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Anther Culture in Sweet Pepper (Capsicum annuum L.)

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Doubled Haploid Technology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2288))

Abstract

Peppers have a prominent role in traditional cuisine of many different countries all around the world. This is why pepper is one of the most important crops worldwide. Production of doubled haploid (DH) pepper plants has been assessed by different approaches, but at present, the most efficient and universal method is by far anther culture, based on the use of the Dumas de Vaulx et al. protocol published in 1981, and adapted to the particularities of each specific pepper background. In this chapter, we present a method to produce pepper DHs by anther culture, based on the Dumas de Vaulx et al. protocol, but including a number of modifications which, in our experience, allow for a more efficient production DH plants in different pepper genotypes.

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Acknowledgments

This work was supported by grants AGL2017-88135-R and PID2020-115763RB-I00 to JMSS from Spanish MICINN jointly funded by FEDER

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Authors and Affiliations

  1. Cell Biology Group—COMAV Institute, Universitat Politècnica de València, Valencia, Spain

    Mireia Albiñana Palacios & Jose M. Seguí-Simarro

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  1. Mireia Albiñana Palacios
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  2. Jose M. Seguí-Simarro
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Correspondence to Jose M. Seguí-Simarro .

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Editors and Affiliations

  1. Cell Biology Group - COMAV Institute, Universitat Politècnica de València, Valencia, Spain

    Jose M. Segui-Simarro

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Palacios, M.A., Seguí-Simarro, J.M. (2021). Anther Culture in Sweet Pepper (Capsicum annuum L.). In: Segui-Simarro, J.M. (eds) Doubled Haploid Technology. Methods in Molecular Biology, vol 2288. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1335-1_17

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  • DOI: https://doi.org/10.1007/978-1-0716-1335-1_17

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1334-4

  • Online ISBN: 978-1-0716-1335-1

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