Abstract
Maize Activator (Ac) is one of the prototype transposable elements of the hAT transposon superfamily, members of which were identified in plants, fungi, and animals. The autonomous Ac and nonautonomous Dissociation (Ds) elements are mobilized by the single transposase protein encoded by Ac. To date Ac/Ds transposons were shown to be functional in approximately 20 plant species and have become the most widely used transposable elements for gene tagging and functional genomics approaches in plants. In this chapter we review the biology, regulation, and transposition mechanism of Ac/Ds elements in maize and heterologous plants. We discuss the parameters that are known to influence the functionality and transposition efficiency of Ac/Ds transposons and need to be considered when designing Ac transposase expression constructs and Ds elements for application in heterologous plant species.
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References
McClintock B (1951) Chromosome organization and genic expression. Cold Spring Harb Symp Quant Biol 16:13–47
Oliver KR, Greene WK (2009) Transposable elements: powerful facilitators of evolution. Bioessays 31:703–714
Zeh DW, Zeh JA, Ishida Y (2009) Transposable elements and an epigenetic basis for punctuated equilibria. Bioessays 31:715–726
Arensburger P et al (2011) Phylogenetic and functional characterization of the hAT transposon superfamily. Genetics 188:45–57
Kempken F, Windhofer F (2001) The hAT family: a versatile transposon group common to plants, fungi, animals, and man. Chromosoma 110:1–9
Kunze R, Weil CF (2002) The hAT and CACTA superfamilies of plant transposons. In: Craig NL, Craigie R, Gellert M et al (eds) Mobile DNA II. ASM Press, Washington, pp 565–610
Essers L, Adolphs RH, Kunze R (2000) A highly conserved domain of the maize Activator transposase is involved in dimerization. Plant Cell 12:211–224
Kunze R, Starlinger P (1989) The putative transposase of transposable element Ac from Zea mays L. interacts with subterminal sequences of Ac. EMBO J 8:3177–3185
Coupland G et al (1989) Sequences near the termini are required for transposition of the maize transposon Ac in transgenic tobacco plants. Proc Natl Acad Sci USA 86:9385–9388
Kunze R et al (1987) Transcription of transposable element Activator (Ac) of Zea mays L. EMBO J 6:1555–1563
Coupland G et al (1988) Characterization of the maize transposable element Ac by internal deletions. EMBO J 7:3653–3659
Du C et al (2011) The complete Ac/Ds transposon family of maize. BMC Genomics 12:588
Xiao YL, Peterson T (2002) Ac transposition is impaired by a small terminal deletion. Mol Genet Genomics 266:720–731
Chatterjee S, Starlinger P (1995) The role of subterminal sites of transposable element Ds of Zea mays in excision. Mol Gen Genet 249:281–288
Döring H-P, Starlinger P (1986) Molecular genetics of transposable elements in plants. Annu Rev Genet 20:175–200
Martinez-Ferez IM, Dooner HK (1997) Sesqui-Ds, the chromosome-breaking insertion at bz-m1, links double Ds to the original Ds element. Mol Gen Genet 255:580–586
McClintock B (1948) Mutable loci in maize. Carnegie Inst Wash Yr Bk 47:155–169
Döring H-P et al (1989) Double Ds elements are involved in specific chromosome breakage. Mol Gen Genet 219:299–305
Dooner HK, Belachew A (1991) Chromosome breakage by pairs of closely linked transposable elements of the Ac-Ds family in maize. Genetics 129:855–862
Weil CF, Wessler SR (1993) Molecular evidence that chromosome breakage by Ds elements is caused by aberrant transposition. Plant Cell 5:515–522
Zhang J, Peterson T (1999) Genome rearrangements by nonlinear transposons in maize. Genetics 153:1403–1410
Zhang J, Peterson T (2004) Transposition of reversed Ac element ends generates chromosome rearrangements in maize. Genetics 167:1929–1937
Zhang J, Peterson T (2005) A segmental deletion series generated by sister-chromatid transposition of Ac transposable elements in maize. Genetics 171:333–344
Zhang J, Zhang F, Peterson T (2006) Transposition of reversed Ac element ends generates novel chimeric genes in maize. PLoS Genet 2:e164
