Abstract
There are four Eryngium species native to Poland, namely, E. planum and E. campestre – rare taxa, E. maritimum and E. alpinum – endangered and protected taxa belonging to the subfamily Saniculoideae of Apiaceae family. The phytochemical investigations revealed the presence of various groups of bioactive compounds, that is, triterpenoid saponins, phenolic acids, flavonoids, coumarin derivatives, the essential oil, polyacetylenes, phytosterols, and ecdysteroids. Plant in vitro cultures of those rare and endangered species as well as biotechnological methods of application may provide biomass with the enhanced accumulation of desired secondary metabolites without collecting plants from natural sites. Protocols of micropropagation with genome size stability confirmation and different types of cultures – organ, callus, and cell suspension cultures – were developed with the intent to achieve constant, uniform, and renewable biomass with the higher accumulation of polyphenols and triterpenoid saponins. Both soil-grown plants and in vitro systems were analyzed for the presence and content of main secondary metabolites. It was shown that in vitro-derived plantlets also showed biological activities. The phytochemical and biological studies of Eryngium species show their potential as valuable medicinal plants.
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Abbreviations
- 2,4-D:
-
2,4-Dichlorophenoxyacetic acid
- ABTS:
-
2,2′-Azinobis-3-ethylbenzotiazo-line-6-sulfonic acid
- AChE:
-
Acetylcholinesterase
- BAP:
-
Benzylaminpurine
- BuChE:
-
Butyrylcholinesterase
- CA:
-
Caffeic acid
- CGA:
-
Chlorogenic acid
- DPPH:
-
2,2-Diphenyl-1-picrylhydrazyl
- DW:
-
Dry weight
- EC50:
-
Half maximal effective concentration
- ES1:
-
3-O-β-d-Glucopyranosyl-(1→2)-β-d-glucuronopyranosyl-21-O-acetyl,22-O-angeloyl-R1-barrigenol
- ES2:
-
3-O-β-d-Glucopyranosyl-(1→2)-β-d-glucuronopyranosyl-22-O-angeloyl-R1-barrigenol
- ES3:
-
3-O-β-d-Glucopyranosyl-(1→2)-β-d-glucuronopyranosyl-22-O-angeloyl-A1-barrigenol
- GA3:
-
Gibberellic acid
- GC:
-
Gas chromatography
- GC-FID-MS:
-
Gas chromatography-flame ionization-mass spectrometer
- GC-MS:
-
Gas chromatography-mass spectrometer
- HPLC:
-
High performance liquid chromatography
- HPLC-DAD:
-
High performance liquid chromatography – diode-array detector
- IAA:
-
Indole-3-acetic acid
- IBA:
-
Indole-3-butyric acid
- IC50:
-
Half maximal inhibitory concentration
- KIN:
-
Kinetin
- LC-MS:
-
Liquid chromatography-mass spectrometer
- MeJa:
-
Methyl jasmonate
- MIC:
-
Minimal inhibitory concentration
- MS:
-
Murashige and Skoog medium
- NAA:
-
Naphthaleneacetic acid
- NMR:
-
Nuclear magnetic resonance
- RA:
-
Rosmarinic acid
- TAC:
-
Total antioxidant capacity
- TBA:
-
Thiobarbituric acid
- UHPLC:
-
Ultra-high performance liquid chromatography
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Kikowska, M., Thiem, B. (2020). In Vitro Systems of Selected Eryngium Species (E. planum, E. campestre, E. maritimum, and E. alpinum) for Studying Production of Desired Secondary Metabolites (Phenolic Acids, Flavonoids, Triterpenoid Saponins, and Essential Oil). In: Ramawat, K., Ekiert, H., Goyal, S. (eds) Plant Cell and Tissue Differentiation and Secondary Metabolites. Reference Series in Phytochemistry. Springer, Cham. https://doi.org/10.1007/978-3-030-11253-0_29-2
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) for Studying Production of Desired Secondary Metabolites (Phenolic Acids, Flavonoids, Triterpenoid Saponins, and Essential Oil)- Published:
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DOI: https://doi.org/10.1007/978-3-030-11253-0_29-2
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