Abstract
Knowing that human embryonic stem cells (HESC) can be derived into several different cells types render these cells very attractive to cure diseases. Unless these stem cells are originated from the patient itself, they will be isolated from a donor, who is genetically unrelated to the recipient. This situation will mimic an allogenic transplantation with an immune response against the transplanted cells. The immunogenicity of the HESC and the potential of NK and T-cells to target HESC and the lineage derived from HESC have to be addressed. Several different tests do exist to analyse NK cells and T-cells activity against HESC and its progenitor cells. In this chapter review the capacity of NK and T cells against neural progenitor derived from HESC, through a classical and a novel approach that combined the phenotype and also the functionality of the effector cells. In addition, we also demonstrate in the same test that we can determine the lysis of the progenitor cells by flow cytometry.
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Abbreviations
- 7-AAD:
-
7-Aminoactinomycin
- CFSE:
-
5-Carboxyfluorescein diacetate succinimidyl ester
- FACS:
-
Flow cytometry
- HESC:
-
Human embryonic stem cells
- NK cells:
-
Natural killer cells
- NPC:
-
Neuronal progenitor cells
References
Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS et al (1998) Embryonic stem cell lines derived from human blastocysts. Science 282(5391):1145–1147
Pera MF, Cooper S, Mills J, Parrington JM (1989) Isolation and characterization of a multipotent clone of human embryonal carcinoma cells. Differentiation 42(1):10–23
Morizane A, Li JY, Brundin P (2008) From bench to bed: the potential of stem cells for the treatment of Parkinson’s disease. Cell Tissue Res 331(1):323–336
Cooper MA, Fehniger TA, Caligiuri MA (2001) The biology of human natural killer-cell subsets. Trends Immunol 22(11):633–640
Farag SS, Fehniger TA, Ruggeri L, Velardi A, Caligiuri MA (2002) Natural killer cell receptors: new biology and insights into the graft-versus-leukemia effect. Blood 100(6):1935–1947
Ljunggren HG, Karre K (1990) In search of the ‘missing self’: MHC molecules and NK cell recognition. Immunol Today 11(7):237–244
Alter G, Malenfant JM, Altfeld M (2004) CD107a as a functional marker for the identification of natural killer cell activity. J Immunol Methods 294(1–2):15–22
Preynat-Seauve O, de Rham C, Tirefort D, Ferrari-Lacraz S, Krause KH, Villard J (2009) Neural progenitors derived from human embryonic stem cells are targeted by allogeneic T and natural killer cells. J Cell Mol Med 13:3556–3569
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de Rham, C., Villard, J. (2013). Interaction of ES Cell Derived Neural Progenitor Cells with Natural Killer Cells and Cytotoxic T Cells. In: Zavazava, N. (eds) Embryonic Stem Cell Immunobiology. Methods in Molecular Biology, vol 1029. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-478-4_5
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DOI: https://doi.org/10.1007/978-1-62703-478-4_5
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-477-7
Online ISBN: 978-1-62703-478-4
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