Abstract
As the cellular component of innate immunity, macrophages directly challenge invading pathogens by secreting pro-inflammatory cytokines and initiating a protective inflammatory response. However, bacterial pathogens have evolved sophisticated strategies for invading or counteracting the host defenses. The survival and replication of Yersinia pestis in macrophages is likely to be of great importance at the early stage of infection. That macrophages can serve as permissive sites for bacterial replication is supported by both in vivo and in vitro experiments. Thus, the study of the interaction between Y. pestis and macrophages will increase our understanding of how Yersinia subverts the host immune response. Here I describe the isolation and culture of mice macrophages, the infection of macrophages with Y. pestis, and the detection of infected macrophage functions. While I focus on the interactions between Y. pestis and macrophages, the describe methods, with some adaptations, may be suitable for analyzing other bacterial species.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Perry RD, Fetherston JD (1997) Yersinia pestis – etiologic agent of plague. Clin Microbiol Rev 10:35–66
Straley SC, Harmon PA (1984) Growth in mouse peritoneal macro- phages of Yersinia pestis lacking established virulence determinants. Infect Immun 45:649–654
Janssen WA, Surgalla MJ (1968) Plague bacillus: survival within host phagocytes. Science 163:950–952
Gordon S (2004) Pathogen recognition or homeostasis? APC receptor functions in innate immunity. C R Biol 327:603–607
Cailhier JF, Partolina M, Vuthoori S, Wu S, Ko K, Watson S, Savill J, Hughes J, Lang RA (2005) Conditional macrophage ablation demonstrates that resident macrophages initiate acute peritoneal inflammation. J Immunol 174:2336–2342
Kaufmann SH, Schaible UE (2005) Antigen presentation and recognition in bacterial infections. Curr Opin Immunol 17:79–87
Bi Y, Du Z, Han Y, Guo Z, Tan Y, Zhu Z, Yang R (2009) Yersinia pestis and host macrophages: immunodeficiency of mouse macrophages induced by YscW. Immunology 128(1 Suppl):e406–e417
Bi Y, Du Z, Yang H, Guo Z, Tan Y, Zhu Z, Yang R (2009) Reduced apoptosis of mouse macrophages induced by yscW mutant of Yersinia pestis results from the reduced secretion of YopJ and relates to caspase-3 signal pathway. Scand J Immunol 70(4):358–367
Geng J, Song Y, Yang L, Feng Y, Qiu Y, Li G, Guo J, Bi Y, Qu Y, Wang W et al (2009) Involvement of the post-transcriptional regulator Hfq in Yersinia pestis virulence. PLoS One 4(7):e6213
Zakharova M, Ziegler HK (2005) Paradoxical anti-inflammatory actions of TNF-alpha: inhibition of IL-12 and IL-23 via TNF receptor 1 in macrophages and dendritic cells. J Immunol 175:5024–5033
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Nature Singapore Pte Ltd.
About this protocol
Cite this protocol
Bi, Y. (2018). Effects of Y. pestis on Macrophages. In: Yang, R. (eds) Yersinia Pestis Protocols. Springer Protocols Handbooks. Springer, Singapore. https://doi.org/10.1007/978-981-10-7947-4_33
Download citation
DOI: https://doi.org/10.1007/978-981-10-7947-4_33
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-10-7946-7
Online ISBN: 978-981-10-7947-4
eBook Packages: Springer Protocols