Abstract
Banks of genetically characterized cancer stem cells (CSCs) isolated from individual patients and grown as spheroids offer an invaluable approach to identify genetic determinants of drug resistance versus sensitivity, and to study new stem cell-directed therapies. Here, we describe our standardized procedure for in vitro drug screening on colorectal CSCs, taking irinotecan as an example.
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Acknowledgments
This work was financially supported by the Italian Association for Cancer Research (AIRC) 5 per Mille 9979 grant to RDM.
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De Angelis, M.L., De Maria, R., Baiocchi, M. (2018). How to Assess Drug Resistance in Cancer Stem Cells. In: Papaccio, G., Desiderio, V. (eds) Cancer Stem Cells. Methods in Molecular Biology, vol 1692. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7401-6_10
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DOI: https://doi.org/10.1007/978-1-4939-7401-6_10
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