Abstract
Various natural and synthetic biomaterials have been applied as skin substitutes for regenerating damaged skin. Here, we describe a straightforward method for fabrication of a tissue-engineered skin substitute by seeding human fetal fibroblasts on acellular human amniotic membrane (HAM). Fetal fibroblasts are achieved from the skin of normal and non-macerated fetus of 11–14 weeks old after spontaneous pregnancy termination. Acellular HAM is obtained by separation of the outer membrane of the chorion and removing its epithelial cells.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Pouyani T, Papp S, Schaffer L (2012) Tissue-engineered fetal dermal matrices. In Vitro Cell Dev Biol Anim 48:493–506
Motamed S, Taghiabadi E, Molaei H, Sodeifi N, Hassanpour SE, Shafieyan S et al (2017) Cell-based skin substitutes accelerate regeneration of extensive burn wounds in rats. Am J Surg 214:762–769
Litwiniuk M, Grzela T (2014) Amniotic membrane: new concepts for an old dressing. Wound Repair Regen 22:451–456
Zuliani T, Saiagh S, Knol AC, Esbelin J, Dreno B (2013) Fetal fibroblasts and keratinocytes with immunosuppressive properties for allogeneic cell-based wound therapy. PLoS One 8:e70408
Niknejad H, Peirovi H, Jorjani M, Ahmadiani A, Ghanavi J, Seifalian AM (2008) Properties of the amniotic membrane for potential use in tissue engineering. Eur Cell Mater 15:88–99
Taghiabadi E, Nasri S, Shafieyan S, Jalili Firoozinezhad S, Aghdami N (2015) Fabrication and characterization of spongy denuded amniotic membrane based scaffold for tissue engineering. Cell J 16:476–487
Hao Y, Ma DH, Hwang DG, Kim WS, Zhang F (2000) Identification of antiangiogenic and antiinflammatory proteins in human amniotic membrane. Cornea 19:348–352
Tseng SC, Li DQ, Ma X (1999) Suppression of transforming growth factor-beta isoforms, TGF-beta receptor type II, and myofibroblast differentiation in cultured human corneal and limbal fibroblasts by amniotic membrane matrix. J Cell Physiol 179:325–335
Stock SJ, Kelly RW, Riley SC, Calder AA (2007) Natural antimicrobial production by the amnion. Am J Obstet Gynecol 196(255):e1–e6
Acknowledgments
The protocols described here were developed under the support of a Royan institute grant.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media New York
About this protocol
Cite this protocol
Taghiabadi, E., Beiki, B., Aghdami, N., Bajouri, A. (2018). Amniotic Membrane Seeded Fetal Fibroblasts as Skin Substitute for Wound Regeneration. In: Turksen, K. (eds) Skin Stem Cells. Methods in Molecular Biology, vol 1879. Humana Press, New York, NY. https://doi.org/10.1007/7651_2018_135
Download citation
DOI: https://doi.org/10.1007/7651_2018_135
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8869-3
Online ISBN: 978-1-4939-8870-9
eBook Packages: Springer Protocols