Zhang J et al (2009) Alternative Ac/Ds transposition induces major chromosomal rearrangements in maize. Genes Dev 23:755–765
Yu C et al (2010) Spatial configuration of transposable element Ac termini affects their ability to induce chromosomal breakage in maize. Plant Cell 22:744–754
Huang JT, Dooner HK (2008) Macrotransposition and other complex chromosomal restructuring in maize by closely linked transposons in direct orientation. Plant Cell 20:2019–2032
Ralston EJ, English J, Dooner HK (1989) Chromosome-breaking structure in maize involved in a fractured Ac element. Proc Natl Acad Sci USA 86:9451–9455
Yu C, Zhang J, Peterson T (2011) Genome rearrangements in maize induced by alternative transposition of reversed Ac/Ds termini. Genetics 188:59–67
Krishnaswamy L, Zhang J, Peterson T (2008) Reversed end Ds element: a novel tool for chromosome engineering in Arabidopsis. Plant Mol Biol 68:399–411
Xuan YH et al (2011) Transposon Ac/Ds-induced chromosomal rearrangements at the rice OsRLG5 locus. Nucleic Acids Res 39:e149
Belzile F, Yoder JI (1994) Unstable transmission and frequent rearrangement of two closely linked transposed Ac elements in transgenic tomato. Genome 37:832–839
English J, Harrison K, Jones JDG (1993) A genetic analysis of DNA sequence requirements for dissociation state I activity in tobacco. Plant Cell 5:501–514
English JJ, Harrison K, Jones JDG (1995) Aberrant transpositions of maize double Ds-like elements usually involve Ds ends on sister chromatids. Plant Cell 7:1235–1247
Yu C et al (2012) A transgenic system for generation of transposon Ac/Ds-induced chromosome rearrangements in rice. Theor Appl Genet 125:1449–1462
Wang L, Heinlein M, Kunze R (1996) Methylation pattern of activator (Ac) transposase binding sites in maize endosperm. Plant Cell 8:747–758
Wang L, Kunze R (1998) Transposase binding site methylation in the epigenetically inactivated Ac derivative Ds-cy. Plant J 13:577–582
Sutton WD et al (1984) Molecular analysis of Ds controlling element mutations at the Adh1 locus of maize. Science 223:1265–1268
Bravo-Angel AM et al (1995) The binding motifs for Ac transposase are absolutely required for excision of Ds1. Mol Gen Genet 248:527–534
Caldwell EEO, Peterson PA (1992) The Ac and Uq transposable element systems in maize: interactions among components. Genetics 131:723–731
Boehm U et al (1995) One of three nuclear localization signals of maize Activator (Ac) transposase overlaps the DNA-binding domain. Plant J 7:441–451
Feldmar S, Kunze R (1991) The ORFa protein, the putative transposase of maize transposable element Ac, has a basic DNA binding domain. EMBO J 10:4003–4010
Becker H-A, Kunze R (1997) Maize Activator transposase has a bipartite DNA binding domain that recognizes subterminal motifs and the terminal inverted repeats. Mol Gen Genet 254:219–230
Aravind L (2000) The BED finger, a novel DNA-binding domain in chromatin-boundary-element-binding proteins and transposases. Trends Biochem Sci 25:421–423
Nesmelova IV, Hackett PB (2010) DDE transposases: structural similarity and diversity. Adv Drug Deliv Rev 62:1187–1195
Kunze R et al (1993) Dominant transposition-deficient mutants of maize Activator (Ac) transposase. Proc Natl Acad Sci USA 90:7094–7098
Calvi BR et al (1991) Evidence for a common evolutionary origin of inverted repeat transposons in Drosophila and plants: hobo, Activator and Tam3. Cell 66:465–471
Hehl R et al (1991) Structural analysis of Tam3, a transposable element from Antirrhinum majus, reveals homologies to the Ac element from maize. Plant Mol Biol 16:369–371
Essers L, Kunze R (1995) Transposable elements Bg (Zea mays) and Tag1 (Arabidopsis thaliana) encode protein sequences with homology to Ac-like transposases. Maize Genet Coop Newsl 69:39–41
Kunze R, Saedler H, Lönnig W-E (1997) Plant transposable elements. In: Callow JA (ed) Adv Bot Res, vol 27. Academic, London, pp 331–470
Capy P et al (1997) Do the integrases of LTR-retrotransposons and class II element transposases have a common ancestor? Genetica 100:63–72
