Abstract
Immunoblotting is a core method in molecular neuroscience to detect and/or quantify single proteins in a complex mixture. The whole procedure covers the solubilization of proteins, their separation by mass on an SDS-polyacrylamide gel, the electrophoretic transfer to a membrane, and the immunodetection of the target proteins by using specific antibodies. Here we provide protocols for SDS-PAGE followed by electroblotting combined with immunostaining. Useful hints are included for method optimization.
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Klemmer, P. (2011). SDS-PAGE Immunoblot Analysis. In: Li, K. (eds) Neuroproteomics. Neuromethods, vol 57. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-111-6_13
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DOI: https://doi.org/10.1007/978-1-61779-111-6_13
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-110-9
Online ISBN: 978-1-61779-111-6
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