Abstract
Electroporation (also termed as pulsed electric field–PEF) has been used in several fields, such as clinics (electrochemotherapy, gene electrotransfer, irreversible tissue ablation, DNA vaccination); food processing (inactivation of microorganisms, drying, extraction of juice from fruits and vegetables); and biotechnology (bacterial electrotransformation, extraction of technologically relevant molecules from microorganisms). Later use of PEF has only started to emerge and the yields of extracted molecules are described to be the same or higher as obtained by other methods. Furthermore, several distinctive advantages over the standard techniques have been described, such as less fragmentation of microorganism and by that less purification needed; no need to use expensive and harmful chemicals and possible selective extraction of molecules. In the introduction, benefits and shortfalls of chemical and physical extraction methods are described. Subsequent sections explore in more detail assisted extraction of different molecules (proteins, plasmid DNA, lipids) by PEF from bacterial and yeast cells. Firstly, instantaneous plasmid DNA (pDNA) extraction/transfer from/into various species is described, where different parameters have been studied in order to obtain as much successfully transformed cells as possible. Later sections present an overview of the parameters affecting the efficiency of extraction assisted by PEF. At the end main parameters affecting the yield of extracted molecules are summarized and main challenges of PEF-assisted extraction of molecules from microorganisms are presented.
Similar content being viewed by others
References
Baulard A, Jourdan C, Mercenier A, Locht C (1992) Rapid mycobacterial plasmid analysis by electroduction between Mycobacterium spp. and Escherichia coli. Nucleic Acids Res 20:4105. doi:10.1093/nar/20.15.4105
Calvin NM, Hanawalt PC (1988) High-efficiency transformation of bacterial cells by electroporation. J Bacteriol 170:2796–2801
Coustets M, Ganeva V, Galutzov B, Teissie J (2015) Millisecond duration pulses for flow-through electro-induced protein extraction from E-coli and associated eradication. Bioelectrochemistry 103:82–91. doi:10.1016/j.bioelechem.2014.08.008
Ganeva V, Galutzov B, Teissie J (2013) Evidence that pulsed electric field treatment enhances the cell wall porosity of yeast cells. Appl Biochem Biotechnol 172:1540–1552. doi:10.1007/s12010-013-0628-x
Gao Y, Feng X, Xian M et al (2013) Inducible cell lysis systems in microbial production of bio-based chemicals. Appl Microbiol Biotechnol 97:7121–7129. doi:10.1007/s00253-013-5100-x
Gunn L, Nickoloff JA (1995) Rapid transfer of low copy-number episomal plasmids from Saccharomyces cerevisiae to Escherichia coli by electroporation. Mol Biotechnol 3:79–84. doi:10.1007/BF02789103
Haberl Meglic S, Marolt T, Miklavcic D (2015) Protein extraction by means of electroporation from E. coli with preserved viability. J Membr Biol. doi:10.1007/s00232-015-9824-7
Haberl S, Jarc M, Strancar A et al (2013) Comparison of alkaline lysis with electroextraction and optimization of electric pulses to extract plasmid DNA from Escherichia coli. J Membr Biol 246:861–867. doi:10.1007/s00232-013-9580-5
Haberl-Meglič S, Levičnik E, Luengo E et al (2016) The effect of temperature and bacterial growth phase on protein extraction by means of electroporation. Bioelectrochemistry 112:77–82. doi:10.1016/j.bioelechem.2016.08.002
Kilbane JJ, Bielaga BA (1991) Instantaneous gene transfer from donor to recipient microorganisms via electroporation. Biotechniques 10:354–365
Kotnik T (2013) Prokaryotic diversity, electrified DNA, lightning waveforms, abiotic gene transfer, and the Drake equation: assessing the hypothesis of lightning-driven evolution. Phys Life Rev 10:384–388. doi:10.1016/j.plrev.2013.07.027
Kotnik T, Frey W, Sack M et al (2015) Electroporation-based applications in biotechnology. Trends Biotechnol 33:480–488. doi:10.1016/j.tibtech.2015.06.002
Marcil R, Higgins DR (1992) Direct transfer of plasmid DNA from yeast to E. coli by electroporation. Nucleic Acids Res 20:917