Haren L, Ton-Hoang B, Chandler M (1999) Integrating DNA: transposases and retroviral integrases. Annu Rev Microbiol 53:245–281
Zhou L et al (2004) Transposition of hAT elements links transposable elements and V(D)J recombination. Nature 432:995–1001
Yuan YW, Wessler SR (2011) The catalytic domain of all eukaryotic cut-and-paste transposase superfamilies. Proc Natl Acad Sci USA 108:7884–7889
Hickman AB et al (2005) Molecular architecture of a eukaryotic DNA transposase. Nat Struct Mol Biol 12:715–721
Lazarow K et al (2012) A hyperactive transposase of the maize transposable element activator (ac). Genetics 191:747–756
Li M-g, Starlinger P (1990) Mutational analysis of the N terminus of the protein of maize transposable element Ac. Proc Natl Acad Sci USA 87:6044–6048
Heinlein M, Brattig T, Kunze R (1994) In vivo aggregation of maize activator (Ac) transposase in nuclei of maize endosperm and petunia protoplasts. Plant J 5:705–714
Emelyanov A et al (2006) Trans-kingdom transposition of the maize dissociation element. Genetics 174:1095–1104
Kunze R et al (1995) Somatic and germinal activities of maize activator (Ac) transposase mutants in transgenic tobacco. Plant J 8:45–54
Lazarow K, Lütticke S (2009) An Ac/Ds-mediated gene trap system for functional genomics in barley. BMC Genomics 10:55
Weil CF, Kunze R (2000) Transposition of maize Ac/Ds transposable elements in the yeast Saccharomyces cerevisiae. Nat Genet 26:187–190
Yu J et al (2004) Microhomology-dependent end joining and repair of transposon-induced DNA hairpins by host factors in Saccharomyces cerevisiae. Mol Cell Biol 24:1351–1364
Boon Ng GH, Gong Z (2011) Maize Ac/Ds transposon system leads to highly efficient germline transmission of transgenes in medaka (Oryzias latipes). Biochimie 93:1858–1864
Froschauer A et al (2012) Effective generation of transgenic reporter and gene trap lines of the medaka (Oryzias latipes) using the Ac/Ds transposon system. Transgenic Res 21:149–162
Michel K, Atkinson PW (2003) Nuclear localization of the Hermes transposase depends on basic amino acid residues at the N-terminus of the protein. J Cell Biochem 89:778–790
Coen ES, Carpenter R, Martin C (1986) Transposable elements generate novel spatial patterns of gene expression in Antirrhinum majus. Cell 47:285–296
Roth DB et al (1992) V(D)J recombination: broken DNA molecules with covalently sealed (hairpin) coding ends in scid mouse thymocytes. Cell 70:983–991
McBlane JF et al (1995) Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps. Cell 83:387–395
Lu CP et al (2006) Amino acid residues in Rag1 crucial for DNA hairpin formation. Nat Struct Mol Biol 13:1010–1015
Ma Y et al (2002) Hairpin opening and overhang processing by an Artemis/DNA-dependent protein kinase complex in nonhomologous end joining and V(D)J recombination. Cell 108:781–794
Huefner ND et al (2011) Breadth by depth: expanding our understanding of the repair of transposon-induced DNA double strand breaks via deep-sequencing. DNA Repair (Amst) 10:1023–1033
Rinehart TA, Dean C, Weil CF (1997) Comparative analysis of non-random DNA repair following Ac transposon excision in maize and Arabidopsis. Plant J 12:1419–1427
Namgoong SY, Harshey RM (1998) The same two monomers within a MuA tetramer provide the DDE domains for the strand cleavage and strand transfer steps of transposition. EMBO J 17:3775–3785
Kennedy AK, Haniford DB, Mizuuchi K (2000) Single active site catalysis of the successive phosphoryl transfer steps by DNA transposases: insights from phosphorothioate stereoselectivity. Cell 101:295–305
Williams TL et al (1999) Organization and dynamics of the Mu transpososome: recombination by communication between two active sites. Genes Dev 13:2725–2737
Vollbrecht E et al (2010) Genome-wide distribution of transposed dissociation elements in maize. Plant Cell 22:1667–1685
Liao GC, Rehm EJ, Rubin GM (2000) Insertion site preferences of the P transposable element in Drosophila melanogaster. Proc Natl Acad Sci USA 97:3347–3351
