Abstract
Lipid analysis performed by nano-electrospray ionization mass spectrometry is a highly sensitive method for quantification of lipids including all lipid species of a given lipid class. Various instrumental setups are used for quantitative lipid analysis, including different modes of ionization, separation, and detection. Here we describe a work-flow for the rapid and quantitative analysis of lipid species from cellular membranes by direct infusion of lipid extracts to a nano-electrospray ionization triple quadrupole/linear ion trap mass spectrometer.
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Acknowledgment
This work was supported by a grant of the Deutsche Forschungsgemeinschaft (SFB/TRR83). B.B. is an investigator of the CellNetworks Cluster of Excellence (EXC81).
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Özbalci, C., Sachsenheimer, T., Brügger, B. (2013). Quantitative Analysis of Cellular Lipids by Nano-Electrospray Ionization Mass Spectrometry. In: Rapaport, D., Herrmann, J. (eds) Membrane Biogenesis. Methods in Molecular Biology, vol 1033. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-487-6_1
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DOI: https://doi.org/10.1007/978-1-62703-487-6_1
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