Abstract
High-throughput chromosome conformation capture (Hi-C) enables the global quantification of chromatin interaction frequency in eukaryotic nuclei. This method is based on in situ Hi-C, in which chromatin is cross-linked with formaldehyde, then digested with restriction enzyme. Biotin-labeled nucleotide is incorporated before the spatially adjacent DNA ends are ligated, making it possible to enrich specifically the chimeric ligation products for deep sequencing. In this chapter, we describe a modified in situ Hi-C protocol for the global chromatin interaction analysis in plants.
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Acknowledgments
We gratefully acknowledge financial support from the National key R&D Program of China (2018YFC1004500), the National Natural Science Foundation of China (31571347 to C.H.), and Southern University of Science and Technology (No.G02226301 and Y01501821 to C.H.).
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Wang, W., Niu, L., Hou, C. (2022). Interrogating Global Chromatin Interaction Network by High-Throughput Chromosome Conformation Capture (Hi-C) in Plants. In: Lambing, C. (eds) Plant Gametogenesis. Methods in Molecular Biology, vol 2484. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2253-7_5
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DOI: https://doi.org/10.1007/978-1-0716-2253-7_5
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-2253-7
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