Bennetzen JL et al (1994) Active maize genes are unmodified and flanked by diverse classes of modified, highly repetitive DNA. Genome 37:565–576
Chen J, Greenblatt IM, Dellaporta SL (1987) Transposition of Ac from the P locus of maize into unreplicated chromosomal sites. Genetics 117:109–116
Kolkman JM et al (2005) Distribution of activator (Ac) throughout the maize genome for use in regional mutagenesis. Genetics 169:981–995
Schnable PS et al (2009) The B73 maize genome: complexity, diversity, and dynamics. Science 326:1112–1115
Cowperthwaite M et al (2002) Use of the transposon Ac as a gene-searching engine in the maize genome. Plant Cell 14:713–726
Athma P, Grotewold E, Peterson T (1992) Insertional mutagenesis of the maize P gene by intragenic transposition of Ac. Genetics 131:199–209
Dooner HK, Belachew A (1989) Transposition pattern of the maize element Ac from the bz-m2(Ac) allele. Genetics 122:447–457
Greenblatt IM (1984) A chromosomal replication pattern deduced from pericarp phenotypes resulting from movements of the transposable element, Modulator, in maize. Genetics 108:471–485
Moreno MA et al (1992) Reconstitutional mutagenesis of the maize P gene by short-range Ac transpositions. Genetics 131:939–956
Van Schaik NW, Brink RA (1959) Transpositions of modulator, a component of the variegated pericarp allele in maize. Genetics 44:725–738
Lawson EJR et al (1994) Modification of the 5′ untranslated leader region of the maize Activator element leads to increased activity in Arabidopsis. Mol Gen Genet 245:608–615
Scortecci KC et al (1999) Negative effect of the 5′-untranslated leader sequence on Ac transposon promoter expression. Plant Mol Biol 40:935–944
Lisch D (2009) Epigenetic regulation of transposable elements in plants. Annu Rev Plant Biol 60:43–66
McClintock B (1984) The significance of responses of the genome to challenge. Science 226:792–801
Brettell RIS, Dennis ES (1991) Reactivation of a silent Ac following tissue culture is associated with heritable alterations in its methylation pattern. Mol Gen Genet 229:365–372
Brutnell TP, Dellaporta SL (1994) Somatic inactivation and reactivation of Ac associated with changes in cytosine methylation and transposase expression. Genetics 138:213–225
Kunze R, Starlinger P, Schwartz D (1988) DNA methylation of the maize transposable element Ac interferes with its transcription. Mol Gen Genet 214:325–327
Fusswinkel H et al (1991) Detection and abundance of mRNA and protein encoded by transposable element Activator (Ac) in maize. Mol Gen Genet 225:186–192
Kohli A et al (2004) Dedifferentiation-mediated changes in transposition behavior make the activator transposon an ideal tool for functional genomics in rice. Mol Breeding 13:177–191
Brutnell TP, May BP, Dellaporta SL (1997) The Ac-st2 element of maize exhibits a positive dosage effect and epigenetic regulation. Genetics 147:823–834
Slotkin RK, Freeling M, Lisch D (2003) Mu killer causes the heritable inactivation of the mutator family of transposable elements in Zea mays. Genetics 165:781–797
Slotkin RK, Freeling M, Lisch D (2005) Heritable transposon silencing initiated by a naturally occurring transposon inverted duplication. Nat Genet 37:641–644
Heinlein M (1996) Excision patterns of activator (Ac) and dissociation (Ds) elements in Zea mays L: implications for the regulation of transposition. Genetics 144:1851–1869
Scofield SR, English JJ, Jones JDG (1993) High level expression of the activator transposase gene inhibits the excision of dissociation in tobacco cotyledons. Cell 75:507–517
Takumi S et al (1999) Variations in the maize Ac transposase transcript level and the Ds excision frequency in transgenic wheat callus lines. Genome 42:1234–1241
Grabundzija I et al (2010) Comparative analysis of transposable element vector systems in human cells. Mol Ther 18:1200–1209
Lohe AR, Hartl DL (1996) Autoregulation of mariner transposase activity by overproduction and dominant-negative complementation. Mol Biol Evol 13:549–555
