Abstract
Many human embryonic stem (hES) and induced pluripotent stem (hiPS) cell differentiation protocols begin with the formation of three-dimensional aggregates of cells called embryoid bodies (EBs). Traditional EB formation methods result in a heterogeneous population of EB sizes and shapes, which then undergo heterogeneous differentiation efficiencies. AggreWellTM400 and AggreWellTM800 use the spin-EB method to force the aggregation of a defined number of cells, thereby controlling EB size and generating a population of uniform EBs. Moreover, the dense array of microwells on the bottom surface of AggreWellTM400 provide for the rapid and simple production of thousands of EBs at a time.
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Antonchuk, J. (2013). Formation of Embryoid Bodies from Human Pluripotent Stem Cells Using AggreWell™ Plates. In: Helgason, C., Miller, C. (eds) Basic Cell Culture Protocols. Methods in Molecular Biology, vol 946. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-128-8_32
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DOI: https://doi.org/10.1007/978-1-62703-128-8_32
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-127-1
Online ISBN: 978-1-62703-128-8
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