Abstract
Atomic force microscopy allows the determination of both mechanical and adhesive properties of living single cells and generation of high-resolution surface images. Here, we describe a method to determine the Young’s modulus of a cell and adhesion between a coated cantilever and a cell, as well as an overview of the analysis of the data obtained. Additionally, we point out typical and important pitfalls during the measurement and analysis.
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Hohmann, T., Dehghani, F. (2021). Measuring Mechanical and Adhesive Properties of Single Cells Using an Atomic Force Microscope. In: Stein, U.S. (eds) Metastasis. Methods in Molecular Biology, vol 2294. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1350-4_6
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DOI: https://doi.org/10.1007/978-1-0716-1350-4_6
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-1350-4
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