Chen J, Greenblatt IM, Dellaporta SL (1992) Molecular analysis of Ac transposition and DNA replication. Genetics 130:665–676
Ros F, Kunze R (2001) Regulation of activator/dissociation transposition by replication and DNA methylation. Genetics 157:1723–1733
Houba-Hérin N, Domin M, Pedron J (1994) Transposition of a Ds element from a plasmid into the plant genome in Nicotiana plumbaginifolia protoplast-derived cells. Plant J 6:55–66
Laufs J et al (1990) Wheat dwarf virus Ac/Ds vectors: expression and excision of transposable elements introduced into various cereals by a viral replicon. Proc Natl Acad Sci USA 87:7752–7756
McElroy D et al (1997) Development of a simple transient assay for Ac/Ds activity in cells of intact barley tissue. Plant J 11:157–165
Sugimoto K et al (1994) Transposition of the maize Ds element from a viral vector to the rice genome. Plant J 5:863–871
Wirtz U, Osborne B, Baker B (1997) Ds excision from extrachromosomal geminivirus vector DNA is coupled to vector DNA replication in maize. Plant J 11:125–135
Baker B et al (1986) Transposition of the maize controlling element “Activator” in tobacco. Proc Natl Acad Sci USA 83:4844–4848
Baker B et al (1987) Phenotypic assay for excision of the maize controlling element Ac in tobacco. EMBO J 6:1547–1554
Yoder JI (1990) Rapid proliferation of the maize transposable element Activator in transgenic tomato. Plant Cell 2:723–730
Roberts MR et al (1990) Excision of the maize transposable element Ac in flax callus. Plant Cell Rep 9:406–409
Schmidt R, Willmitzer L (1989) The maize autonomous element activator (Ac) shows a minimal germinal excision frequency of 0.2–0.5 % in transgenic Arabidopsis thaliana plants. Mol Gen Genet 220:17–24
Yang CH, Ellis JG, Michelmore RW (1993) Infrequent transposition of Ac in lettuce, Lactuca sativa. Plant Mol Biol 22:793–805
McKenzie N, Wen LY, Dale J (2002) Tissue-culture enhanced transposition of the maize transposable element dissociation in Brassica oleracea var. ‘Italica’. Theor Appl Genet 105:23–33
Dean C et al (1992) Behavior of the maize transposable element Ac in Arabidopsis thaliana. Plant J 2:69–81
Hehl R, Baker B (1989) Induced transposition of Ds by a stable Ac in crosses of transgenic tobacco plants. Mol Gen Genet 217:53–59
Bancroft I et al (1992) Development of an efficient two-element transposon tagging system in Arabidopsis thaliana. Mol Gen Genet 233:449–461
Swinburne J et al (1992) Elevated levels of Activator transposase mRNA are associated with high frequencies of Dissociation excision in Arabidopsis. Plant Cell 4:583–595
Scofield SR et al (1992) Promoter fusions to the Activator transposase gene cause distinct patterns of Dissociation excision in tobacco cotyledons. Plant Cell 4:573–582
Becker D et al (1992) Control of excision frequency of maize transposable element Ds in Petunia protoplasts. Proc Natl Acad Sci USA 89:5552–5556
Dowe MF Jr, Roman GW, Klein AS (1990) Excision and transposition of two Ds transposons from the bronze mutable 4 derivative 6856 allele of Zea mays L. Mol Gen Genet 221:475–485
Balcells L, Coupland G (1994) The presence of enhancers adjacent to the Ac promoter increases the abundance of transposase mRNA and alters the timing of Ds excision in Arabidopsis. Plant Mol Biol 24:789–798
Long D et al (1993) Analysis of the frequency of inheritance of transposed Ds elements in Arabidopsis after activation by a CaMV 35S promoter fusion to the Ac transposase gene. Mol Gen Genet 241:627–636
Finnegan EJ et al (1993) Behaviour of modified Ac elements in flax callus and regenerated plants. Plant Mol Biol 22:625–633
Finnegan EJ et al (1988) Transcription of the maize transposable element Ac in maize seedlings and in transgenic tobacco. Mol Gen Genet 212:505–509
Takumi S et al (1999) Trans-activation of a maize Ds transposable element in transgenic wheat plants expressing the Ac transposase gene. Theor Appl Genet 98:947–953
Grevelding C et al (1992) High rates of Ac/Ds germinal transposition in Arabidopsis suitable for gene isolation by insertional mutagenesis. Proc Natl Acad Sci USA 89:6085–6089
