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Circular Chromosome Conformation Capture in Plants

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Plant Genomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1610))

Abstract

The study of nuclear architecture promises novel insights into genome function and regulation. Hereby, quantitative methods based on chromosome conformation capture (3C) revolutionized the field, as they allow accurate and unbiased characterization of 3D genome organization of genomic regions of interest. The choice of the appropriate 3C derivate is crucial to acquire results suited for a specific research question. Circular 3C (4C) is the method of choice to study the genome-wide 3D architecture of a specific genomic region of interest. Here, I present a robust 4C protocol, established in Arabidopsis thaliana, which can be employed by any experienced molecular biologist and is applicable in various other plant species.

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Authors and Affiliations

  1. Institute of Human Genetics, UMR9002 CNRS-UM, Montpellier, France

    Stefan Grob

Authors
  1. Stefan Grob
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Corresponding author

Correspondence to Stefan Grob .

Editor information

Editors and Affiliations

  1. Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna Biocenter (VBC), Vienna, Austria

    Wolfgang Busch

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© 2017 Springer Science+Business Media LLC

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Grob, S. (2017). Circular Chromosome Conformation Capture in Plants. In: Busch, W. (eds) Plant Genomics. Methods in Molecular Biology, vol 1610. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7003-2_6

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  • DOI: https://doi.org/10.1007/978-1-4939-7003-2_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7001-8

  • Online ISBN: 978-1-4939-7003-2

  • eBook Packages: Springer Protocols

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