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Cell Viability Assays

Methods and Protocols

  • Book
  • © 2023
  • Latest edition

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Editors:
  1. Oliver Friedrich

    1. Institute of Medical Biotechnology (MBT), Department of Chemical and Biological Engineering (CBI), Friedrich-Alexander-University Erlangen-Nüremberg, Erlangen, Germany

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  2. Daniel F. Gilbert

    1. Institute of Medical Biotechnology (MBT), Department of Chemical and Biological Engineering (CBI), Friedrich-Alexander-University Erlangen-Nüremberg, Erlangen, Germany

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2644)

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About this book

This updated edition explores assessing cell viability as a measure for cell fitness under conditions of physiological and patho-physiological stress as well as challenging conditions to cellular and tissue homeostasis, and accounts for the ongoing 2D-to-3D development with topics and assays that target cell viability, mobility, and functionality of tissues and organs, natural or bioartificial, in 3D. The book’s contents span a wide range of viability and functionality assays, from impedance spectroscopy to chemiluminescence, fluorescence and label-free optical detection methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Cell Viability Assays: Methods and Protocols, Second Edition serves as a valuable resource to the growing community in bioinspired life sciences, biomedical sciences, and biotechnology by providing more standardized protocols to probe the “wellbeing” of cells in various environments.

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Table of contents (30 protocols)

  1. Front Matter

    Pages i-xvii
    Download chapter PDF

  2. Single Cell Assays in 2D Environments

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Assaying Mitochondrial Respiration as an Indicator of Cellular Metabolism and Fitness

      • Natalia Smolina, Aleksandr Khudiakov, Anna Kostareva
      Pages 3-14

    3. The MTT Assay: A Method for Error Minimization and Interpretation in Measuring Cytotoxicity and Estimating Cell Viability

      • Mahshid Ghasemi, Sisi Liang, Quang Minh Luu, Ivan Kempson
      Pages 15-33

    4. Assaying Proliferation Characteristics of Cells Cultured Under Static Versus Periodic Conditions

      • Daniel F. Gilbert, Oliver Friedrich, Joachim Wiest
      Pages 35-45

    5. Network Reconstruction as a Novel High-Level Marker of Functional Neuronal Viability

      • Jana Katharina Dahlmanns, Marc Dahlmanns
      Pages 47-63

    6. Assaying Mitochondrial Function by Multiparametric Flow Cytometry

      • Hannah C. Sheehan, Jonathan L. Tilly, Dori C. Woods
      Pages 65-80

    7. High-Efficiency Single-Cell Electrical Impedance Spectroscopy

      • Yongxiang Feng, Liang Huang, Peng Zhao, Fei Liang, Wenhui Wang
      Pages 81-97

    8. Cell Viability Multiplexing: Quantification of Cellular Viability by Barcode Flow Cytometry and Computational Analysis

      • Valentina Giudice, Victoria Fonseca, Carmine Selleri, Massimo Gadina
      Pages 99-121

    9. Average Rheological Quantities of Cells in Monolayers

      • Santanu Kumar Basu, Haider Dakhil, Andreas Wierschem
      Pages 123-132

    10. Assaying Spontaneous Network Activity and Cellular Viability Using Multi-Well Microelectrode Arrays

      • Seline S. Choo, Jackson Y. Keever, Jasmine Brown, Jenna D. Strickland, Timothy J. Shafer
      Pages 133-154

    11. Quantitative Live-Cell Ca2+ Imaging During Isotropic Cell Stretch

      • Ulrike Schöler, Anna-Lena Merten, Sebastian Schürmann, Oliver Friedrich
      Pages 155-176

    12. Assessment of Cell Viability in Electrically Excitable Muscle Cells Through Intact Twitch Stimulation

      • Julian Bauer, Stewart I. Head, Oliver Friedrich
      Pages 177-192

    13. Evaluating Cellular Viability by iTRAQ Proteomic Profiling

      • Anne Poljak, Mark Raftery, Patsie Polly
      Pages 193-209

    14. Quantifying pH in Malaria Using pHluorin and Flow Cytometry

      • Jeffrey Agyapong, Petra Rohrbach
      Pages 211-224

    15. Cell Membrane State, Permeability, and Elasticity Assessment for Single Cells and Cell Ensembles

      • Nicolas Färber, Simon V. Neidinger, Christoph Westerhausen
      Pages 225-236

    16. Neutral Red Uptake Assay to Assess Cytotoxicity In Vitro

      • Robim M. Rodrigues, Marth Stinckens, Gamze Ates, Tamara Vanhaecke
      Pages 237-245

    17. Digital Holographic Microscopy to Assess Cell Behavior

      • Brad Bazow, Van K. Lam, Thuc Phan, Byung Min Chung, George Nehmetallah, Christopher B. Raub
      Pages 247-266

    18. Second Harmonic Generation Morphometry of Muscle Cytoarchitecture in Living Cells

      • Dominik Schneidereit, Stefanie Nübler, Oliver Friedrich
      Pages 267-285

    19. Optimization of Cell Viability Assays for Drug Sensitivity Screens

      • Peter Larsson, Toshima Z. Parris
      Pages 287-302
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Editors and Affiliations

  • Institute of Medical Biotechnology (MBT), Department of Chemical and Biological Engineering (CBI), Friedrich-Alexander-University Erlangen-Nüremberg, Erlangen, Germany

    Oliver Friedrich, Daniel F. Gilbert

Bibliographic Information

  • Book Title: Cell Viability Assays

  • Book Subtitle: Methods and Protocols

  • Editors: Oliver Friedrich, Daniel F. Gilbert

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3052-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-3051-8Published: 05 May 2023

  • Softcover ISBN: 978-1-0716-3054-9Due: 18 May 2024

  • eBook ISBN: 978-1-0716-3052-5Published: 04 May 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XVII, 502

  • Number of Illustrations: 1 b/w illustrations, 110 illustrations in colour

  • Topics: Cell Biology, Biotechnology

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