Jarvis P, Belzile F, Dean C (1997) Inefficient and incorrect processing of the Ac transposase transcript in iae1 and wild-type Arabidopsis thaliana. Plant J 11:921–931
Lisson R et al (2010) Alternative splicing of the maize Ac transposase transcript in transgenic sugar beet (Beta vulgaris L.). Plant Mol Biol 74:19–32
Martin DJ et al (1997) Alternative processing of the maize Ac transcript in Arabidopsis. Plant J 11:933–943
Houba-Hérin N et al (1990) Excision of a Ds-like maize transposable element (AcΔ) in a transient assay in Petunia is enhanced by a truncated coding region of the transposable element Ac. Mol Gen Genet 224:17–23
Shen WH, Ramos C, Hohn B (1998) Excision of Ds1 from the genome of maize streak virus in response to different transposase-encoding genes. Plant Mol Biol 36:387–392
Ito T et al (2005) A resource of 5,814 dissociation transposon-tagged and sequence-indexed lines of Arabidopsis transposed from start loci on chromosome 5. Plant Cell Physiol 46:1149–1153
Parinov S et al (1999) Analysis of flanking sequences from dissociation insertion lines: a database for reverse genetics in Arabidopsis. Plant Cell 11:2263–2270
Kuromori T et al (2004) A collection of 11 800 single-copy Ds transposon insertion lines in Arabidopsis. Plant J 37:897–905
Pan X, Li Y, Stein L (2005) Site preferences of insertional mutagenesis agents in Arabidopsis. Plant Physiol 137:168–175
Zhang BD et al (1999) Cloning of the DNA fragments flanking Ds insertion sites in tobacco genome. Acta Phytophysiol Sinica 25:7–14
Meissner R et al (2000) Technical advance: a high throughput system for transposon tagging and promoter trapping in tomato. Plant J 22:265–274
Carroll BJ et al (1995) Germinal transpositions of the maize element Dissociation from T-DNA loci in tomato. Genetics 139:407–420
Cooper LD et al (2004) Mapping Ds insertions in barley using a sequence-based approach. Mol Genet Genomics 272:181–193
Zhao T et al (2006) Mapped Ds/T-DNA launch pads for functional genomics in barley. Plant J 47:811–826
Singh J et al (2006) High-frequency Ds remobilization over multiple generations in barley facilitates gene tagging in large genome cereals. Plant Mol Biol 62:937–950
Enoki H et al (1999) Ac as a tool for the functional genomics of rice. Plant J 19:605–613
Greco R et al (2003) Transpositional behaviour of an Ac/Ds system for reverse genetics in rice. Theor Appl Genet 108:10–24
Kim CM et al (2004) Rapid, large-scale generation of Ds transposant lines and analysis of the Ds insertion sites in rice. Plant J 39:252–263
Kolesnik T et al (2004) Establishing an efficient Ac/Ds tagging system in rice: large-scale analysis of Ds flanking sequences. Plant J 37:301–314
van Enckevort LJ et al (2005) EU-OSTID: a collection of transposon insertional mutants for functional genomics in rice. Plant Mol Biol 59:99–110
Jones JDG et al (1990) Preferential transposition of the maize element Activator to linked chromosomal locations in tobacco. Plant Cell 2:701–707
Dooner HK et al (1991) Variable patterns of transposition of the maize element Activator in tobacco. Plant Cell 3:473–482
Stuurman J et al (1996) Single-site manipulation of tomato chromosomes in vitro and in vivo using Cre-lox site-specific recombination. Plant Mol Biol 32:901–913
Stuurman J, Nijkamp HJJ, van Haaren MJJ (1998) Molecular insertion-site selectivity of Ds in tomato. Plant J 14:215–223
Keller J, Lim E, Dooner HK (1993) Preferential transposition of Ac to linked sites in Arabidopsis. Theor Appl Genet 86:585–588
Bancroft I, Dean C (1993) Transposition pattern of the maize element Ds in Arabidopsis thaliana. Genetics 134:1221–1229
Machida C et al (1997) Characterization of the transposition pattern of the Ac element in Arabidopsis thaliana using endonuclease I-SceI. Proc Natl Acad Sci USA 94:8675–8680
Ito T et al (2002) A new resource of locally transposed dissociation elements for screening gene-knockout lines in silico on the Arabidopsis genome. Plant Physiol 129:1695–1699
