Preservation and Storage of Cells for Therapy: Fundamental Aspects of Low Temperature Science

Buriak, Iryna A.; Elliott, Gloria; Fleck, Roland A.; Fuller, Barry J.; Glasmacher, Birgit; Goltsev, Anatoliy M.; Gryshkov, Oleksandr; Guibert, Edgardo E.; Hagedorn, Janina; Juan de Paz, Leonardo; Mutsenko, Vitalii; Petrenko, Alexander Yu.; Petrenko, Yuriy; Rittinghaus, Tim; Robert, María Celeste; Leal-Marin, Sara; Stacey, Glyn Nigel; Trufanova, Natalia A.

doi:10.1007/978-3-319-37076-7_67-1

Iryna A. Buriak⁶,
Gloria Elliott⁷,
Roland A. Fleck⁸,
Barry J. Fuller⁹,
Birgit Glasmacher¹⁰,
Anatoliy M. Goltsev⁶,
Oleksandr Gryshkov¹⁰,
Edgardo E. Guibert¹¹,
Janina Hagedorn¹⁰,
Leonardo Juan de Paz¹¹,
Vitalii Mutsenko¹⁰,
Alexander Yu. Petrenko^6,12,
Yuriy Petrenko¹³,
Tim Rittinghaus¹⁰,
María Celeste Robert¹¹,
Sara Leal-Marin¹⁰,
Glyn Nigel Stacey^14,15,16 &
…
Natalia A. Trufanova⁶

Part of the book series: Reference Series in Biomedical Engineering ((TIENRE))

85 Accesses

Abstract

Preservation and storage of living cells play an important role in many areas of biotechnology and cell therapy. In most cases the power of cold to control biological deterioration forms the basis of the preservation strategies, but low temperatures of themselves can be equally disruptive on cell function if applied in inappropriate ways. Successful preservation protocols have developed to mitigate these detrimental effects. In this chapter, we discuss how low temperatures coupled with the use of cryoprotectants are currently applied for preservation and the significant impacts of cooling to deep cryogenic temperatures. Current methods of liquid cool storage and deep subzero preservation by controlled slow cooling or vitrification are outlined. The methods for reanimation of functional cells are discussed, along with the equipment and protocols essential to achieve this, and new technologies coming online to optimize these requirements. These provide a consensus document from many authors and centers across the world and a basis to understand the current real-world applications for a range of cells and tissues of clinical importance discussed in the second associated chapter.

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Development and Application of Cryoprotectants

Winter is coming: the future of cryopreservation

Article Open access 24 March 2021

Cryopreservation: Evolution of Molecular Based Strategies

Notes

1.
The use of the term mesenchymal stem cell (MSC) has been hotly debated in the literature as it actually applies to several different cell types. The term “tissue specific progenitor cells” has been proposed as a more accurate term (Robey 2017) and where the abbreviation “MSC” appears elsewhere in this chapter, it can be assumed this is a reference to mesenchymal stromal cells or the more generic term for this group of cell types tissue-specific progenitor cells (TSPCs).

Abbreviations

ATP:: Adenosine triphosphate
CPA:: Cryoprotective agent
Cryo-SEM:: Cryogenic-scanning electron microscopy
CSP:: Cold-shock protein
DMSO:: Dimethylsulfoxide
DNA:: Deoxyribonucleic acid
EG:: Ethylene glycol
ELISA:: Enzyme-linked immunosorbent assay
FBS:: Fetal bovine serum
FTIR:: Fourier-transform infrared spectroscopy
GMP:: Good manufacturing practice
HES:: Hydroxyethyl starch
HSC:: Hematopoietic stem cells
HSP:: Heat-shock protein
HTK:: Histidine-tryptophan-ketoglutarate
HTS:: Hypothermosol
IIF:: Intracellular ice formation
IRI:: Ice recrystallization inhibitor
LDH:: Lactate dehydrogenase
LN₂:: Liquid nitrogen
LT:: Liquidus tracking
MAP:: Mitogen-activated protein
mRNA:: Messenger ribonucleic acid
MSCs:: Mesenchymal stromal cells
NMR:: Nuclear magnetic resonance spectroscopy
O-PTIR:: Optical photothermal infrared spectroscopy
PCR:: Polymer chain reaction
PEO:: Polyethylene oxide
PI:: Propidium iodide
PTFE:: Polytetrafluoroethylene
PVP:: Polyvinylpyrrolidone
ROS:: Reactive oxygen species
RT-PCR:: Real-time polymerase chain reaction
TECs:: Tissue-engineered constructs
TSPCs:: Tissue-specific progenitor cells
TUNEL:: Terminal deoxyribonucleotidyl transferase-mediated dUTP nick end-labeling
TWEs:: Transient warming events
UW:: University of Wisconsin
VS:: Vitrification solutions

