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Peptide-Modified Gemini Surfactants: Preparation and Characterization for Gene Delivery

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Pharmaceutical Nanotechnology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2000))

Abstract

Diquaternary ammonium-based gemini surfactants have been investigated widely as nonviral gene delivery systems. These unique cationic lipids have versatility in their chemical structure, show relatively low toxicity, are able to compact genetic material (pDNA, RNA) into nano-sized lipoplexes, and can be easily produced. In addition, the gemini surfactants show significant improvement in the transfection activity and biocompatibility compared to other cationic lipids used as nonviral gene delivery agents. The successful applications of gemini surfactant-based lipoplexes as topical gene delivery systems in animal models indicate their potential as noninvasive carriers for genetic immunization, theranostic agents, and in other gene therapy treatments. Detailed physicochemical characterization of gemini surfactant lipoplexes is a key factor in terms of formulation optimization and elucidation of the cellular uptake and stability of the lipoplexes system. In this chapter, we describe in detail different formulation methods to prepare gemini surfactant lipoplexes and comprehensive physicochemical characterization. In addition, we illustrate general protocols for in vitro evaluations.

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Author information

Author notes

  1. Mays Al-Dulaymi and Waleed Mohammed-Saeid contributed equally to this work.

Authors and Affiliations

  1. College of Pharmacy and Nutrition, University of Saskatchewan, Saskatoon, SK, Canada

    Mays Al-Dulaymi, Waleed Mohammed-Saeid, Anas El-Aneed & Ildiko Badea

  2. College of Pharmacy, Taibah University, Madina, Saudi Arabia

    Waleed Mohammed-Saeid

Authors
  1. Mays Al-Dulaymi
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  2. Waleed Mohammed-Saeid
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  3. Anas El-Aneed
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  4. Ildiko Badea
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Corresponding author

Correspondence to Ildiko Badea .

Editor information

Editors and Affiliations

  1. Department of Pharmaceutical Sciences, College of Pharmacy, Midwestern University, Glendale, AZ, USA

    Volkmar Weissig

  2. Nanomedicine Center of Excellence in Translational Cancer Research, Midwestern University, Glendale, AZ, USA

    Tamer Elbayoumi

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Al-Dulaymi, M., Mohammed-Saeid, W., El-Aneed, A., Badea, I. (2019). Peptide-Modified Gemini Surfactants: Preparation and Characterization for Gene Delivery. In: Weissig, V., Elbayoumi, T. (eds) Pharmaceutical Nanotechnology. Methods in Molecular Biology, vol 2000. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9516-5_14

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  • DOI: https://doi.org/10.1007/978-1-4939-9516-5_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9515-8

  • Online ISBN: 978-1-4939-9516-5

  • eBook Packages: Springer Protocols

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