Matos T, Senkbeil S, Mendonca A et al (2013) Nucleic acid and protein extraction from electropermeabilized E. coli cells on a microfluidic chip. Analyst 138:7347–7353. doi:10.1039/c3an01576a
Moser D, Zarka D, Hedman C, Kallas T (1995) Plasmid and chromosomal DNA recovery by electroextraction of cyanobacteria. FEMS Microbiol Lett 128:307–313. doi:10.1111/j.1574-6968.1995.tb07541.x
Naglak TJ, Hettwer DJ, Wang HY (1990) Chemical permeabilization of cells for intracellular product release. Bioprocess Technol 9:177–205
Ohshima T, Sato M, Saito M (1995) Selective release of intracellular protein using pulsed electric field. J Electrostat 35:103–112. doi:10.1016/0304-3886(95)00014-2
Ohshima T, Hama Y, Sato M (2000) Releasing profiles of gene products from recombinant Escherichia coli in a high-voltage pulsed electric field. Biochem Eng J 5:149–155. doi:10.1016/S1369-703X(00)00055-3
Pfau J, Youderian P (1990) Transferring plasmid DNA between different bacterial species with electroporation. Nucleic Acids Res 18:6165. doi:10.1093/nar/18.20.6165
Saccardo P, Corchero JL, Ferrer-Miralles N (2016) Tools to cope with difficult-to-express proteins. Appl Microbiol Biotechnol 100:4347–4355. doi:10.1007/s00253-016-7514-8
Sheng J, Vannela R, Rittmann BE (2011) Evaluation of cell-disruption effects of pulsed-electric-field treatment of Synechocystis PCC 6803. Environ Sci Technol 45:3795–3802. doi:10.1021/es103339x
Shiina S, Ohshima T, Sato M (2007) Extracellular production of alpha-amylase during fed-batch cultivation of recombinant Escherichia coli using pulsed electric field. J Electrostat 65:30–36. doi:10.1016/j.elstat.2005.03.093
Suga M, Hatakeyama T (2009) Gene transfer and protein release of fission yeast by application of a high voltage electric pulse. Anal Bioanal Chem 394:13–16. doi:10.1007/s00216-009-2678-z
Summers D, Withers H (1990) Electrotransfer – direct transfer of bacterial plasmid DNA by electroporation. Nucleic Acids Res 18:2192. doi:10.1093/nar/18.8.2192
Takayuki O, Takahiro O, Masayuki S (1999) Decomposition of nucleic acid molecules in pulsed electric field and its release from recombinant Escherichia coli. J Electrostat 46:163–170. doi:10.1016/S0304-3886(99)00016-9
Ward LJH, Jarvis AW (1991) Rapid electroporation-mediated plasmid transfer between Lactococcus lactis and Escherichia coli without the need for plasmid preparation. Lett Appl Microbiol 13:278–280. doi:10.1111/j.1472-765X.1991.tb00628.x
Yarmush ML, Golberg A, Sersa G et al (2014) Electroporation-based technologies for medicine: principles, applications, and challenges. In: Yarmush ML (ed) Annual review of biomedical engineering, vol 16. Annual Reviews, Palo Alto, pp 295–320
Acknowledgment
This work was supported by the Slovenian Research Agency (Grant J7-6783), and conducted in the scope of the European Laboratory of Pulsed Electric Fields Applications (LEA EBAM) and within networking efforts of the COST Action TD1104 – European Network for Development of Electroporation-Based Technologies and Treatments (www.electroporation.net).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Section Editor information
Rights and permissions
Copyright information
© 2016 Springer International Publishing AG
About this entry
Cite this entry
Meglič, S.H. (2016). Pulsed Electric Fields-Assisted Extraction of Molecules from Bacterial and Yeast Cells. In: Miklavcic, D. (eds) Handbook of Electroporation. Springer, Cham. https://doi.org/10.1007/978-3-319-26779-1_131-1
Download citation
DOI: https://doi.org/10.1007/978-3-319-26779-1_131-1
Received:
Accepted:
Published:
Publisher Name: Springer, Cham
Online ISBN: 978-3-319-26779-1
eBook Packages: Springer Reference Biomedicine and Life SciencesReference Module Biomedical and Life Sciences
Publish with us
Chapter history
-
Latest
Pulsed Electric Fields-Assisted Extraction of Molecules from Bacterial and Yeast Cells- Published:
- 05 December 2016
DOI: https://doi.org/10.1007/978-3-319-26779-1_131-2
-
Original
Pulsed Electric Fields-Assisted Extraction of Molecules from Bacterial and Yeast Cells- Published:
- 26 October 2016
DOI: https://doi.org/10.1007/978-3-319-26779-1_131-1