Abstract
Plant phospholipids and glycolipids can be analyzed by direct infusion electrospray ionization triple-quadrupole mass spectrometry. A biological extract is introduced in solvent by continuous infusion into the mass spectrometer’s electrospray ionization source, where ions are produced from the lipids. For analysis of membrane lipids, a series of precursor and neutral loss scans, each specific for lipids containing a common head group, are obtained sequentially. The mass spectral data are processed and combined, using the Web application LipidomeDB Data Calculation Environment, to create a lipid profile.
Sunitha Shiva and Hieu Sy Vu have contributed equally.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Brügger B et al (1997) Quantitative analysis of biological membrane lipids at the low picomole level by nano-electrospray ionization tandem mass spectrometry. Proc Natl Acad Sci USA 94:2339–2344
Taguchi R et al (2005) Focused lipidomics by tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci 823:26–36
Isaac G et al (2007) New mass spectrometry-based strategies for lipids. Genet Eng (N Y) 28:129–157
Welti R et al (2002) Profiling membrane lipids in plant stress responses: role of phospholipase D-α in freezing-induced lipid changes in Arabidopsis. J Biol Chem 277:31994–32002
Devaiah SP et al (2006) Quantitative profiling of polar glycerolipid species and the role of phospholipase Dα1 in defining the lipid species in Arabidopsis tissues. Phytochemistry 67:1907–1924
Comfurius P, Zwaal RF (1977) The enzymatic synthesis of phosphatidylserine and purification by CM-cellulose column chromatography. Biochim Biophys Acta 488:36–42
Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Physiol Pharmacol 37:911–917
Ames BN (1966) Assay of inorganic phosphate, total phosphate and phosphatases. In: Neufeld E, Ginsburg V (eds) Methods in enzymology: complex carbohydrates, vol VIII. Academic, New York, pp 115–118
Markham JE et al (2006) Separation and identification of major plant sphingolipid classes from leaves. J Biol Chem 281:22684–22694
Acknowledgments
The development of plant lipidomics methodology was supported by the National Science Foundation (MCB 0455318 and MCB 0920663) to R.W., Xuemin Wang, Jyoti Shah, Todd Williams, and Gary Gadbury. We are very grateful to these, other colleagues, and lab members who have contributed to plant lipidomics development. Contribution no. 11-300-B from the Kansas Agricultural Experiment Station.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Shiva, S. et al. (2013). Lipidomic Analysis of Plant Membrane Lipids by Direct Infusion Tandem Mass Spectrometry. In: Munnik, T., Heilmann, I. (eds) Plant Lipid Signaling Protocols. Methods in Molecular Biology, vol 1009. Humana, Totowa, NJ. https://doi.org/10.1007/978-1-62703-401-2_9
Download citation
DOI: https://doi.org/10.1007/978-1-62703-401-2_9
Published:
Publisher Name: Humana, Totowa, NJ
Print ISBN: 978-1-62703-400-5
Online ISBN: 978-1-62703-401-2
eBook Packages: Springer Protocols