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Pulsed Electric Fields-Assisted Extraction of Molecules from Bacterial and Yeast Cells

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Handbook of Electroporation

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Abstract

Electroporation (also termed as pulsed electric field–PEF) has been used in several fields, such as clinics (electrochemotherapy, gene electrotransfer, irreversible tissue ablation, DNA vaccination); food processing (inactivation of microorganisms, drying, extraction of juice from fruits and vegetables); and biotechnology (bacterial electrotransformation, extraction of technologically relevant molecules from microorganisms). Later use of PEF has only started to emerge and the yields of extracted molecules are described to be the same or higher as obtained by other methods. Furthermore, several distinctive advantages over the standard techniques have been described, such as less fragmentation of microorganism and by that less purification needed; no need to use expensive and harmful chemicals and possible selective extraction of molecules. In the introduction, benefits and shortfalls of chemical and physical extraction methods are described. Subsequent sections explore in more detail assisted extraction of different molecules (proteins, plasmid DNA, lipids) by PEF from bacterial and yeast cells. Firstly, instantaneous plasmid DNA (pDNA) extraction/transfer from/into various species is described, where different parameters have been studied in order to obtain as much successfully transformed cells as possible. Later sections present an overview of the parameters affecting the efficiency of extraction assisted by PEF. At the end main parameters affecting the yield of extracted molecules are summarized and main challenges of PEF-assisted extraction of molecules from microorganisms are presented.

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Acknowledgment

This work was supported by the Slovenian Research Agency (Grant J7-6783), and conducted in the scope of the European Laboratory of Pulsed Electric Fields Applications (LEA EBAM) and within networking efforts of the COST Action TD1104 – European Network for Development of Electroporation-Based Technologies and Treatments (www.electroporation.net).

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Authors and Affiliations

  1. Faculty of Electrical Engineering, University of Ljubljana, Trzaska 25, 1000, Ljubljana, Slovenia

    Saša Haberl Meglič

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  1. Saša Haberl Meglič
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Correspondence to Saša Haberl Meglič .

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Editors and Affiliations

  1. Faculty of Electrical Enginer, University of Ljubljana, Ljubljana, Slovenia

    Damijan Miklavcic

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  1. Department of Animal Production and Food Science, Food Technology, Faculty of Veterinary, University of Zaragoza, C/Miguel Servet, 177, 50013, Zaragoza, Spain

    Javier Raso

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Haberl Meglič, S. (2016). Pulsed Electric Fields-Assisted Extraction of Molecules from Bacterial and Yeast Cells. In: Miklavcic, D. (eds) Handbook of Electroporation. Springer, Cham. https://doi.org/10.1007/978-3-319-26779-1_131-2

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  • DOI: https://doi.org/10.1007/978-3-319-26779-1_131-2

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  • Publisher Name: Springer, Cham

  • Online ISBN: 978-3-319-26779-1

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  1. Latest

    Pulsed Electric Fields-Assisted Extraction of Molecules from Bacterial and Yeast Cells
    Published:
    05 December 2016

    DOI: https://doi.org/10.1007/978-3-319-26779-1_131-2

  2. Original

    Pulsed Electric Fields-Assisted Extraction of Molecules from Bacterial and Yeast Cells
    Published:
    26 October 2016

    DOI: https://doi.org/10.1007/978-3-319-26779-1_131-1

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