Abstract
The rich phytochemical profile of Hypericum species and the presence of unique compounds, such as hypericins and hyperforin, make them medicinally valuable worldwide. Among Hypericum spp., H. perforatum L. remains the most investigated and exploited species for specific compound production, while others have been surveyed mainly as a source of novel drugs. The main focus of Hypericum biotechnology is the manipulation of secondary metabolism and development of environmentally sustainable and economically viable culture systems toward the efficient production of target and novel secondary metabolites. Since specific compounds accumulate mainly in the aerial parts of Hypericum plants, large-scale shoot cultures are viewed as superior alternative for the production of these compounds under controlled conditions to their extraction from wild- or greenhouse-grown plants or their chemical synthesis. This chapter describes how media culture composition, culture conditions, elicitors, and other critical parameters influence the behavior of Hypericum spp. in shoot cultures and how optimization of these factors could allow improvements in secondary metabolite-related production and discovery of novel drugs.
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Abbreviations
- MS:
-
Murashige and Skoog
- LS:
-
Linsmaier and Skoog
- B5:
-
Gamborg B-5
- PGRs:
-
Plant growth regulators
- IBA :
-
Indole-3-butyric acid
- IAA:
-
Indole-3-acetic acid
- BAP:
-
6-Benzylaminopurine
- Kin:
-
Kinetin
- NAA:
-
1-Naphthaleneacetic acid
- TDZ :
-
Thidiazuron
- JA:
-
Jasmonic acid
- SA:
-
Salicylic acid
- MJA:
-
Methyl jasmonate
- DHPJA:
-
2,3-Dihydroxypropyl jasmonate
- Z:
-
Zeatin
- PEG:
-
Polyethylene glycol
- PEC:
-
Pectin
- CHI:
-
Chitin
- DEX:
-
Dextran
- FCM:
-
Flow cytometry
- DW:
-
Dry weight
- FW:
-
Fresh weight
- PI:
-
Partial immersion
- PB:
-
Paper bridge
- spp.:
-
Species
- H.:
-
Hypericum
- A. :
-
Agrobacterium
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Acknowledgments
This work was partially supported by a grant of the Ministry of Research and Innovation through Program 1 – Development of the National R&D System Subprogram 1.2 – Institutional Performance – Projects for Excellence Financing in RDI ctr. no. 22PFE/2018 and partially by the core program PN2019-2022 – BIODIVERS 3 through the project BIOSERV project code 19270201 contract nr. 25 N/2019.
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Coste, A., Pop, C., Halmagyi, A., Butiuc-Keul, A. (2021). Secondary Metabolites in Shoot Cultures of Hypericum. In: Ramawat, K.G., Ekiert, H.M., Goyal, S. (eds) Plant Cell and Tissue Differentiation and Secondary Metabolites. Reference Series in Phytochemistry. Springer, Cham. https://doi.org/10.1007/978-3-030-30185-9_9
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