 binary complex is further stabilized by the interaction with both clients and a DnaJ protein, the bacterial JDP [104]. Mutating surface residues that are essential for the direct interaction between E. coli Hsp70 and Hsp90 impair binary complex formation and prevent substrate protein folding [104]. A recent computational model places the client proteins bound to the SBD of bacterial Hsp70 in the proximity of the client-binding site of bacterial Hsp90, which may facilitate substrate transfer from Hsp70 to Hsp90 [105]. The direct interaction of these bacterial molecular chaperones mutually and synergistically enhances their ATPase activity in the presence of substrate proteins [104,105,106].
The appearance of Hop in eukaryotes correlates with the modification of the C-termini of eukaryotic cytosolic Hsp70 and Hsp90, such that they contain the extreme C-terminal EEVD motif allowing Hop binding (Fig. 1a). And yet, recent discoveries show that eukaryotic Hsp70 and Hsp90 can interact directly in the absence of Hop both in vitro and in vivo [28, 108] (Fig. 2b). This Hop-independent Hsp70–Hsp90 binary complex is functionally active for substrate folding/refolding and even with a higher rate and yield [28] (Fig. 2b). Since Hop is a physiological inhibitor of the ATPase activity of Hsp90, we proposed that the Hsp70–Hsp90 binary complex has a higher folding capacity than the Hsp70-Hop-Hsp90 ternary complex. This may be the case, at least for some substrates, because the binary complex is not restrained anymore by Hop (Fig. 2b) [28].
Conserved surface residues that are essential for the direct interaction of bacterial Hsp70 and Hsp90 are also essential for the eukaryotic Hsp70–Hsp90 binary complex formation and for substrate refolding [28]. Therefore, Hsp70 and Hsp90 retain their ability to form a more ancient prokaryote-like functional chaperone complex even in eukaryotes (Fig. 2b). Further details of the functional aspects of these binary and ternary molecular chaperone complexes are discussed in the following sections.
The endoplasmic reticulum and mitochondria, which have paralogs of Hsp70 and Hsp90, lack Hop or Hop-like proteins. Their Hsp70 and Hsp90 physically and functionally interact directly [109, 110, see also ref. 111] and also lack the extreme C-terminal EEVD motif. Whether Hsp70 and Hsp90 can interact directly in the eukaryotic cytosol in the presence of Hop and whether this alternative binary chaperone complex has any specialized functions are still open questions. Caenorhabditis elegans could be an interesting model organism to test this notion since its Hop lacks the TPR1 domain and competitively interacts with Hsp70 and Hsp90 [92, 93]. For Caenorhabditis elegans, it remains to be determined whether Hsp70 and Hsp90 interact directly or whether the TPR2A-TPR2B domains of Hop are sufficient to form a functional Hsp70-Hop-Hsp90 ternary complex.
Phenotypes of Hop mutants in eukaryotic organisms
Since Hop is specifically present only in eukaryotic organisms, and strictly required for the client transfer to Hsp90 and folding in some biochemical assays, Hop was thought to be indispensable for eukaryotic life. Therefore, deletion or dominant-negative mutations of the Hop encoding genes could be expected to be lethal. Contradicting this speculation, we now know that budding yeast, worms (Caenorhabditis elegans), flies (Drosophila melanogaster), and human cancer cell lines are viable without Hop [17, 28, 93, 112]. Whereas ∆sti1 yeast strains are viable, but heat- and cold-shock sensitive [17]. These mutant yeast strains as well as human cancer cells lacking Hop are somewhat more dependent on Hsp90 functions since they are hypersensitive to Hsp90 inhibitors [28, 113,114,115,116]. ∆sti1 strains are also hypersensitive to molybdate, which stabilizes Hsp90 in the closed conformation even after ATP hydrolysis and is thought to constrain the dynamic rearrangements of Hsp90 [117]. This agrees with the fact that deletion of either hsc82 or hsp82 is synthetically lethal with ∆sti1 in yeast under normal growth conditions or at elevated temperature, respectively [76]. In line with these notions, a series of temperature-sensitive mutations in the MD of yeast Hsp90, which are defective for the interaction with yeast Hsp70, have been shown to be synthetically lethal with ∆sti1[108]. Several other mutations in yeast have been discovered to be synthetically lethal with ∆sti1 including cpr7, cdc37, ydj1, mps1, sse1, sba1, ssl2, emc2, and utp21 [63, 116, 118,119,120,121,122,123].
Caenorhabditis elegans is viable in the absence of Hop, but the null mutant has decreased fertility, a shortened lifespan, and an impaired heat-shock response [93]. Unlike yeast and worm models, several Hop knockout human cancer cell lines are either equally or rather less sensitive to acute heat shock compared to their wild-type counterparts [28]. One can imagine that the relatively hyperactive Hsp70–Hsp90 binary complex in the absence of Hop might be protective upon heat shock. In contrast, the knockdown of Hop in human HEK293T cells with a doxycycline-inducible system was reported to reduce viability under basal and heat-shock conditions [124]. These differences between the knockout and knockdown models are striking and may be explained by the long-term adaptation to the Hop depletion during the clonal establishment of Hop knockout cells. This apparent discrepancy will have to be resolved methodically in the future. The sensitivity to other types of stresses [125,126,127] may also depend on the exact experimental conditions and on cell type, and will require further investigations.
Unlike the above-mentioned models of Hop mutants, Hop appears to be essential for mouse embryonic development [125] and for the viability of Leishmania donovani promastigotes [128]. Hop knockout mouse embryos die at the stage of E10.5; the knockout causes improper neural tube formation, increased DNA damage and stress susceptibility, and apoptosis, which may all contribute to embryonic lethality [125]. Even heterozygotes display increased cellular stress and are more sensitive to cerebral ischemia [125]. Mice with a Hop TPR1 truncation can be born, but they have a high mortality rate, possibly because the truncation mutant accumulates to considerably lower levels [126]. These mouse models clearly demonstrate that not every eukaryotic cell may be able to shift from the Hsp70-Hop-Hsp90 ternary complex to the Hsp70–Hsp90 binary complex functionally, and that even a reduced level of functional Hop is detrimental to the adult life in mouse. It has been proposed that the absence of extracellular and not just intracellular Hop in these mouse models might contribute to the lethality of neuronal cells [125]. However, the details of the molecular targets and mechanisms behind the death of the mouse embryos lacking Hop warrant further investigations.
Hop as a regulator of proteostasis
Proteostasis is the overall process by which the cellular proteome is maintained in a functional state. All axes of proteostasis, including protein translation, folding/refolding, and degradation, are extensively regulated by molecular chaperones [129,130,131,132]. Both Hsp70 and Hsp90 are collectively involved in ribosomal biogenesis, translation, and co-translational protein folding [133,134,135,136,137,138]. Multidomain cytosolic proteins typically undergo chaperone-assisted protein folding by Hsp70 and Hsp90. Hsp90 can also transiently hold structurally labile or misfolded proteins to prevent their aggregation [139], similarly to how small Hsps use their “holdase” function [140]. Intriguingly, Hsp70 and Hsp90 are not only responsible for protein synthesis and stability, but they also regulate protein degradation. Hsp70 is the master regulator of chaperone-mediated autophagy (CMA) of degradation-prone substrate proteins carrying a KFERQ motif as a recognition peptide signal sequence [130]. With the assistance of its co-chaperone proteins, Hsp70 delivers the substrate proteins to lysosomes via an interaction with a lysosomal receptor LAMP2A [6, 7]. Although Hsp90 and its co-chaperones were also proposed to be involved in the CMA process, the molecular basis has yet to be revealed [7, 141].
Hsp90 is involved in protein degradation by the proteasome [28, 142]. Hsp90 was proposed to be a regulator of proteasome assembly and stability, and to facilitate proteasome reassembly after a heat shock-induced disassembly in yeast [142]. In yeast, mutations in several proteasomal genes were found to be synthetically lethal with hsp82 and hsc82 mutations [142]. Intriguingly, co-expression of Hsp90 is essential for the efficient formation of the recombinant proteasomal 19S lid complex in bacteria [143]. Several proteasome-dedicated chaperones are known for the 20S catalytic core and 19S base assembly, only Hsp90 has been proposed as a chaperone for 19S lid assembly to date [143]. Beyond the putative function of Hsp90 in lid assembly, for specific substrate proteins, Hsp90 serves as a chaperone for direct loading into the free 20S proteasomal core complex for degradation [144]. Thus, the Hsp70 and Hsp90 molecular chaperone systems are not only protein folding hubs, but they are also involved in many other aspects of proteostasis.
Hop in protein degradation We recently found that Hop promotes proteasome assembly and protein degradation [28] (Fig. 3), and that this novel function of Hop is evolutionarily conserved between humans and yeast [28]. The ability of Hop to form the Hsp70-Hop-Hsp90 ternary chaperone complex is essential for this function [28] (Fig. 3). Hop TPR domain mutants, that can neither bind to Hsp70 nor Hsp90 nor both are unable to rescue the defects of proteasomal assembly and protein degradation in Hop knockout cells [28]. We proposed that the Hsp70-Hop-Hsp90 ternary chaperone complex chaperones the docking of the 19S regulatory complex of the proteasome to the 20S core [28]. We further suggested that the Hsp70-Hop-Hsp90 ternary complex may contribute to stabilizing and maintaining the 26S/30S proteasome through transient regulatory interactions with some proteasomal subunits [28]. The molecular basis for the functions of the Hsp70-Hop-Hsp90 ternary complex in proteasome assembly and stability are not completely understood yet. Since the full atomic structure of the eukaryotic proteasome is available [145,146,147], experimental mapping of the Hsp70-Hop-Hsp90 ternary complex on the structures of proteasome subcomplexes or of the fully assembled mature proteasome by cryo-EM could be highly instructive.
