Summary
The study of medicinal plants has many unique challenges and special considerations. These plants are studied for their specific chemistry, or pharmacologic activity. Plants are highly sensitive to their environment and respond through changes in their chemistry. To date, one of the biggest problems in the study of medicinal plants has been the acquisition of consistent, positively identified material for chemical analysis. Successful protocols for the collection, identification, and establishment of medicinal plants species in tissue culture is invaluable for future studies. This protocol outlines methods to establish Scutellaria baicalenisis, and Scutellaria lateriflora from commercial seed sources, and collection and establishment of Scutellaria racemosa from wild populations.
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References
Paton, A. (1990). A global investigation of Scutellaria (Labiatae). Kew Bull. 45, 399–450.
Watanabe, H., Kobayashi, T., Meibou, T., Sekiguchi, Y., Uchida, K., Aoki, T., and Cyong, J.C. (2002). Effects of Kampo herbal medicine on plasma melatonin in murine sarcoma S 180 both in vitro and in vivo. Biol Pharm. Bull. 29, 1132–1137.
Murch, S.J., Rupasinghe, H.P.V., Goodenowe, D., and Saxena, P.K. (2004). A metobolomic analysis of medicinal diversity in Huang-qin (Scutellaria baicalensis Georgi.) genotypes discovery of novel compounds. Plant Cell Rep. 23, 419–425.
Hsieh, T.C., Lu, X., Chea, J., and Wu, J.M. (2002). Prevention and management of prostate cancer using PC-SPES: a scientific perspective. J Nutrition. 132, 3513–3517.
Chang, W.T., Chem, C.H., and Lu, F.J. (2002). Different effects of baicalein, baicalin and wogonin on mitochondrial function, glutathione content and cell cycle progression in human hepatoma cell lines. Planta Medica. 68, 128–132.
Li, B.Q., Fu, T., Yan, Y.D., Baylor, N.W., Ruscetti, F.W., and Kung, H. (1993). Inhibition of HIV infection by baicalin-a flavonoid compound purified from Chinese herbal medicine. Cell Mol Biol Res. 339, 119–124.
Li, F.Q., Wang, T., Pei, Z., Liu, B., and Hong, J.S. (2004). Inhibition of microglial activation by the herbal flavonoid baicalein attenuates inflammation-mediated degeneration of dopaminergic neurons. J Neur Trans. 112, 331–347.
de Boer, J.G., Quiney, B., Walter, P.B., Thomas, C., Hodgson, K., Murch, S.J., and Saxena, P.K. (2005.) Protection against aflatoxin-B1-induced liver mutagenesis by Scutellaria baicalensis Mut. Res. 578, 1–22.
Nan, J.X., Park, E.J., Kim, Y.C., Ko, G., and Sohn, D.H. (2002). Scutellaria baicalensis inhibits liver fibrosis induced by bile duct litigation on carbon tetrachloride in rats. J Pharm Pharmaco. 54, 555–563.
Zhao, Y., Li, H., Gao, Z., Gong, Y., and Xu, H. (2000). Effects of flavonoids extracted from Scutellaria baicalensis Georgi. On hemin-nitrite-H2O2 induced liver injury. Eu J Pharm. 536, 192–199.
Lim, B.O. (2003). Effects of wogonin, wogonoside and 3,5,7,2′,6′-pentahydroxyflavone on chemical mediator production in peritoneal excudate cells and immunoglobin E of rat mesenteric lymph node lymphocytes. J Ethnopharm. 84, 23–29.
Moerman, D.E. (1998). Native American Ethnobotany. Timber Press, Portland, OR.
Johnson, T. (1999). CRC Ethnobotany Reference. CRC, Boca Raton, FL.
Foster, S. and Tyler, V.E. (1999). Tyler’s honest herbal. New York, Haworth Herbal Press, NJ.
Gafner, S.C., Bergeron, L.L., Batcha, J., Reich, J., Arnason, J.T., Burdette, J.E., Pezzuto, J.M., and Angerhofer, C.K. (2003). Inhibition of [3H]-LSD binding to 5-HT7 receptors by flavonoids from Scutellaria lateriflora. J Nat Prod. 66, 535–537.
Awad, R., Arnason, J.T., Trudeau, V., Bergeron, C., Budzinski, J.W., Foster, B.C., and Merali, Z. (2003). Phytochemical and Biological analysis of Skullcap (Scutellaria laterifolia L.): a medicinal plant with anxiolytic properties. Phytomed. 10, 640–649.
Kral, R. (1973). Some notes on the flora of the southern states, particularly Alabama and Tennessee. Rhodora. 75, 366–410.
Krings, A. and Neal, J.C. (2001). South American Skullcap (Scutellaria racemosa: Lamiaceae) in the Southeastern United States. SIDA. 19, 1171–1179.
Bianchi, A. (2006). Attivita antidepressiva di due specie di Scutellariae Colombiane. VI Convegno AMIAR. Torino, Italy.
Murch, S.J., KrishnaRaj, S., and Saxena P.K. (2000). Phytopharmaceuticals: Problems, Limitations and Solutions. Sci Rev Alt Med. 4, 33–38.
Saxena, P.K., Cole, I.B., and Murch, S.J. (2007). Approaches to quality plant based medicine: Significance of chemical profiling. In: Applications of plant metabolic engineering. (Verpoote, R., ed.), Springer, NY, pp. 303–323.
Murch, S.J., KrishnaRaj, S., and Saxena, P.K. (2000) Phytopharmaceuticals:Mass-Production, Standardization and Conservation. Sci Rev Alt Med. 4, 39–43.
Zobayed, S.M.A., Murch, S.J., Rupasinghe, H.P.V., de Boer, J.G., Glickman, B.W., and Saxena, P.K. (2004). Optimized system for biomass production, chemical characterization and evaluation of chemo-preventive properties of Scutellaria baicalensis Georgi. Plant Sci. 167, 439–446.
Cole, I.B., Saxena, P.K., and Murch, S.J. (2007). Medicinal biotechnology in the genus Scutellaria. In Vitro Cell. Dev. Biol. Plant. 43, 318–327.
Pool, A. (2006). New species of Scutellaria (Lamiaceae) from Mesoamerica. Novon. 16, 388–403.
Wunderlin, R. P., and B. F. Hansen. (2004). Atlas of Florida Vascular Plants (http://www.plantatlas.usf.edu/). (Landry, S. M. and Campbell, K. N., ed.), Florida Center for Community Design and Research. Institute for Systematic Botany, University of South Florida, Tampa, FL.
Bridson D. and Forman, L. (2004). The Herbarium Handbook. The Royal Botanic Gardens, Kew Press, Richmond, BC.
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Cole, I.B., Farooq, F.T., Murch, S.J. (2009). Protocols for Establishment of an In Vitro Collection of Medicinal Plants in the Genus Scutellaria. In: Jain, S.M., Saxena, P.K. (eds) Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants. Methods in Molecular Biology, vol 547. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-287-2_13
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DOI: https://doi.org/10.1007/978-1-60327-287-2_13
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