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Web Links

ASKION. https://www.askion.com/
Brooks Life Sciences. https://www.brookslifesciences.com/
Cytiva. https://www.cytivalifesciences.com/en/us/shop/cell-therapy/systems/via-freeze-controlled-rate-freezers-p-09721#overview
Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine. http://www.cryo.org.ua
PLANER. https://planer.com/products/storage-vessels/cryomacs/accessories-a-consumables.html
Society for Low Temperature Biology Committee. https://www.sltb.info
United States Food and Drug Administration (U.S. FDA). http://www.fda.gov

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Acknowledgments

Development of this chapter is facilitated by the UNESCO Chair in Cryobiology hosted by the Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine (http://www.cryo.org.ua) and supported by the Society for Low Temperature Biology Committee (https://www.sltb.info).

Figure 1 was created by William E. Reid.

Funded by Chinese Academy of Sciences President’s International Fellowship for Special Experts. Grant No. 2018FSB0009

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Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine
Iryna A. Buriak, Anatoliy M. Goltsev, Alexander Yu. Petrenko & Natalia A. Trufanova
Department of Mechanical Engineering and Engineering Science, University of North Carolina at Charlotte, Charlotte, NC, USA
Gloria Elliott
Centre for Ultrastructural Imaging, King’s College London, London, UK
Roland A. Fleck
Division of Surgery, UCL Medical School, Royal Free Hospital Campus, London, UK
Barry J. Fuller
Institute for Multiphase Processes & Center for Biomedical Engineering Leibniz Universität Hannover, Hannover, Germany
Birgit Glasmacher, Oleksandr Gryshkov, Janina Hagedorn, Vitalii Mutsenko, Tim Rittinghaus & Sara Leal-Marin
Centro Binacional (Argentina-Italia) de Investigaciones en Criobiología Clínica y Aplicada, Universidad Nacional de Rosario and Consejo Nacional de Investigaciones Científicas y Técnicas, Rosario, Argentina
Edgardo E. Guibert, Leonardo Juan de Paz & María Celeste Robert
V.N. Karazin Kharkiv National University, Kharkiv, Ukraine
Alexander Yu. Petrenko
Institute of Experimental Medicine of the CAS, Prague, Czech Republic
Yuriy Petrenko
International Stem Cell Banking Initiative, Barley, UK
Glyn Nigel Stacey
National Stem Cell Resource Centre, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
Glyn Nigel Stacey
Innovation Academy for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China
Glyn Nigel Stacey

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Iryna A. Buriak
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Correspondence to Barry J. Fuller or Glyn Nigel Stacey .

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Tulane Univ. Sch. of Medicine, New Orleans, LA, USA
Jeffrey M. Gimble
AUVA Center, Ludwig Boltzmann Institute for Experimen, Wien, Wien, Austria
Darja Marolt Presen
Human Development and Health, University of Southampton, Southampton, UK
Richard O. C. Oreffo
AUVA Center, Ludwig Boltzmann Institute for Experimen, Vienna, Austria
Susanne Wolbank
Experimental and Clinical Traumatol, Ludwig Boltzmann Institute for, Wien, Austria
Heinz Redl

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Buriak, I.A. et al. (2022). Preservation and Storage of Cells for Therapy: Fundamental Aspects of Low Temperature Science. In: Gimble, J.M., Marolt Presen, D., Oreffo, R.O.C., Wolbank, S., Redl, H. (eds) Cell Engineering and Regeneration. Reference Series in Biomedical Engineering(). Springer, Cham. https://doi.org/10.1007/978-3-319-37076-7_67-1

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DOI: https://doi.org/10.1007/978-3-319-37076-7_67-1
Received: 06 August 2021
Accepted: 01 December 2021
Published: 16 February 2022
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-37076-7
Online ISBN: 978-3-319-37076-7
eBook Packages: Springer Reference EngineeringReference Module Computer Science and Engineering

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Preservation and Storage of Cells for Therapy: Fundamental Aspects of Low Temperature Science

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DOI: https://doi.org/10.1007/978-3-319-37076-7_67-2
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Preservation and Storage of Cells for Therapy: Fundamental Aspects of Low Temperature Science

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DOI: https://doi.org/10.1007/978-3-319-37076-7_67-1

Preservation and Storage of Cells for Therapy: Fundamental Aspects of Low Temperature Science

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Development and Application of Cryoprotectants

Winter is coming: the future of cryopreservation

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Preservation and Storage of Cells for Therapy: Fundamental Aspects of Low Temperature Science

Abstract

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Development and Application of Cryoprotectants

Winter is coming: the future of cryopreservation

Cryopreservation: Evolution of Molecular Based Strategies

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Abbreviations

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Acknowledgments

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