In the context of the functions of the Hsp70-Hop-Hsp90 ternary chaperone complex in proteasomal assembly and function, there are several other open questions: (1) whether and how the ATP binding ability and ATPase activities of Hsp70 and Hsp90 are required; (2) whether other co-chaperones of the Hsp70 and/or Hsp90 chaperone systems are involved; (3) whether there are posttranslational modifications in any of the proteins of the ternary chaperone complex that may influence this process; (4) whether the Hsp70-Hop-Hsp90 complex collaborates with the canonical proteasome assembly chaperones to achieve the highest yield in the formation of the 26S/30S proteasome.
Hop in protein folding Surprisingly, protein folding appears to be even more efficient in the absence of Sti1/Hop in both yeast and human cancer cell lines [28] (Fig. 2b). The prokaryote-like binary complex consisting of Hsp70 and Hsp90 cannot only form in eukaryotic organisms in the absence of Hop, but this alternative chaperone complex is fully functional and ensures proteostasis [28]. As with the E. coli proteins, Hsp70, Hsp90, a JDP, a NEF, and ATP are the minimally required components for the in vitro refolding of the model substrate luciferase by the human Hop-independent alternative Hsp70–Hsp90 chaperone complex [28] (Fig. 2b). Increasing concentrations of Hop gradually decrease refolding of luciferase in vitro, and the direct interaction between Hsp70 and Hsp90 is essential for the refolding activity since mutating the interaction surface of Hsp90 reduces it [28]. The situation seems to be somewhat different with the components from budding yeast since the protein folding yield is positively influenced by Sti1 even though the Hsp70 and Hsp90 orthologs can also interact directly [108]. Several experimental differences may explain this apparent discrepancy: (1) As mentioned above, Sti1 and Hop affect the ATPase activity of their respective Hsp70 differently [95]; (2) different absolute or relative protein concentrations may have been used in these studies, which may be critical considering that different concentrations of Hsp70 can differentially impact the final yield of folded protein in these in vitro assays [4, 83].
While the Hsp70–Hsp90 binary complex, in the absence of Hop, is sufficient for folding and maturation of most Hsp90 clients in vivo, there are a few notable exceptions, including the glucocorticoid receptor (GR), v-Src, and, of course, the proteasome [28, 148]. Reduced levels of Hop have also been reported to affect the accumulation or stability of a few other Hsp90 clients, including LSD1 [149], GRK2 and Tau [126], TDP-43 [127], and MMP2 [150] in various cellular and mouse models. It could be hypothesized that Hop may influence client selectivity of Hsp90 in eukaryotic organisms [28, 148]. Intriguingly, for some Hsp90 clients, in vivo and in vitro systems yield differential protein folding and functional activities. For instance, the transfer of p53 from Hsp70 to Hsp90 and the folding to its native state require Hop in vitro [151, 152]. In contrast, in human cells, p53 protein levels are unaffected and its transcriptional activity is either the same or enhanced in the absence of Hop [28]. These discrepancies suggest that in the more complex intracellular milieu, the Hsp70–Hsp90 binary complex might be supported by other co-chaperones of the Hsp70 and Hsp90 systems, and/or yet other proteins (Fig. 2b). A genome-wide screen with the CRISPR-Cas9 system might reveal additional essential players responsible for the maximum chaperoning capacity of the Hsp70–Hsp90 binary complex. All of this evidence supports the conclusion that Hop is a novel regulator of proteostasis, whose levels and activity determine the balance between protein folding and degradation (Fig. 3).
Hop in cancer and neurodegenerative disorders
In cancer cells, Hsp90 is upregulated, and many oncogenic proteins (for example c-Abl, c-Raf, b-Raf, c-Src, Cdks, cyclins, mutant p53, Stat3, steroid receptors) rely on the chaperoning functions of Hsp90 for their folding, maturation, and activation [153,154,155,156]. This has led to the conclusion that Hsp90 is an oncogenic molecular chaperone [154, 155]. Similarly, Hsp70 is upregulated in cancer and favors oncogenesis [157, 158]. It has been reported that cancer types with more integrated and connected Hsp90-centered proteome hubs ("epichaperome"), of which Hop is an integral part, are more sensitive to Hsp90 inhibition than those with insular Hsp90 complexes [159, 160]. Consistent with the upregulation of both Hsp70 and Hsp90, Hop is also found to be overexpressed in several cancers at both the mRNA and protein levels [36,37,38,39, 161, 162]. These findings are reminiscent of how Hop was first discovered in human cells (see above) [19]. It was initially designated "transformation-sensitive human protein" IEF SSP 3621, before it was recognized that it is the human ortholog of the yeast protein Sti1 [19]. Although Hop is upregulated during oncogenesis, whether its overexpression is the consequence of cancer or whether it can initiate cancer, or, in other words, whether the Hop gene STIP1 is an oncogene still needs to be clarified.
Reducing Hop levels by RNA interference can revert cancer-specific phenotypes, including proliferation, invasion, migration, and metastasis [61, 150, 162, 163]; it reduces the levels of several Hsp90 clients, which correlates with the anticancer effects [162, 163]. While the outright knockout of Hop in human cancer cell lines, as mentioned above, minimally affects Hsp90 clients, it does further sensitize cancer cells to Hsp90 inhibitors [28]. Further studies are necessary to resolve these apparent differences between Hop knockout and knockdown models.
Unlike in cancer, all axes of the proteostatic network are reduced in some neurodegenerative disorders, including the levels of Hsp70 and Hsp90, as judged by their mRNA levels [164]. On the contrary, for Alzheimer's disease, the evidence suggests that the proteins Hsp70, Hsp90, and Hop are all upregulated [165,166,167]. Aggregation of certain pathological proteins correlates with the pathogenesis and prognosis in neurodegeneration. Many pathogenic proteins, which are thought to be responsible for neurodegenerative diseases, are Hsp90 clients or interactors, including tau (Alzheimer’s disease), α-synuclein (αSyn, Parkinson’s disease), and huntingtin (Htt, Huntington’s disease). A key question is whether it is the large insoluble aggregates or the soluble oligomers that are the most pathogenic. Although there is evidence in support of both hypotheses, most would agree today that it is the soluble oligomers, which are toxic to neurons [168, 169], and that therapeutic strategies should focus on them.
The chaperoning functions of Hsp70 are considered to be neuroprotective [164, 170], whereas the situation is confusing for Hsp90 and other components of the Hsp90 complex. Hsp90 has been proposed to inhibit the formation of oligomeric αSyn, further aggregation of which disrupts the mitochondrial membrane resulting in impaired mitochondrial function and the subsequent neuronal death in Parkinson’s disease [171]. For tau-toxicity in Alzheimer’s disease, Hsp90 has the opposite effect as it stabilizes the toxic soluble oligomers of tau in collaboration with its co-chaperones FKBP51/52 and Aha1; in this case, it prevents the formation of the cytoprotective larger aggregates [172,173,174]. The Hsp90 epichaperome was also proposed to be involved in the loss of dopaminergic neurons in the midbrain in Parkinson’s disease [175]. Therefore, directly manipulating the Hsp90 levels or activity might produce an opposite outcome depending on the underlying molecular mechanisms of a given neurodegenerative disease.
Relatively little is known about the functions of intracellular Hop in neurodegenerative diseases. Intracellular Hop in yeast protects cells from the toxicity of a mutant huntingtin with an aggregation-prone stretch of 103 glutamine residues (Htt103Q), and of the (PSI+) prion [176, 177]. In Caenorhabditis elegans, the knockdown of Hop aggravates the protein aggregation toxicity of amyloid β42 (Aβ42) without affecting lifespan [164]. In Drosophila melanogaster, loss of function of Hop enhances the toxicity of exogenous human tau [112]. Murine Hop, together with Hsp90, significantly reduces the aggregation of TDP-43, whose misfolding and formation of inclusions are hallmarks of amyotrophic lateral sclerosis [127]. Interestingly, as alluded to above, an age-dependent upregulation of Hop is evident in a mouse model of Alzheimer’s disease and in Alzheimer’s disease-affected human patients [167]. In all of these neurodegenerative disease models Hop seems to be protective, but several examples with opposing effects have emerged, too. A recent genetic screen in Drosophila melanogaster discovered beneficial effects of reduced levels of Hop. A Hop knockdown reduces the aggregation of Htt mutants with polyglutamine expansions and prevents their toxic phenotype [178]. Reminiscent of this finding, we recently demonstrated that the knockout of Hop in human cells reduces the aggregation of Htt72Q [28] (Fig. 3). The increased anti-aggregation activity of these cells requires Hsp90, since the chemical inhibition of Hsp90 reversed the phenomenon [28]. Intriguingly, the overexpression of Hop in a particular mouse model of Alzheimer’s disease has been reported to increase the accumulation of Aβ [179]. This evidence raises the possibility that molecular chaperones and their co-chaperones might only be neuroprotective within a narrow concentration range. Overall, the molecular and pathological functions of Hop in neurodegenerative disorders remain confusing and need to be explored more thoroughly using several more comparable disease-specific models. Manipulating the levels or activities of Hop might eventually be a promising avenue to treat both cancer and neurodegeneration.
Conclusions and perspectives
Studies on Hop have revealed its importance in fundamental cellular processes in both normal and pathophysiological conditions. Hop is differentially expressed in diseases like cancer and in aging, and it critically regulates activities of the Hsp70–Hsp90 chaperone system. Hop might, therefore, be an attractive target for future personalized medicine approaches.
