Abstract
Scutellaria baicalensis (Baikal skullcap) has been used for centuries in traditional Chinese medicine (TCM). The root of the Baikal skullcap (Scutellariae baicalensis radix) is also a recognized raw material in European therapeutic practice. It is mentioned in European Pharmacopoeia since 2010 and also in the WHO monograph. This raw material is a rich source of specyfic flavonoids, especially from the group of flavones, such as baicalein, baicalin, wogonin, and wogonoside. These compounds show a number of valuable biological activities.
The aim of the presented research was to propose in vitro cultures of Scutellaria baicalensis as an alternative to the raw material obtained from cultivated plants for providing apart from flavonoids also phenylpropanoid glycosides and phenolic acids. Standard biotechnological treatments, such as a selection of the composition of the basal medium, selection of the concentration and mutual proportions of plant growth regulators, and different types of culture cultivation (solid, agitated and bioreactor’s cultures), were used. In addition, elicitation and feeding with biosynthetic precursors were tested.
The optimization of culturing conditions led to increased accumulation of species-specific flavonoids. Additionally in vitro cultures proved to be a particularly rich source of the phenylpropanoid glycoside - verbascoside.
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Abbreviations
- BAP:
-
6-Benzylaminopurine
- DW:
-
Dry weight
- HPLC:
-
High-Performance Liquid Chromatography
- LS:
-
Linsmaier and Skoog
- MS:
-
Murashige and Skoog
- NAA:
-
α-Naphthaleneacetic acid
- PGRs:
-
Plant Growth Regulators
- TCM:
-
Traditional Chinese Medicine
- WHO:
-
World Health Organization
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Kawka, B., Kwiecień, I., Ekiert, H.M. (2021). Production of Specific Flavonoids and Verbascoside in Shoot Cultures of Scutellaria baicalensis. In: Ramawat, K.G., Ekiert, H.M., Goyal, S. (eds) Plant Cell and Tissue Differentiation and Secondary Metabolites. Reference Series in Phytochemistry. Springer, Cham. https://doi.org/10.1007/978-3-030-30185-9_7
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