Abstract
In this chapter the methodological bases are provided to achieve subnanometer resolution on two-dimensional (2D) membrane protein crystals by atomic force microscopy (AFM). This is outlined in detail with the example of AFM studies of the outer membrane protein F (OmpF) from the bacterium Escherichia coli (E. coli). We describe in detail the high-resolution imaging of 2D OmpF crystals in aqueous solution and under near-physiological conditions. The topographs of OmpF, and stylus effects and artifacts encountered when imaging by AFM are discussed.
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Acknowledgments
We are grateful to Andreas Engel for his continous support. This work was supported by the Swiss National Foundation for Scientific Research (Grant 31003A_125150 to D.F.), the National Center of Competence in Research (NCCR) TransCure, and the European Science Foundation (Grant 09-EuroSYNBIO-FP-012 NANOCELL to D.F. and D.J.M.).
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Fotiadis, D., Müller, D.J. (2013). High-Resolution Imaging of 2D Outer Membrane Protein F Crystals by Atomic Force Microscopy. In: Schmidt-Krey, I., Cheng, Y. (eds) Electron Crystallography of Soluble and Membrane Proteins. Methods in Molecular Biology, vol 955. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-176-9_24
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DOI: https://doi.org/10.1007/978-1-62703-176-9_24
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