Hsp90 inhibitors have been the subject of many clinical trials [154, 180] as anticancer therapies because cancer cells are more sensitive to such inhibitors than normal cells (discussed in [181]). The current knowledge, translational difficulties, and future perspectives of targeting Hsp90 in cancer have recently been reviewed [182,183,184]. Despite the initial setbacks of targeting Hsp90 in cancer, their addiction to Hsp90 may justify renewed efforts. These will include the more specific targeting of cancer-relevant isoforms of the Hsp90 family. Inhibiting Hsp90 co-chaperones rather than the whole Hsp90 chaperone machinery might also be worth exploring [28, 116, 185, 186]. Since ∆sti1 yeast mutants [113,114,115,116] and human Hop knockout cancer cells [28] are hypersensitive to Hsp90 inhibitors, combining Hop and Hsp90 inhibitors may be promising as cancer therapy. The inhibition of Hop would be expected to potentiate the effects of Hsp90 inhibitors, which could then be used at lower and even more cancer-specific doses. Those types of cancer that rely both on Hsp70–Hsp90-mediated protein folding and proteasome-mediated protein degradation may be particularly vulnerable since the inhibition of Hop reduces the proteasomal activity, which itself is an already established anti-cancer target. Some compounds have been demonstrated to inhibit the interaction between Hsp90 and the TPR2A domain of Hop [51, 187, 188]. The challenge will be to develop inhibitors that are as specific as possible for the interaction of Hop with either Hsp70 or Hsp90, without affecting the interactions of the latter with other TPR-containing co-chaperones.
Although Hsp90 function in neurodegenerative disorders remains paradoxical, as described above, activation of the chaperoning functions of the Hsp70–Hsp90 chaperone system could be therapeutically beneficial at least in some cases [164, 189]. Augmenting the activity of a protein or an enzyme is always challenging, but our recent findings revealed that the genetic removal of Hop strikingly enhances protein folding by the Hsp70–Hsp90 binary complex (Figs. 2b, 3) and reduces the aggregation of the pathological huntingtin protein [28] (Fig. 3). It remains to be seen whether non-toxic Hop inhibitors can be developed to boost Hsp70–Hsp90 chaperoning, notably in neuronal cells, and whether these inhibitors could be applied to certain neurodegenerative disorders.
Availability of data and material
Not applicable.
References
Mayer MP (2013) Hsp70 chaperone dynamics and molecular mechanism. Trends Biochem Sci 38(10):507–514. https://doi.org/10.1016/j.tibs.2013.08.001
Mayer MP, Bukau B (2005) Hsp70 chaperones: cellular functions and molecular mechanism. Cell Mol Life Sci 62(6):670–684. https://doi.org/10.1007/s00018-004-4464-6
Rüdiger S, Germeroth L, Schneider-Mergener J, Bukau B (1997) Substrate specificity of the DnaK chaperone determined by screening cellulose-bound peptide libraries. EMBO J 16(7):1501–1507. https://doi.org/10.1093/emboj/16.7.1501
Moran Luengo T, Mayer MP, Rüdiger SGD (2019) The Hsp70–Hsp90 chaperone cascade in protein folding. Trends Cell Biol 29(2):164–177. https://doi.org/10.1016/j.tcb.2018.10.004
Rosenzweig R, Nillegoda NB, Mayer MP, Bukau B (2019) The Hsp70 chaperone network. Nat Rev Mol Cell Biol 20(11):665–680. https://doi.org/10.1038/s41580-019-0133-3
Cuervo AM, Dice JF (1996) A receptor for the selective uptake and degradation of proteins by lysosomes. Science 273(5274):501–503. https://doi.org/10.1126/science.273.5274.501
Patel B, Cuervo AM (2015) Methods to study chaperone-mediated autophagy. Methods 75:133–140. https://doi.org/10.1016/j.ymeth.2015.01.003
Picard D (2002) Heat-shock protein 90, a chaperone for folding and regulation. Cell Mol Life Sci 59(10):1640–1648. https://doi.org/10.1007/pl00012491
Picard D (2012) Preface to Hsp90. Biochim Biophys Acta 1823(3):605–606. https://doi.org/10.1016/j.bbamcr.2012.02.004
Mayer MP, Le Breton L (2015) Hsp90: breaking the symmetry. Mol Cell 58(1):8–20. https://doi.org/10.1016/j.molcel.2015.02.022
Radli M, Rüdiger SGD (2018) Dancing with the Diva: Hsp90-client interactions. J Mol Biol 430(18):3029–3040. https://doi.org/10.1016/j.jmb.2018.05.026
Schopf FH, Biebl MM, Buchner J (2017) The HSP90 chaperone machinery. Nat Rev Mol Cell Biol 18(6):345–360. https://doi.org/10.1038/nrm.2017.20
Taipale M, Krykbaeva I, Koeva M, Kayatekin C, Westover KD, Karras GI, Lindquist S (2012) Quantitative analysis of HSP90-client interactions reveals principles of substrate recognition. Cell 150(5):987–1001. https://doi.org/10.1016/j.cell.2012.06.047
Echeverría PC (1803) Picard D (2010) Molecular chaperones, essential partners of steroid hormone receptors for activity and mobility. Biochim Biophys Acta 6:641–649. https://doi.org/10.1016/j.bbamcr.2009.11.012
Echeverría PC, Bernthaler A, Dupuis P, Mayer B, Picard D (2011) An interaction network predicted from public data as a discovery tool: application to the Hsp90 molecular chaperone machine. PLoS ONE 6(10):e26044. https://doi.org/10.1371/journal.pone.0026044
Scheufler C, Brinker A, Bourenkov G, Pegoraro S, Moroder L, Bartunik H, Hartl FU, Moarefi I (2000) Structure of TPR domain-peptide complexes: critical elements in the assembly of the Hsp70–Hsp90 multichaperone machine. Cell 101(2):199–210. https://doi.org/10.1016/S0092-8674(00)80830-2
Nicolet CM, Craig EA (1989) Isolation and characterization of STI1, a stress-inducible gene from Saccharomyces cerevisiae. Mol Cell Biol 9(9):3638–3646. https://doi.org/10.1128/mcb.9.9.3638
Tanguay RM (1988) Transcriptional activation of heat-shock genes in eukaryotes. Biochem Cell Biol 66(6):584–593. https://doi.org/10.1139/o88-069
Honoré B, Leffers H, Madsen P, Rasmussen HH, Vandekerckhove J, Celis JE (1992) Molecular cloning and expression of a transformation-sensitive human protein containing the TPR motif and sharing identity to the stress-inducible yeast protein STI1. J Biol Chem 267(12):8485–8491
Song S, Kole S, Precht P, Pazin MJ, Bernier M (2010) Activation of heat shock factor 1 plays a role in pyrrolidine dithiocarbamate-mediated expression of the co-chaperone BAG3. Int J Biochem Cell Biol 42(11):1856–1863. https://doi.org/10.1016/j.biocel.2010.07.021
Lee SD, Lai TW, Lin SZ, Lin CH, Hsu YH, Li CY, Wang HJ, Lee W, Su CY, Yu YL et al (2013) Role of stress-inducible protein-1 in recruitment of bone marrow derived cells into the ischemic brains. EMBO Mol Med 5(8):1227–1246. https://doi.org/10.1002/emmm.201202258
Mattison SA, Blatch GL, Edkins AL (2017) HOP expression is regulated by p53 and RAS and characteristic of a cancer gene signature. Cell Stress Chaperones 22(2):213–223. https://doi.org/10.1007/s12192-016-0755-8
Lassle M, Blatch GL, Kundra V, Takatori T, Zetter BR (1997) Stress-inducible, murine protein mSTI1. Characterization of binding domains for heat shock proteins and in vitro phosphorylation by different kinases. J Biol Chem 272(3):1876–1884. https://doi.org/10.1074/jbc.272.3.1876
Longshaw VM, Chapple JP, Balda MS, Cheetham ME, Blatch GL (2004) Nuclear translocation of the Hsp70/Hsp90 organizing protein mSTI1 is regulated by cell cycle kinases. J Cell Sci 117(Pt 5):701–710. https://doi.org/10.1242/jcs.00905
Daniel S, Bradley G, Longshaw VM, Soti C, Csermely P, Blatch GL (2008) Nuclear translocation of the phosphoprotein Hop (Hsp70/Hsp90 organizing protein) occurs under heat shock, and its proposed nuclear localization signal is involved in Hsp90 binding. Biochim Biophys Acta 1783(6):1003–1014. https://doi.org/10.1016/j.bbamcr.2008.01.014
Longshaw VM, Dirr HW, Blatch GL, Lassle M (2000) The in vitro phosphorylation of the co-chaperone mSTI1 by cell cycle kinases substantiates a predicted casein kinase II-p34cdc2-NLS (CcN) motif. Biol Chem 381(11):1133–1138. https://doi.org/10.1515/BC.2000.139
