Abstract
The method of cell monolayer rheology enables quantifying average rheological properties of cell in a single experimental run of few millions cells together in a single layer. Here we describe step-by-step procedure as to how to employ a modified commercial rotational rheometer to run rheological measurement and detect average viscoelastic properties of cells while maintaining the necessary precision level at the same time.
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References
Chhabra RP, Richardson JF (2008) Non-Newtonian flow and applied rheology, 2nd edn. Butterworth-Heinemann, Oxford, pp 1–55
Steffe JF (1996) Rheological methods in food process engineering. Freeman Press, East Lansing
Verdier C, Etienne J, Duperray A et al (2009) Review: rheological properties of biological materials. Comptes Rendus Physique 10:790–811
Kollmannsberger P, Fabry B (2011) Linear and nonlinear rheology of living cells. Annu Rev Mater Res 41:75–97
Weissman-Shomer P, Fry M (1975) Chick embryo fibroblasts senescence in vitro: pattern of cell division and life span as a function of cell density. Mech Ageing Dev 4:159–166
Cai P, Mizutani Y, Tsuchiya M et al (2013) Quantifying cell-to-cell variation in power-law rheology. Biophys J 105:1093–1102
Fernández P, Heymann L, Ott A et al (2007) Shear rheology of a cell monolayer. New J Phys 9:419–447
Dakhil H, Gilbert DF, Malhotra D et al (2016) Measuring average rheological quantities of cell monolayers in the linear viscoelastic regime. Rheol Acta 55:527–536
Davies GA, Stokes JR (2005) On the gap error in parallel plate rheometry that arises from the presence of air when zeroing the gap. J Rheol 49:919–922
Davies GA, Stokes JR (2008) Thin film and high shear rheology of multiphase complex fluids. J Non-Newtonian Fluid Mech 148:73–87
Pipe CJ, Majmudar TS, Mckinley GH (2008) High shear rate viscometry. Rheol Acta 47:621–642
Dakhil H, Wierschem A (2014) Measuring low viscosities and high shear rates with a rotational rheometer in a thin-gap parallel-disk configuration. Appl Rheol 24:63795–63801
Dakhil H, Basu SK, Steiner S, Gerlach Y, Soller A, Pan S, Germann N, Leidenberger M, Kappes B, Wierschem A (2021) Buffered λ-DNA solutions at high shear rates. J Rheol 65:159–169
Acknowledgments
HD gratefully acknowledges financial support by Deutscher Akademischer Austauschdienst (DAAD), Germany, and the Ministry of Higher Education & Scientific Research, Iraq.
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Basu, S.K., Dakhil, H., Wierschem, A. (2023). Average Rheological Quantities of Cells in Monolayers. In: Friedrich, O., Gilbert, D.F. (eds) Cell Viability Assays. Methods in Molecular Biology, vol 2644. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3052-5_8
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DOI: https://doi.org/10.1007/978-1-0716-3052-5_8
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