Smith D et al (1996) Characterization and mapping of Ds-GUS-T-DNA lines for targeted insertional mutagenesis. Plant J 10:721–732
Koprek T et al (2000) An efficient method for dispersing Ds elements in the barley genome as a tool for determining gene function. Plant J 24:253–263
Upadhyaya NM et al (2006) Dissociation (Ds) constructs, mapped Ds launch pads and a transiently-expressed transposase system suitable for localized insertional mutagenesis in rice. Theor Appl Genet 112:1326–1341
Mckenzie N, Dale PJ (2004) Mapping of transposable element dissociation inserts in Brassica oleracea following plant regeneration from streptomycin selection of callus. Theor Appl Genet 109:333–341
Nishal B, Tantikanjana T, Sundaresan V (2005) An inducible targeted tagging system for localized saturation mutagenesis in Arabidopsis. Plant Physiol 137:3–12
Sundaresan V et al (1995) Patterns of gene action in plant development revealed by enhancer trap and gene trap transposable elements. Genes Dev 9:1797–1810
Marsch-Martinez N et al (2002) Activation tagging using the En-I maize transposon system in Arabidopsis. Plant Physiol 129:1544–1556
Schneider A et al (2005) A transposon-based activation-tagging population in Arabidopsis thaliana (TAMARA) and its application in the identification of dominant developmental and metabolic mutations. FEBS Lett 579:4622–4628
Greco R et al (2004) Transcription and somatic transposition of the maize En/Spm transposon system in rice. Mol Genet Genomics 270:514–523
Kumar CS, Wing RA, Sundaresan V (2005) Efficient insertional mutagenesis in rice using the maize En/Spm elements. Plant J 44:879–892
Ahern KR et al (2009) Regional mutagenesis using dissociation in maize. Methods 49:248–254
Wang F et al (2010) An Ac transposon system based on maize chromosome 4S for isolating long-distance-transposed Ac tags in the maize genome. Genetica 138:1261–1270
Kuromori T et al (2006) A trial of phenome analysis using 4000 Ds-insertional mutants in gene-coding regions of Arabidopsis. Plant J 47:640–651
Zhang S et al (2003) Resources for targeted insertional and deletional mutagenesis in Arabidopsis. Plant Mol Biol 53:133–150
Muskett PR et al (2003) A resource of mapped dissociation launch pads for targeted insertional mutagenesis in the Arabidopsis genome. Plant Physiol 132:506–516
Panjabi P, Burma PK, Pental D (2006) Use of the transposable element Ac/Ds in conjunction with Spm/dSpm for gene tagging allows extensive genome coverage with a limited number of starter lines: functional analysis of a four-element system in Arabidopsis thaliana. Mol Genet Genomics 276:533–543
Park SH et al (2007) Analysis of gene-trap Ds rice populations in Korea. Plant Mol Biol 65:373–384
Eamens AL et al (2004) A bidirectional gene trap construct suitable for T-DNA and Ds-mediated insertional mutagenesis in rice (Oryza sativa L.). Plant Biotechnol J 2:367–380
Qu S et al (2008) A versatile transposon-based activation tag vector system for functional genomics in cereals and other monocot plants. Plant Physiol 146:189–199
Qu S et al (2009) Construction and application of efficient Ac-Ds transposon tagging vectors in rice. J Integr Plant Biol 51:982–992
Luan WJ et al (2008) An efficient field screening procedure for identifying transposants for constructing an Ac/Ds-based insertional-mutant library of rice. Genome 51:41–49
Ayliffe MA et al (2007) A barley activation tagging system. Plant Mol Biol 64:329–347
Mathieu M et al (2009) Establishment of a soybean (Glycine max Merr. L) transposon-based mutagenesis repository. Planta 229:279–289
Acknowledgments
This work was supported by Deutsche Forschungsgemeinschaft (DFG) grant KU-715/9 and the Dahlem Centre of Plant Sciences (DCPS).
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Lazarow, K., Doll, ML., Kunze, R. (2013). Molecular Biology of Maize Ac/Ds Elements: An Overview. In: Peterson, T. (eds) Plant Transposable Elements. Methods in Molecular Biology, vol 1057. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-568-2_5
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