Abstract
The ability to detect transient changes in molecular networks lies at the heart of cancer biology research. This is especially apparent during tumorigenesis, where initiating mutations typically affect mitogens and cell-cycle molecules such as PDGF or retinoblastoma protein (Rb). One of the primary consequences of such processes is the inappropriate stimulation of downstream targets, normally through posttranslational modification. Immunohistochemistry (IHC) provides an important tool for assessing such changes in situ, permitting different aspects of tumor biology to be examined as a tissue undergoes transformation. Nevertheless, this can be difficult to achieve, particularly in complex environments like the brain. Here, we provide the automated methodology we have employed for the successful detection of phosphorylation of S6 ribosomal protein (S6-RP) and the retinoblastoma protein (Rb) in response to PDGF stimulation in a mouse model of glial brain tumor development.
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References
Holland, E.C. (2001) Gliomagenesis: genetic alterations and mouse models. Nature Genetics 2, 120–129.
Dai, C., Celestino, J.C., Okada, Y., Louis, D.N., Fuller, G.N., and Holland, E.C. (2001) PDGF autocrine stimulation dedifferentiates cultured astrocytes and induces oligodendrogliomas and oligoastrocytomas from neural progenitors and astrocytes in vivo. Genes and Development 15, 1913–1925.
Acknowledgments
We acknowledge the hard work of everyone in the Molecular Cytology Core Facility at Memorial Sloan-Kettering Cancer Center for helping make our experiments just that little bit easier. We also thank Massimo Squattiro (MSKCC) for allowing us to use some of his results in this protocol chapter.
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© 2011 Springer Science+Business Media, LLC
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Ciznadija, D., Barlas, A., Manova, K. (2011). Immunohistochemical Assessment of Signal Transduction and Cell-Cycle Networks in Neural Tumors. In: Kalyuzhny, A. (eds) Signal Transduction Immunohistochemistry. Methods in Molecular Biology, vol 717. Humana Press. https://doi.org/10.1007/978-1-61779-024-9_12
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DOI: https://doi.org/10.1007/978-1-61779-024-9_12
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