Yin H, Deng Z, Li X, Li Y, Yin W, Zhao G, Jiang D, Sun C, Zhou Y (2019) Down-regulation of STIP1 regulate apoptosis and invasion of glioma cells via TRAP1/AKT signaling pathway. Cancer Genet 237:1–9. https://doi.org/10.1016/j.cancergen.2019.05.006
Bhattacharya K, Weidenauer L, Luengo TM, Pieters EC, Echeverría PC, Bernasconi L, Wider D, Sadian Y, Koopman MB, Villemin M et al (2020) The Hsp70–Hsp90 co-chaperone Hop/Stip1 shifts the proteostatic balance from folding towards degradation. Nat Commun 11(1):5975. https://doi.org/10.1038/s41467-020-19783-w
Wang K, Jiang S, Huang A, Gao Y, Peng B, Li Z, Ma W, Songyang Z, Zhang S, He M et al (2020) GOLPH3 promotes cancer growth by interacting with STIP1 and regulating telomerase activity in pancreatic ductal adenocarcinoma. Front Oncol 10:575358. https://doi.org/10.3389/fonc.2020.575358
Soares IN, Caetano FA, Pinder J, Rodrigues BR, Beraldo FH, Ostapchenko VG, Durette C, Pereira GS, Lopes MH, Queiroz-Hazarbassanov N et al (2013) Regulation of stress-inducible phosphoprotein 1 nuclear retention by protein inhibitor of activated STAT PIAS1. Mol Cell Proteomics 12(11):3253–3270. https://doi.org/10.1074/mcp.M113.031005
Martins VR, Graner E, Garcia-Abreu J, de Souza SJ, Mercadante AF, Veiga SS, Zanata SM, Neto VM, Brentani RR (1997) Complementary hydropathy identifies a cellular prion protein receptor. Nat Med 3(12):1376–1382. https://doi.org/10.1038/nm1297-1376
Zanata SM, Lopes MH, Mercadante AF, Hajj GN, Chiarini LB, Nomizo R, Freitas AR, Cabral AL, Lee KS, Juliano MA et al (2002) Stress-inducible protein 1 is a cell surface ligand for cellular prion that triggers neuroprotection. EMBO J 21(13):3307–3316. https://doi.org/10.1093/emboj/cdf325
Lackie RE, Maciejewski A, Ostapchenko VG, Marques-Lopes J, Choy WY, Duennwald ML, Prado VF, Prado MAM (2017) The Hsp70/Hsp90 chaperone machinery in neurodegenerative diseases. Front Neurosci 11:254. https://doi.org/10.3389/fnins.2017.00254
Chiarini LB, Freitas AR, Zanata SM, Brentani RR, Martins VR, Linden R (2002) Cellular prion protein transduces neuroprotective signals. EMBO J 21(13):3317–3326. https://doi.org/10.1093/emboj/cdf324
Lima FR, Arantes CP, Muras AG, Nomizo R, Brentani RR, Martins VR (2007) Cellular prion protein expression in astrocytes modulates neuronal survival and differentiation. J Neurochem 103(6):2164–2176. https://doi.org/10.1111/j.1471-4159.2007.04904.x
Chen Z, Xu L, Su T, Ke Z, Peng Z, Zhang N, Peng S, Zhang Q, Liu G, Wei G et al (2017) Autocrine STIP1 signaling promotes tumor growth and is associated with disease outcome in hepatocellular carcinoma. Biochem Biophys Res Commun 493(1):365–372. https://doi.org/10.1016/j.bbrc.2017.09.016
Su T, Liao J, Dai Z, Xu L, Chen S, Wang Y, Peng Z, Zhang Q, Peng S, Kuang M (2018) Stress-induced phosphoprotein 1 mediates hepatocellular carcinoma metastasis after insufficient radiofrequency ablation. Oncogene 37(26):3514–3527. https://doi.org/10.1038/s41388-018-0169-4
Tsai CL, Tsai CN, Lin CY, Chen HW, Lee YS, Chao A, Wang TH, Wang HS, Lai CH (2012) Secreted stress-induced phosphoprotein 1 activates the ALK2-SMAD signaling pathways and promotes cell proliferation of ovarian cancer cells. Cell Rep 2(2):283–293. https://doi.org/10.1016/j.celrep.2012.07.002
Zhai E, Liang W, Lin Y, Huang L, He X, Cai S, Chen J, Zhang N, Li J, Zhang Q et al (2018) HSP70/HSP90-organizing protein contributes to gastric cancer progression in an autocrine fashion and predicts poor survival in gastric cancer. Cell Physiol Biochem 47(2):879–892. https://doi.org/10.1159/000490080
Erlich RB, Kahn SA, Lima FR, Muras AG, Martins RA, Linden R, Chiarini LB, Martins VR, Moura Neto V (2007) STI1 promotes glioma proliferation through MAPK and PI3K pathways. Glia 55(16):1690–1698. https://doi.org/10.1002/glia.20579
Baindur-Hudson S, Edkins AL, Blatch GL (2015) Hsp70/Hsp90 organising protein (hop): beyond interactions with chaperones and prion proteins. Subcell Biochem 78:69–90. https://doi.org/10.1007/978-3-319-11731-7_3
Hajj GN, Arantes CP, Dias MV, Roffe M, Costa-Silva B, Lopes MH, Porto-Carreiro I, Rabachini T, Lima FR, Beraldo FH et al (2013) The unconventional secretion of stress-inducible protein 1 by a heterogeneous population of extracellular vesicles. Cell Mol Life Sci 70(17):3211–3227. https://doi.org/10.1007/s00018-013-1328-y
Cruz L, Arevalo Romero JA, Brandao Prado M, Santos TG, Hohmuth Lopes M (2018) Evidence of extracellular vesicles biogenesis and release in mouse embryonic stem cells. Stem Cell Rev Rep 14(2):262–276. https://doi.org/10.1007/s12015-017-9776-7
Lauwers E, Wang YC, Gallardo R, Van der Kant R, Michiels E, Swerts J, Baatsen P, Zaiter SS, McAlpine SR, Gounko NV et al (2018) Hsp90 mediates membrane deformation and exosome release. Mol Cell 71(5):689–702. https://doi.org/10.1016/j.molcel.2018.07.016
da Fonseca ACC, Matias D, Geraldo LHM, Leser FS, Pagnoncelli I, Garcia C, do Amaral RF, da Rosa BG, Grimaldi I, de Camargo Magalhaes ES et al (2021) The multiple functions of the co-chaperone stress inducible protein 1. Cytokine Growth Factor Rev 57:73–84. https://doi.org/10.1016/j.cytogfr.2020.06.003
Smith DF, Sullivan WP, Marion TN, Zaitsu K, Madden B, McCormick DJ, Toft DO (1993) Identification of a 60-kilodalton stress-related protein, p60, which interacts with hsp90 and hsp70. Mol Cell Biol 13(2):869–876. https://doi.org/10.1128/mcb.13.2.869
Lamb JR, Tugendreich S, Hieter P (1995) Tetratrico peptide repeat interactions: to TPR or not to TPR? Trends Biochem Sci 20(7):257–259. https://doi.org/10.1016/s0968-0004(00)89037-4
Lee CT, Graf C, Mayer FJ, Richter SM, Mayer MP (2012) Dynamics of the regulation of Hsp90 by the co-chaperone Sti1. EMBO J 31(6):1518–1528. https://doi.org/10.1038/emboj.2012.37
Schmid AB, Lagleder S, Grawert MA, Rohl A, Hagn F, Wandinger SK, Cox MB, Demmer O, Richter K, Groll M et al (2012) The architecture of functional modules in the Hsp90 co-chaperone Sti1/Hop. EMBO J 31(6):1506–1517. https://doi.org/10.1038/emboj.2011.472
Kajander T, Sachs JN, Goldman A, Regan L (2009) Electrostatic interactions of Hsp-organizing protein tetratricopeptide domains with Hsp70 and Hsp90: computational analysis and protein engineering. J Biol Chem 284(37):25364–25374. https://doi.org/10.1074/jbc.M109.033894
Darby JF, Vidler LR, Simpson PJ, Al-Lazikani B, Matthews SJ, Sharp SY, Pearl LH, Hoelder S, Workman P (2020) Solution structure of the Hop TPR2A domain and investigation of target druggability by NMR, biochemical and in silico approaches. Sci Rep 10(1):16000. https://doi.org/10.1038/s41598-020-71969-w
Bose S, Weikl T, Bugl H, Buchner J (1996) Chaperone function of Hsp90-associated proteins. Science 274(5293):1715–1717. https://doi.org/10.1126/science.274.5293.1715
Prodromou C, Siligardi G, O’Brien R, Woolfson DN, Regan L, Panaretou B, Ladbury JE, Piper PW, Pearl LH (1999) Regulation of Hsp90 ATPase activity by tetratricopeptide repeat (TPR)-domain co-chaperones. EMBO J 18(3):754–762. https://doi.org/10.1093/emboj/18.3.754
Hildenbrand ZL, Molugu SK, Herrera N, Ramirez C, Xiao C , Bernal RA (2011) Hsp90 can accommodate the simultaneous binding of the FKBP52 and HOP proteins. Oncotarget 2(1–2):43–58. https://doi.org/10.18632/oncotarget.225
Onuoha SC, Coulstock ET, Grossmann JG, Jackson SE (2008) Structural studies on the co-chaperone Hop and its complexes with Hsp90. J Mol Biol 379(4):732–744. https://doi.org/10.1016/j.jmb.2008.02.013
Yi F, Doudevski I, Regan L (2010) HOP is a monomer: investigation of the oligomeric state of the co-chaperone HOP. Protein Sci 19(1):19–25. https://doi.org/10.1002/pro.278
