Abstract
Th2 responses such as peripheral and tissue eosinophilia are characteristic features in the host animals infected with Strongyloides venezuelensis and Trichinella spiralis. Th2 responses are characterized by a specific profile of cytokines and chemokines induced during the course of infection. In this chapter, we describe the methodology that is utilized in our laboratories to study the production of cytokine, chemokine, and antibodies related to the eosinophilia seen in mice infected with the parasites. Furthermore, protocols are described for the different methods used to study eosinophil functions in the blood and tissues of these experimental models of parasitic infections.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Dixon H, Blanchard C, deSchoolmeester ML, Yuill NC, Christie JW, Rothenberg ME, Else KJ (2006) The role of Th2 cytokines, chemokines and parasite products in eosinophil recruitment to the gastrointestinal mucosa during helminth infection. Eur J Immunol 36:1753–1763
Mir A, Benahmed D, Igual R, Borras R, O’Connor JE, Moreno MJ, Rull S (2006) Eosinophil-selective mediators in human strongyloidiasis. Parasite Immunol 28:397–400
Galioto AM, Hess JA, Nolan TJ, Schad GA, Lee JJ, Abraham D (2006) Role of eosinophils and neutrophils in innate and adaptive protective immunity to larval Strongyloides stercoralis in mice. Infect Immun 74:5730–5738
Korenaga M, Hitoshi Y, Yamaguchi N, Sato Y, Takatsu K, Tada I (1991) The role of interleukin-5 in protective immunity to Strongyloides venezuelensis infection in mice. Immunology 72:502–507
Watanabe K, Sasaki O, Hamano S, Kishihara K, Nomoto K, Tada I, Aoki Y (2003) Strongyloides ratti: the role of interleukin-5 in protection against tissue migrating larvae and intestinal adult worms. J Helminth 77:355–361
Bruschi F, Korenaga M, Watanabe N (2008) Eosinophils and Trichinella infection: toxic for the parasite and the host? Trends Parasitol 24:462–467
Bruschi F, Korenaga M (2012) Eosinophils and eosinophilia in parasitic infections. In: Walsh GM (ed) Eosinophils – structure. Biological properties and role in disease. Nova Biomedical, New York, p 175
Del Prete G, Chiumiento L, Amedei A, Piazza M, D'Elios MM, Codolo G, De Bernard M, Masetti M, Bruschi F (2008) Immunosuppression of TH2 responses in Trichinella spiralis infection by Helicobacter pylori neutrophil-activating protein. J Allergy Clin Immunol 122:908–913
Beckstead JH, Halverson PS, Ries CA, Bainton DF (1981) Enzyme histochemistry and immunohistochemistry on biopsy specimens of pathologic human bone marrow. Blood 57:1088–1098
Grouls V, Helpap B (1981) Selective staining of eosinophils and the immature precursors in tissue sections and autoradiographs with Congo Red. Stain Technol 56:323–325
Tomasi VH, Pérez MA, Itoiz ME (2008) Modification of Luna’s technique for staining eosinophils in the hamster check pouch. Biotech Histochem 83:147–151
Gentilini MV, Nuñez GG, Roux ME, Venturiello SM (2011) Trichinella spiralis infection rapidly induces lung inflammatory response: the lung as the site of helminthocytotoxic activity. Immunobiol 216:1054–1063
Friend DS, Gurish MF, Austen KF, Hunt J, Stevens RL (2000) Senescent jejunal mast cells and eosinophils in the mouse preferentially translocate to the spleen and draining lymph node, respectively, during the recovery phase of helminth infection. J Immunol 165:344–352
Chiumiento L, Del Prete G, Codolo G, De Bernard M, Amedei A, Della Bella C, Piazza M, D'Elios S, Caponi L, D'Elios MM, Bruschi F (2011) Stimulation of TH1 response by Helicobacter pylori neutrophil activating protein decreases the protective role of IgE and eosinophils in experimental trichinellosis. Int J Immunopathol Pharmacol 24:895–903
Gansmüller A, Anteunis A, Venturiello SM, Bruschi F, Binaghi RA (1987) Antibody-dependent in-vitro cytotoxicity of newborn Trichinella spiralis larvae: nature of the cells involved. Parasite Immunol 9:281–292
Gon S, Saito S, Takeda Y, Miyata H, Takatsu K, Sendo F (1997) Apoptosis and in vivo distribution and clearance of eosinophils in normal and Trichinella spiralis-infected rats. J Leukoc Biol 62:309–317
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media, New York
About this protocol
Cite this protocol
Korenaga, M., Bruschi, F. (2014). Qualitative and Quantitative Studies of Eosinophils in Parasitic Infections. In: Walsh, G. (eds) Eosinophils. Methods in Molecular Biology, vol 1178. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1016-8_18
Download citation
DOI: https://doi.org/10.1007/978-1-4939-1016-8_18
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1015-1
Online ISBN: 978-1-4939-1016-8
eBook Packages: Springer Protocols