Abstract
Atomic force microscopy (AFM) is a very versatile tool for studying biological samples at nanometer-scale resolution. The cell membrane plays a key role in compartmentalization, nutrient transportation, and signal transduction, while the structural feature of both sides of the membrane remains elusive. Here we describe our methods for the preparation of the cell membrane from the red blood cells and nucleated cells. High-resolution AFM topographs reveal substructural details of both sides of the cell membrane. The structure composition of cell membrane can be directly observed by time-lapse AFM and the positional information of membrane proteins can be located by molecular recognition.
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Acknowledgments
This work was supported by National Key R&D Program of China (No.2017YFA0505300), and National Natural Science Foundation of China (No. 21525314, 21503213, 21727816 and 21721003).
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Cai, M., Wang, H. (2019). Atomic Force Microscopy for Cell Membrane Investigation. In: Weissig, V., Elbayoumi, T. (eds) Pharmaceutical Nanotechnology. Methods in Molecular Biology, vol 2000. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9516-5_22
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DOI: https://doi.org/10.1007/978-1-4939-9516-5_22
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