Li J, Richter K, Buchner J (2011) Mixed Hsp90-cochaperone complexes are important for the progression of the reaction cycle. Nat Struct Mol Biol 18(1):61–66. https://doi.org/10.1038/nsmb.1965
Southworth DR, Agard DA (2011) Client-loading conformation of the Hsp90 molecular chaperone revealed in the cryo-EM structure of the human Hsp90: Hop complex. Mol Cell 42(6):771–781. https://doi.org/10.1016/j.molcel.2011.04.023
Beckley SJ, Hunter MC, Kituyi SN, Wingate I, Chakraborty A, Schwarz K, Makhubu MP, Rousseau RP, Ruck DK, de la Mare JA et al (2020) STIP1/HOP regulates the actin cytoskeleton through interactions with actin and changes in actin-binding proteins cofilin and profilin. Int J Mol Sci 21(9):3152. https://doi.org/10.3390/ijms21093152
Kituyi SN, Edkins AL (2018) Hop/STIP1 depletion alters nuclear structure via depletion of nuclear structural protein emerin. Biochem Biophys Res Commun 507(1–4):503–509. https://doi.org/10.1016/j.bbrc.2018.11.073
Li J, Sun X, Wang Z, Chen L, Li D, Zhou J, Liu M (2012) Regulation of vascular endothelial cell polarization and migration by Hsp70/Hsp90-organizing protein. PLoS ONE 7(4):e36389. https://doi.org/10.1371/journal.pone.0036389
Gangaraju VK, Yin H, Weiner MM, Wang J, Huang XA, Lin H (2011) Drosophila Piwi functions in Hsp90-mediated suppression of phenotypic variation. Nat Genet 43(2):153–158. https://doi.org/10.1038/ng.743
Abbas-Terki T, Briand PA, Donzé O, Picard D (2002) The Hsp90 co-chaperones Cdc37 and Sti1 interact physically and genetically. Biol Chem 383(9):1335–1342. https://doi.org/10.1515/BC.2002.152
Harst A, Lin H, Obermann WM (2005) Aha1 competes with Hop, p50 and p23 for binding to the molecular chaperone Hsp90 and contributes to kinase and hormone receptor activation. Biochem J 387(Pt 3):789–796. https://doi.org/10.1042/BJ20041283
Richter K, Muschler P, Hainzl O, Reinstein J, Buchner J (2003) Sti1 is a non-competitive inhibitor of the Hsp90 ATPase. Binding prevents the N-terminal dimerization reaction during the atpase cycle. J Biol Chem 278(12):10328–10333. https://doi.org/10.1074/jbc.M213094200
Johnson JL, Halas A, Flom G (2007) Nucleotide-dependent interaction of Saccharomyces cerevisiae Hsp90 with the cochaperone proteins Sti1, Cpr6, and Sba1. Mol Cell Biol 27(2):768–776. https://doi.org/10.1128/MCB.01034-06
Odunuga OO, Hornby JA, Bies C, Zimmermann R, Pugh DJ, Blatch GL (2003) Tetratricopeptide repeat motif-mediated Hsc70-mSTI1 interaction. Molecular characterization of the critical contacts for successful binding and specificity. J Biol Chem 278(9):6896–6904. https://doi.org/10.1074/jbc.M206867200
Rohl A, Wengler D, Madl T, Lagleder S, Tippel F, Herrmann M, Hendrix J, Richter K, Hack G, Schmid AB et al (2015) Hsp90 regulates the dynamics of its cochaperone Sti1 and the transfer of Hsp70 between modules. Nat Commun 6:6655. https://doi.org/10.1038/ncomms7655
Alvira S, Cuellar J, Rohl A, Yamamoto S, Itoh H, Alfonso C, Rivas G, Buchner J, Valpuesta JM (2014) Structural characterization of the substrate transfer mechanism in Hsp70/Hsp90 folding machinery mediated by Hop. Nat Commun 5:5484. https://doi.org/10.1038/ncomms6484
Hatherley R, Clitheroe CL, Faya N, Tastan Bishop O (2015) Plasmodium falciparum Hop: detailed analysis on complex formation with Hsp70 and Hsp90. Biochem Biophys Res Commun 456(1):440–445. https://doi.org/10.1016/j.bbrc.2014.11.103
Kirschke E, Goswami D, Southworth D, Griffin PR, Agard DA (2014) Glucocorticoid receptor function regulated by coordinated action of the Hsp90 and Hsp70 chaperone cycles. Cell 157(7):1685–1697. https://doi.org/10.1016/j.cell.2014.04.038
Brinker A, Scheufler C, Von Der Mulbe F, Fleckenstein B, Herrmann C, Jung G, Moarefi I, Hartl FU (2002) Ligand discrimination by TPR domains. Relevance and selectivity of EEVD-recognition in Hsp70 x Hop x Hsp90 complexes. J Biol Chem 277(22):19265–19275. https://doi.org/10.1074/jbc.M109002200
Bhattacharya K, Bernasconi L, Picard D (2018) Luminescence resonance energy transfer between genetically encoded donor and acceptor for protein-protein interaction studies in the molecular chaperone HSP70/HSP90 complexes. Sci Rep 8(1):2801. https://doi.org/10.1038/s41598-018-21210-6
Trcka F, Durech M, Man P, Hernychova L, Muller P, Vojtesek B (2014) The assembly and intermolecular properties of the Hsp70-Tomm34-Hsp90 molecular chaperone complex. J Biol Chem 289(14):9887–9901. https://doi.org/10.1074/jbc.M113.526046
Owens-Grillo JK, Czar MJ, Hutchison KA, Hoffmann K, Perdew GH, Pratt WB (1996) A model of protein targeting mediated by immunophilins and other proteins that bind to hsp90 via tetratricopeptide repeat domains. J Biol Chem 271(23):13468–13475. https://doi.org/10.1074/jbc.271.23.13468
Chang HC, Nathan DF, Lindquist S (1997) In vivo analysis of the Hsp90 cochaperone Sti1 (p60). Mol Cell Biol 17(1):318–325. https://doi.org/10.1128/mcb.17.1.318
Ebong IO, Beilsten-Edmands V, Patel NA, Morgner N, Robinson CV (2016) The interchange of immunophilins leads to parallel pathways and different intermediates in the assembly of Hsp90 glucocorticoid receptor complexes. Cell Discov 2:16002. https://doi.org/10.1038/celldisc.2016.2
Ebong IO, Morgner N, Zhou M, Saraiva MA, Daturpalli S, Jackson SE, Robinson CV (2011) Heterogeneity and dynamics in the assembly of the heat shock protein 90 chaperone complexes. Proc Natl Acad Sci USA 108(44):17939–17944. https://doi.org/10.1073/pnas.1106261108
Muller P, Ruckova E, Halada P, Coates PJ, Hrstka R, Lane DP, Vojtesek B (2013) C-terminal phosphorylation of Hsp70 and Hsp90 regulates alternate binding to co-chaperones CHIP and HOP to determine cellular protein folding/degradation balances. Oncogene 32(25):3101–3110. https://doi.org/10.1038/onc.2012.314
Szabo A, Langer T, Schroder H, Flanagan J, Bukau B, Hartl FU (1994) The ATP hydrolysis-dependent reaction cycle of the Escherichia coli Hsp70 system DnaK, DnaJ, and GrpE. Proc Natl Acad Sci USA 91(22):10345–10349. https://doi.org/10.1073/pnas.91.22.10345
Lewis MJ, Pelham HR (1985) Involvement of ATP in the nuclear and nucleolar functions of the 70 kd heat shock protein. EMBO J 4(12):3137–3143
Laufen T, Mayer MP, Beisel C, Klostermeier D, Mogk A, Reinstein J, Bukau B (1999) Mechanism of regulation of hsp70 chaperones by DnaJ cochaperones. Proc Natl Acad Sci USA 96(10):5452–5457. https://doi.org/10.1073/pnas.96.10.5452
Moran Luengo T, Kityk R, Mayer MP, Rüdiger SGD (2018) Hsp90 breaks the deadlock of the Hsp70 chaperone system. Mol Cell 70(3):545–552. https://doi.org/10.1016/j.molcel.2018.03.028
Li J, Soroka J, Buchner J (2012) The Hsp90 chaperone machinery: conformational dynamics and regulation by co-chaperones. Biochim Biophys Acta 1823(3):624–635. https://doi.org/10.1016/j.bbamcr.2011.09.003
Biebl MM, Buchner J (2019) Structure, function, and regulation of the Hsp90 machinery. Cold Spring Harb Perspect Biol 11(9):a034017. https://doi.org/10.1101/cshperspect.a034017
Li J, Richter K, Reinstein J, Buchner J (2013) Integration of the accelerator Aha1 in the Hsp90 co-chaperone cycle. Nat Struct Mol Biol 20(3):326–331. https://doi.org/10.1038/nsmb.2502
Ali MM, Roe SM, Vaughan CK, Meyer P, Panaretou B, Piper PW, Prodromou C, Pearl LH (2006) Crystal structure of an Hsp90-nucleotide-p23/Sba1 closed chaperone complex. Nature 440(7087):1013–1017. https://doi.org/10.1038/nature04716
Grad I, McKee TA, Ludwig SM, Hoyle GW, Ruiz P, Wurst W, Floss T, Miller CA 3rd, Picard D (2006) The Hsp90 cochaperone p23 is essential for perinatal survival. Mol Cell Biol 26(23):8976–8983. https://doi.org/10.1128/MCB.00734-06
Picard D (2006) Chaperoning steroid hormone action. Trends Endocrinol Metab 17(6):229–235. https://doi.org/10.1016/j.tem.2006.06.003
Freeman BC, Felts SJ, Toft DO, Yamamoto KR (2000) The p23 molecular chaperones act at a late step in intracellular receptor action to differentially affect ligand efficacies. Genes Dev 14(4):422–434
Rehn AB, Buchner J (2015) p23 and Aha1. Subcell Biochem 78:113–131. https://doi.org/10.1007/978-3-319-11731-7_6
Gaiser AM, Brandt F, Richter K (2009) The non-canonical Hop protein from Caenorhabditis elegans exerts essential functions and forms binary complexes with either Hsc70 or Hsp90. J Mol Biol 391(3):621–634. https://doi.org/10.1016/j.jmb.2009.06.051
Song HO, Lee W, An K, Lee HS, Cho JH, Park ZY, Ahnn J (2009) C. elegans STI-1, the homolog of Sti1/Hop, is involved in aging and stress response. J Mol Biol 390(4):604–617. https://doi.org/10.1016/j.jmb.2009.05.035
Kaziales A, Barkovits K, Marcus K, Richter K (2020) Glucocorticoid receptor complexes form cooperatively with the Hsp90 co-chaperones Pp5 and FKBPs. Sci Rep 10(1):10733. https://doi.org/10.1038/s41598-020-67645-8
Wegele H, Haslbeck M, Reinstein J, Buchner J (2003) Sti1 is a novel activator of the Ssa proteins. J Biol Chem 278(28):25970–25976. https://doi.org/10.1074/jbc.M301548200
Reidy M, Kumar S, Anderson DE, Masison DC (2018) Dual roles for yeast Sti1/Hop in regulating the hsp90 chaperone cycle. Genetics 209(4):1139–1154. https://doi.org/10.1534/genetics.118.301178
Rohl A, Tippel F, Bender E, Schmid AB, Richter K, Madl T , Buchner J (2015) Hop/Sti1 phosphorylation inhibits its co-chaperone function. EMBO Rep 16(2):240–249. https://doi.org/10.15252/embr.201439198
Schreiber TB, Mausbacher N, Soroka J, Wandinger SK, Buchner J, Daub H (2012) Global analysis of phosphoproteome regulation by the Ser/Thr phosphatase Ppt1 in Saccharomyces cerevisiae. J Proteome Res 11(4):2397–2408. https://doi.org/10.1021/pr201134p
Assenza S, Sassi AS, Kellner R, Schuler B, De Los RP, Barducci A (2019) Efficient conversion of chemical energy into mechanical work by Hsp70 chaperones. Elife 8:e48491. https://doi.org/10.7554/eLife.48491
Yamamoto S, Subedi GP, Hanashima S, Satoh T, Otaka M, Wakui H, Sawada K, Yokota S, Yamaguchi Y, Kubota H et al (2014) ATPase activity and ATP-dependent conformational change in the co-chaperone HSP70/HSP90-organizing protein (HOP). J Biol Chem 289(14):9880–9886. https://doi.org/10.1074/jbc.M114.553255
Freeman BC, Toft DO, Morimoto RI (1996) Molecular chaperone machines: chaperone activities of the cyclophilin Cyp-40 and the steroid aporeceptor-associated protein p23. Science 274(5293):1718–1720. https://doi.org/10.1126/science.274.5293.1718
Hernandez MP, Sullivan WP, Toft DO (2002) The assembly and intermolecular properties of the hsp70-Hop-hsp90 molecular chaperone complex. J Biol Chem 277(41):38294–38304. https://doi.org/10.1074/jbc.M206566200
Genest O, Hoskins JR, Camberg JL, Doyle SM, Wickner S (2011) Heat shock protein 90 from Escherichia coli collaborates with the DnaK chaperone system in client protein remodeling. Proc Natl Acad Sci USA 108(20):8206–8211. https://doi.org/10.1073/pnas.1104703108
Genest O, Hoskins JR, Kravats AN, Doyle SM, Wickner S (2015) Hsp70 and Hsp90 of E. coli directly interact for collaboration in protein remodeling. J Mol Biol 427(24):3877–3889. https://doi.org/10.1016/j.jmb.2015.10.010
Kravats AN, Doyle SM, Hoskins JR, Genest O, Doody E, Wickner S (2017) Interaction of E. coli Hsp90 with DnaK involves the DnaJ binding region of DnaK. J Mol Biol 429(6):858–872. https://doi.org/10.1016/j.jmb.2016.12.014
Nakamoto H, Fujita K, Ohtaki A, Watanabe S, Narumi S, Maruyama T, Suenaga E, Misono TS, Kumar PK, Goloubinoff P et al (2014) Physical interaction between bacterial heat shock protein (Hsp) 90 and Hsp70 chaperones mediates their cooperative action to refold denatured proteins. J Biol Chem 289(9):6110–6119. https://doi.org/10.1074/jbc.M113.524801
Grindle MP, Carter B, Alao JP, Connors K, Tehver R, Kravats AN (2021) Structural communication between the E. coli chaperones DnaK and Hsp90. Int J Mol Sci 22(4):2200. https://doi.org/10.3390/ijms22042200
Kravats AN, Hoskins JR, Reidy M, Johnson JL, Doyle SM, Genest O, Masison DC, Wickner S (2018) Functional and physical interaction between yeast Hsp90 and Hsp70. Proc Natl Acad Sci USA 115(10):E2210–E2219. https://doi.org/10.1073/pnas.1719969115
Sung N, Lee J, Kim JH, Chang C, Joachimiak A, Lee S, Tsai FT (2016) Mitochondrial Hsp90 is a ligand-activated molecular chaperone coupling ATP binding to dimer closure through a coiled-coil intermediate. Proc Natl Acad Sci USA 113(11):2952–2957. https://doi.org/10.1073/pnas.1516167113
Sun M, Kotler JLM, Liu S, Street TO (2019) The endoplasmic reticulum (ER) chaperones BiP and Grp94 selectively associate when BiP is in the ADP conformation. J Biol Chem 294(16):6387–6396. https://doi.org/10.1074/jbc.RA118.007050
Joshi A, Dai L, Liu Y, Lee J, Ghahhari NM, Segala G, Beebe K, Jenkins LM, Lyons GC, Bernasconi L et al (2020) The mitochondrial HSP90 paralog TRAP1 forms an OXPHOS-regulated tetramer and is involved in mitochondrial metabolic homeostasis. BMC Biol 18(1):10. https://doi.org/10.1186/s12915-020-0740-7
Ambegaokar SS, Jackson GR (2011) Functional genomic screen and network analysis reveal novel modifiers of tauopathy dissociated from tau phosphorylation. Hum Mol Genet 20(24):4947–4977. https://doi.org/10.1093/hmg/ddr432
Parsons AB, Lopez A, Givoni IE, Williams DE, Gray CA, Porter J, Chua G, Sopko R, Brost RL, Ho CH et al (2006) Exploring the mode-of-action of bioactive compounds by chemical-genetic profiling in yeast. Cell 126(3):611–625. https://doi.org/10.1016/j.cell.2006.06.040
Zhao R, Davey M, Hsu YC, Kaplanek P, Tong A, Parsons AB, Krogan N, Cagney G, Mai D, Greenblatt J et al (2005) Navigating the chaperone network: an integrative map of physical and genetic interactions mediated by the hsp90 chaperone. Cell 120(5):715–727. https://doi.org/10.1016/j.cell.2004.12.024
Piper PW, Millson SH, Mollapour M, Panaretou B, Siligardi G, Pearl LH, Prodromou C (2003) Sensitivity to Hsp90-targeting drugs can arise with mutation to the Hsp90 chaperone, cochaperones and plasma membrane ATP binding cassette transporters of yeast. Eur J Biochem 270(23):4689–4695. https://doi.org/10.1046/j.1432-1033.2003.03866.x
Liu XD, Morano KA, Thiele DJ (1999) The yeast Hsp110 family member, Sse1, is an Hsp90 cochaperone. J Biol Chem 274(38):26654–26660. https://doi.org/10.1074/jbc.274.38.26654
Millson SH, Nuttall JM, Mollapour M, Piper PW (2009) The Hsp90/Cdc37p chaperone system is a determinant of molybdate resistance in Saccharomyces cerevisiae. Yeast 26(6):339–347. https://doi.org/10.1002/yea.1670
Duina AA, Chang HC, Marsh JA, Lindquist S, Gaber RF (1996) A cyclophilin function in Hsp90-dependent signal transduction. Science 274(5293):1713–1715. https://doi.org/10.1126/science.274.5293.1713
Jones MH, Bachant JB, Castillo AR, Giddings TH Jr, Winey M (1999) Yeast Dam1p is required to maintain spindle integrity during mitosis and interacts with the Mps1p kinase. Mol Biol Cell 10(7):2377–2391. https://doi.org/10.1091/mbc.10.7.2377
Caplan AJ, Ma’ayan A, Willis IM (2007) Multiple kinases and system robustness: a link between Cdc37 and genome integrity. Cell Cycle 6(24):3145–3147. https://doi.org/10.4161/cc.6.24.5147
Fang Y, Fliss AE, Rao J, Caplan AJ (1998) SBA1 encodes a yeast hsp90 cochaperone that is homologous to vertebrate p23 proteins. Mol Cell Biol 18(7):3727–3734. https://doi.org/10.1128/mcb.18.7.3727
Flom G, Weekes J, Johnson JL (2005) Novel interaction of the Hsp90 chaperone machine with Ssl2, an essential DNA helicase in Saccharomyces cerevisiae. Curr Genet 47(6):368–380. https://doi.org/10.1007/s00294-005-0580-6
Kudze T, Mendez-Dorantes C, Jalloh CS, McClellan AJ (2018) Evidence for interaction between Hsp90 and the ER membrane complex. Cell Stress Chaperones 23(5):1101–1115. https://doi.org/10.1007/s12192-018-0908-z
Chakraborty A, Edkins AL (2020) Hop depletion reduces HSF1 levels and activity and coincides with reduced stress resilience. Biochem Biophys Res Commun 527(2):440–446. https://doi.org/10.1016/j.bbrc.2020.04.072
Beraldo FH, Soares IN, Goncalves DF, Fan J, Thomas AA, Santos TG, Mohammad AH, Roffe M, Calder MD, Nikolova S et al (2013) Stress-inducible phosphoprotein 1 has unique cochaperone activity during development and regulates cellular response to ischemia via the prion protein. FASEB J 27(9):3594–3607. https://doi.org/10.1096/fj.13-232280
Lackie RE, Razzaq AR, Farhan SMK, Qiu LR, Moshitzky G, Beraldo FH, Lopes MH, Maciejewski A, Gros R, Fan J et al (2020) Modulation of hippocampal neuronal resilience during aging by the Hsp70/Hsp90 co-chaperone STI1. J Neurochem 153(6):727–758. https://doi.org/10.1111/jnc.14882
Lin LT, Razzaq A, Di Gregorio SE, Hong S, Charles B, Lopes MH, Beraldo F, Prado VF, Prado MAM, Duennwald ML (2021) Hsp90 and its co-chaperone Sti1 control TDP-43 misfolding and toxicity. FASEB J 35(5):e21594. https://doi.org/10.1096/fj.202002645R
Hombach A, Ommen G, Chrobak M, Clos J (2013) The Hsp90-Sti1 interaction is critical for Leishmania donovani proliferation in both life cycle stages. Cell Microbiol 15(4):585–600. https://doi.org/10.1111/cmi.12057
Hipp MS, Park SH, Hartl FU (2014) Proteostasis impairment in protein-misfolding and -aggregation diseases. Trends Cell Biol 24(9):506–514. https://doi.org/10.1016/j.tcb.2014.05.003
Wong E, Cuervo AM (2010) Integration of clearance mechanisms: the proteasome and autophagy. Cold Spring Harb Perspect Biol 2(12):a006734. https://doi.org/10.1101/cshperspect.a006734
Chen B, Retzlaff M, Roos T, Frydman J (2011) Cellular strategies of protein quality control. Cold Spring Harb Perspect Biol 3(8):a004374. https://doi.org/10.1101/cshperspect.a004374
Balchin D, Hayer-Hartl M, Hartl FU (2016) In vivo aspects of protein folding and quality control. Science 353(6294):aac4354. https://doi.org/10.1126/science.aac4354
Shalgi R, Hurt JA, Krykbaeva I, Taipale M, Lindquist S, Burge CB (2013) Widespread regulation of translation by elongation pausing in heat shock. Mol Cell 49(3):439–452. https://doi.org/10.1016/j.molcel.2012.11.028
Kakihara Y, Houry WA (2012) The R2TP complex: discovery and functions. Biochim Biophys Acta 1823(1):101–107. https://doi.org/10.1016/j.bbamcr.2011.08.016
Schopf FH, Huber EM, Dodt C, Lopez A, Biebl MM, Rutz DA, Muhlhofer M, Richter G, Madl T, Sattler M et al (2019) The co-chaperone Cns1 and the recruiter protein Hgh1 link Hsp90 to translation elongation via chaperoning elongation factor 2. Mol Cell 74(1):73–87. https://doi.org/10.1016/j.molcel.2019.02.011
Boulon S, Bertrand E, Pradet-Balade B (2012) HSP90 and the R2TP co-chaperone complex: building multi-protein machineries essential for cell growth and gene expression. RNA Biol 9(2):148–154. https://doi.org/10.4161/rna.18494
Willmund F, del Alamo M, Pechmann S, Chen T, Albanese V, Dammer EB, Peng J, Frydman J (2013) The cotranslational function of ribosome-associated Hsp70 in eukaryotic protein homeostasis. Cell 152(1–2):196–209. https://doi.org/10.1016/j.cell.2012.12.001
Geller R, Pechmann S, Acevedo A, Andino R, Frydman J (2018) Hsp90 shapes protein and RNA evolution to balance trade-offs between protein stability and aggregation. Nat Commun 9(1):1781. https://doi.org/10.1038/s41467-018-04203-x
Wiech H, Buchner J, Zimmermann R, Jakob U (1992) Hsp90 chaperones protein folding in vitro. Nature 358(6382):169–170. https://doi.org/10.1038/358169a0
Balasubramanian A, Markovski M, Hoskins JR, Doyle SM, Wickner S (2019) Hsp90 of E. coli modulates assembly of FtsZ, the bacterial tubulin homolog. Proc Natl Acad Sci USA 116(25):12285–12294. https://doi.org/10.1073/pnas.1904014116
Kaushik S, Cuervo AM (2018) The coming of age of chaperone-mediated autophagy. Nat Rev Mol Cell Biol 19(6):365–381. https://doi.org/10.1038/s41580-018-0001-6
Imai J, Maruya M, Yashiroda H, Yahara I, Tanaka K (2003) The molecular chaperone Hsp90 plays a role in the assembly and maintenance of the 26S proteasome. EMBO J 22(14):3557–3567. https://doi.org/10.1093/emboj/cdg349
Lander GC, Estrin E, Matyskiela ME, Bashore C, Nogales E, Martin A (2012) Complete subunit architecture of the proteasome regulatory particle. Nature 482(7384):186–191. https://doi.org/10.1038/nature10774
Sha L, Wang X, Li J, Shi X, Wu L, Shen Y, Xu Q (2017) Pharmacologic inhibition of Hsp90 to prevent GLT-1 degradation as an effective therapy for epilepsy. J Exp Med 214(2):547–563. https://doi.org/10.1084/jem.20160667
Tanaka K (2009) The proteasome: overview of structure and functions. Proc Jpn Acad Ser B Phys Biol Sci 85(1):12–36. https://doi.org/10.2183/pjab.85.12
Huang X, Luan B, Wu J, Shi Y (2016) An atomic structure of the human 26S proteasome. Nat Struct Mol Biol 23(9):778–785. https://doi.org/10.1038/nsmb.3273
Schweitzer A, Aufderheide A, Rudack T, Beck F, Pfeifer G, Plitzko JM, Sakata E, Schulten K, Forster F, Baumeister W (2016) Structure of the human 26S proteasome at a resolution of 3.9 Å. Proc Natl Acad Sci USA 113(28):7816–7821. https://doi.org/10.1073/pnas.1608050113
Sahasrabudhe P, Rohrberg J, Biebl MM, Rutz DA, Buchner J (2017) The plasticity of the Hsp90 co-chaperone system. Mol Cell 67(6):947–961. https://doi.org/10.1016/j.molcel.2017.08.004
Tsai CL, Chao AS, Jung SM, Lin CY, Chao A, Wang TH (2018) Stress-induced phosphoprotein 1 acts as a scaffold protein for glycogen synthase kinase-3 β-mediated phosphorylation of lysine-specific demethylase 1. Oncogenesis 7(3):31. https://doi.org/10.1038/s41389-018-0040-z
Walsh N, Larkin A, Swan N, Conlon K, Dowling P, McDermott R, Clynes M (2011) RNAi knockdown of Hop (Hsp70/Hsp90 organising protein) decreases invasion via MMP-2 down regulation. Cancer Lett 306(2):180–189. https://doi.org/10.1016/j.canlet.2011.03.004
Dahiya V, Agam G, Lawatscheck J, Rutz DA, Lamb DC, Buchner J (2019) Coordinated conformational processing of the tumor suppressor protein p53 by the Hsp70 and Hsp90 chaperone machineries. Mol Cell 74(4):816–830. https://doi.org/10.1016/j.molcel.2019.03.026
Boysen M, Kityk R, Mayer MP (2019) Hsp70- and Hsp90-mediated regulation of the conformation of p53 DNA binding domain and p53 cancer variants. Mol Cell 74(4):831–843. https://doi.org/10.1016/j.molcel.2019.03.032
Mahalingam D, Swords R, Carew JS, Nawrocki ST, Bhalla K, Giles FJ (2009) Targeting HSP90 for cancer therapy. Br J Cancer 100(10):1523–1529. https://doi.org/10.1038/sj.bjc.6605066
Trepel J, Mollapour M, Giaccone G, Neckers L (2010) Targeting the dynamic HSP90 complex in cancer. Nat Rev Cancer 10(8):537–549. https://doi.org/10.1038/nrc2887
Miyata Y, Nakamoto H, Neckers L (2013) The therapeutic target Hsp90 and cancer hallmarks. Curr Pharm Des 19(3):347–365. https://doi.org/10.2174/138161213804143725
Vartholomaiou E, Echeverría PC, Picard D (2016) Unusual suspects in the twilight zone between the Hsp90 interactome and carcinogenesis. Adv Cancer Res 129:1–30. https://doi.org/10.1016/bs.acr.2015.08.001
Murphy ME (2013) The HSP70 family and cancer. Carcinogenesis 34(6):1181–1188. https://doi.org/10.1093/carcin/bgt111
Sherman MY, Gabai VL (2015) Hsp70 in cancer: back to the future. Oncogene 34(32):4153–4161. https://doi.org/10.1038/onc.2014.349
Rodina A, Wang T, Yan P, Gomes ED, Dunphy MP, Pillarsetty N, Koren J, Gerecitano JF, Taldone T, Zong H et al (2016) The epichaperome is an integrated chaperome network that facilitates tumour survival. Nature 538(7625):397–401. https://doi.org/10.1038/nature19807
Joshi S, Wang T, Araujo TLS, Sharma S, Brodsky JL, Chiosis G (2018) Adapting to stress - chaperome networks in cancer. Nat Rev Cancer 18(9):562–575. https://doi.org/10.1038/s41568-018-0020-9
Kubota H, Yamamoto S, Itoh E, Abe Y, Nakamura A, Izumi Y, Okada H, Iida M, Nanjo H, Itoh H et al (2010) Increased expression of co-chaperone HOP with HSP90 and HSC70 and complex formation in human colonic carcinoma. Cell Stress Chaperones 15(6):1003–1011. https://doi.org/10.1007/s12192-010-0211-0
Jing Y, Liang W, Liu J, Zhang L, Wei J, Zhu Y, Yang J, Ji K, Zhang Y, Huang Z (2019) Stress-induced phosphoprotein 1 promotes pancreatic cancer progression through activation of the FAK/AKT/MMP signaling axis. Pathol Res Pract 215(11):152564. https://doi.org/10.1016/j.prp.2019.152564
Willmer T, Contu L, Blatch GL, Edkins AL (2013) Knockdown of Hop downregulates RhoC expression, and decreases pseudopodia formation and migration in cancer cell lines. Cancer Lett 328(2):252–260. https://doi.org/10.1016/j.canlet.2012.09.021
Brehme M, Voisine C, Rolland T, Wachi S, Soper JH, Zhu Y, Orton K, Villella A, Garza D, Vidal M et al (2014) A chaperome subnetwork safeguards proteostasis in aging and neurodegenerative disease. Cell Rep 9(3):1135–1150. https://doi.org/10.1016/j.celrep.2014.09.042
Perez N, Sugar J, Charya S, Johnson G, Merril C, Bierer L, Perl D, Haroutunian V, Wallace W (1991) Increased synthesis and accumulation of heat shock 70 proteins in Alzheimer’s disease. Brain Res Mol Brain Res 11(3–4):249–254. https://doi.org/10.1016/0169-328x(91)90033-t
Kakimura J, Kitamura Y, Takata K, Umeki M, Suzuki S, Shibagaki K, Taniguchi T, Nomura Y, Gebicke-Haerter PJ, Smith MA et al (2002) Microglial activation and amyloid-beta clearance induced by exogenous heat-shock proteins. FASEB J 16(6):601–603. https://doi.org/10.1096/fj.01-0530fje
Ostapchenko VG, Beraldo FH, Mohammad AH, Xie YF, Hirata PH, Magalhaes AC, Lamour G, Li H, Maciejewski A, Belrose JC et al (2013) The prion protein ligand, stress-inducible phosphoprotein 1, regulates amyloid-beta oligomer toxicity. J Neurosci 33(42):16552–16564. https://doi.org/10.1523/JNEUROSCI.3214-13.2013
Haass C, Selkoe DJ (2007) Soluble protein oligomers in neurodegeneration: lessons from the Alzheimer’s amyloid β-peptide. Nat Rev Mol Cell Biol 8(2):101–112. https://doi.org/10.1038/nrm2101
Ferreira ST, Vieira MN, De Felice FG (2007) Soluble protein oligomers as emerging toxins in Alzheimer’s and other amyloid diseases. IUBMB Life 59(4–5):332–345. https://doi.org/10.1080/15216540701283882
Hartl FU, Hayer-Hartl M (2009) Converging concepts of protein folding in vitro and in vivo. Nat Struct Mol Biol 16(6):574–581. https://doi.org/10.1038/nsmb.1591
Burmann BM, Gerez JA, Matecko-Burmann I, Campioni S, Kumari P, Ghosh D, Mazur A, Aspholm EE, Sulskis D, Wawrzyniuk M et al (2020) Regulation of α-synuclein by chaperones in mammalian cells. Nature 577(7788):127–132. https://doi.org/10.1038/s41586-019-1808-9
Weickert S, Wawrzyniuk M, John LH, Rüdiger SGD, Drescher M (2020) The mechanism of Hsp90-induced oligomerizaton of Tau. Sci Adv 6(11):aax6999. https://doi.org/10.1126/sciadv.aax6999
Blair LJ, Nordhues BA, Hill SE, Scaglione KM, O’Leary JC 3rd, Fontaine SN, Breydo L, Zhang B, Li P, Wang L et al (2013) Accelerated neurodegeneration through chaperone-mediated oligomerization of tau. J Clin Invest 123(10):4158–4169. https://doi.org/10.1172/JCI69003
Criado-Marrero M, Gebru NT, Blazier DM, Gould LA, Baker JD, Beaulieu-Abdelahad D, Blair LJ (2021) Hsp90 co-chaperones, FKBP52 and Aha1, promote tau pathogenesis in aged wild-type mice. Acta Neuropathol Commun 9(1):65. https://doi.org/10.1186/s40478-021-01159-w
Kishinevsky S, Wang T, Rodina A, Chung SY, Xu C, Philip J, Taldone T, Joshi S, Alpaugh ML, Bolaender A et al (2018) HSP90-incorporating chaperome networks as biosensor for disease-related pathways in patient-specific midbrain dopamine neurons. Nat Commun 9(1):4345. https://doi.org/10.1038/s41467-018-06486-6
Wolfe KJ, Ren HY, Trepte P, Cyr DM (2013) The Hsp70/90 cochaperone, Sti1, suppresses proteotoxicity by regulating spatial quality control of amyloid-like proteins. Mol Biol Cell 24(23):3588–3602. https://doi.org/10.1091/mbc.E13-06-0315
Reidy M, Masison DC (2010) Sti1 regulation of Hsp70 and Hsp90 is critical for curing of Saccharomyces cerevisiae [PSI+] prions by Hsp104. Mol Cell Biol 30(14):3542–3552. https://doi.org/10.1128/MCB.01292-09
Xu F, Kula-Eversole E, Iwanaszko M, Hutchison AL, Dinner A, Allada R (2019) Circadian clocks function in concert with heat shock organizing protein to modulate mutant huntingtin aggregation and toxicity. Cell Rep 27(1):59–70. https://doi.org/10.1016/j.celrep.2019.03.015
Lackie RE, Marques-Lopes J, Ostapchenko VG, Good S, Choy WY, van Oosten-Hawle P, Pasternak SH, Prado VF, Prado MAM (2020) Increased levels of Stress-inducible phosphoprotein-1 accelerates amyloid-β deposition in a mouse model of Alzheimer’s disease. Acta Neuropathol Commun 8(1):143. https://doi.org/10.1186/s40478-020-01013-5
Neckers L, Workman P (2012) Hsp90 molecular chaperone inhibitors: are we there yet? Clin Cancer Res 18(1):64–76. https://doi.org/10.1158/1078-0432.CCR-11-1000
Echeverría PC, Bhattacharya K, Joshi A, Wang T, Picard D (2019) The sensitivity to Hsp90 inhibitors of both normal and oncogenically transformed cells is determined by the equilibrium between cellular quiescence and activity. PLoS ONE 14(2):e0208287. https://doi.org/10.1371/journal.pone.0208287
Neckers L, Blagg B, Haystead T, Trepel JB, Whitesell L, Picard D (2018) Methods to validate Hsp90 inhibitor specificity, to identify off-target effects, and to rethink approaches for further clinical development. Cell Stress Chaperones 23(4):467–482. https://doi.org/10.1007/s12192-018-0877-2
Graner MW (2021) Making HSP90 inhibitors great again? unite for better cancer immunotherapy. Cell Chem Biol 28(2):118–120. https://doi.org/10.1016/j.chembiol.2021.02.002
Banerjee M, Hatial I, Keegan BM, Blagg BSJ (2021) Assay design and development strategies for finding Hsp90 inhibitors and their role in human diseases. Pharmacol Ther 221:107747. https://doi.org/10.1016/j.pharmthera.2020.107747
Forafonov F, Toogun OA, Grad I, Suslova E, Freeman BC, Picard D (2008) p23/Sba1p protects against Hsp90 inhibitors independently of its intrinsic chaperone activity. Mol Cell Biol 28(10):3446–3456. https://doi.org/10.1128/MCB.02246-07
Holmes JL, Sharp SY, Hobbs S, Workman P (2008) Silencing of HSP90 cochaperone AHA1 expression decreases client protein activation and increases cellular sensitivity to the HSP90 inhibitor 17-allylamino-17-demethoxygeldanamycin. Cancer Res 68(4):1188–1197. https://doi.org/10.1158/0008-5472.CAN-07-3268
Yi F, Regan L (2008) A novel class of small molecule inhibitors of Hsp90. ACS Chem Biol 3(10):645–654. https://doi.org/10.1021/cb800162x
Pimienta G, Herbert KM, Regan L (2011) A compound that inhibits the HOP-Hsp90 complex formation and has unique killing effects in breast cancer cell lines. Mol Pharm 8(6):2252–2261. https://doi.org/10.1021/mp200346y
Labbadia J, Morimoto RI (2015) The biology of proteostasis in aging and disease. Annu Rev Biochem 84:435–464. https://doi.org/10.1146/annurev-biochem-060614-033955
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KB and DP conceived the theme, reviewed the literature, and wrote the manuscript. KB is a senior collaborator in the laboratory of DP who is a professor of cell biology.
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Bhattacharya, K., Picard, D. The Hsp70–Hsp90 go-between Hop/Stip1/Sti1 is a proteostatic switch and may be a drug target in cancer and neurodegeneration. Cell. Mol. Life Sci. 78, 7257–7273 (2021). https://doi.org/10.1007/s00018-021-03962-z
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DOI: https://doi.org/10.1007/s00018-021-03962-z