Summary
Imaging mass spectrometry (IMS) provides a unique method to probe for chemical distributions within tissue sections with high chemical specificity. The direct analysis of tissue sections by mass spectrometry, which is the field of IMS, is relatively young, 10 year old; however, the techniques for mass spectrometric analysis are well known. Critical aspects of IMS then are the preparation of tissue specimens for insertion into a vacuum chamber and the interpretation of results with respect to disease studies. Here, we describe the methodologies for geographic localization of phospholipids in flat-mounted eye segments from rhesus monkey using IMS with matrix-assisted laser desorption/ionization (MALDI) and tandem mass spectrometry.
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References
Abdelsalam, A., Del Priore, L., and Zarbin, M. A. (1999) Drusen in age-related macular degeneration: pathogenesis, matural course and laser photocoagulation induced regression, Survey Ophthal. 44, 1–29.
Donoso, L. A., Kim, D., Frost, A., Callahan, A., and Hageman, G. (2006) The role of inflammation in this pathogenesis of age-related macular disease, Survey of Ophthal. 51, 137–152.
Anderson, M., Dawson, W. W., Gonzalez-Martinez, J., and Curcio, C. A. (2006) Drusen and lipid-filled retinal pigment epithelium cells in a rhesus macula, Vet. Opthal. 9, 201–207.
Curcio, C. A., Presley, J. B., Millican, C. L., and Medeiros, N. E. (2005) Basal deposits and drusen in eyes with age-related maculopathy: evidence for solid lipid particles, Exp. Eye Res. 80, 761–775.
Garrett, T. J., Dawson, W., and Yost, R. A. (2007) ASMS Conference on Mass Spectrometry and Allied Topics, Indianapolis, IN.
Caprioli, R. M., Farmer, T. B., and Gile, J. (1997) Molecular imaging of biological samples: Localization of peptides and proteins using MALDI-TOF MS, Anal. Chem. 69, 4751–4760.
Chaurand, P., and Caprioli, R. M. (2002) Direct profiling and imaging of peptides and proteins from mammalian cells and tissue sections by mass spectrometry, Electrophoresis 23, 3125–3135.
Chaurand, P., Schwartz, S. A., and Caprioli, R. M. (2004) Assessing protein patterns in disease using imaging mass spectrometry, J. Proteome Res. 3, 245–252.
McDonnell, L. A., Piersma, S. R., Altelaar, A. F. M., Mize, T. H., Luxembourg, S. L., Verhaert, P. D. E. M., van Minnen, J., and Heeren, R. M. A. (2005) Subcellular imaging mass spectrometry of brain tissue, J. Mass Spectrom. 40, 160–168.
Monroe, E. B., Jurchen, J. C., Lee, J., Rubakhin, S. S., and Sweedler, J. V. (2005) Vitamin E imaging and localization in the neuronal membrane, J. Am. Chem. Soc. 127, 12152–12153.
Chaurand, P., Schwartz, S. A., Billheimer, D., Xu, B. J., Crecelius, A., and Caprioli, R. M. (2004) Integrating histology and imaging mass spectrometry, Anal. Chem. 76, 1145–1155.
Maddalo, G., Petrucci, F., Iezzi, M., Pannellini, T., Del Boccio, P., Ciavardelli, D., Biroccio, A., Forli, F., Di Ilio, C., Ballone, E., Urbani, A., and Federici, G. (2005) Analytical assessment of MALDI-TOF imaging mass spectrometry on this histological samples. An insight into proteome investigation, Clin. Chim. Acta 357, 210–218.
Reyzer, M. L., Hsieh, Y., Ng, K., Korfmacher, W. A., and Caprioli, R. M. (2003) Direct analysis of drug candidates in tissue by matrix-assisted laser desorption/ionization mass spectrometry, J. Mass Spectrom. 38, 1081–1092.
Jackson, S. N., Wang, H.-Y. J., and Woods, A. S. (2005) In situ structural characterization of phosphatidylcholines in brain tissue using MALDI-MS/MS, J. Am. Soc. Mass Spectrom. 16, 2052–2056.
Garrett, T. J., and Yost, R. A. (2009, submitted to Methods in Molecular Biology) Characterization of phospholipids in rat brain tissue by imaging tandem mass spectrometry with intermediate-pressure MALDI on a linear ion trap.
Drexler, D. M., Garrett, T. J., Cantone, J. L., Diters, R. W., Mitroka, J. G., Prieto-Conaway, M. C., Adams, S. P., Yost, R. A., and Sanders, M. (2007) Utility of imaging mass spectrometry (IMS) by matrix-assisted laser desoption ionization (MALDI) on an ion trap mass spectrometer in the analysis of drugs and metabolites in biological tissues, J. Pharm. and Toxicol. Methods 55, 279–288.
Stoeckli, M., Chaurand, P., Hallahn, D. E., and Caprioli, R. M. (2001) Imaging mass spectrometry: A new technology for the analysis of protein expression in mammalian tissues, Nature Med. 7, 493–496.
Winograd, N. (2003) Prospects for imaging TOF-SIMS: from fundamentals to biotechnology, Appl Surface Sci. 203–204, 13–19.
Todd, P. J., Schaaf, T. G., Chaurand, P., and Caprioli, R. M. (2001) Organic ion imaging of biological tissue with secondary ion mass spectrometry and matrix-assisted laser desorption/ionization, J. Mass Spectrom. 36, 355–369.
Todd, P. J., Schaaf, T. G., Chaurand, P., and Caprioli, R. M. (2001) Organic ion imaging of biolocial tissue with secondary ion mass spectrometry and matrix-assisted laser desorption/ionization, J. Mass Spectrom. 36, 355–369.
Garrett, T. J. (2006) Chemistry, pp. 148, University of Florida, Gainesville, FL.
Garrett, T. J., Kovtoun, V. V., Buy, H., Izgarian, N., Prieto-Conaway, M. C., Stafford, G., and Yost, R. A. (2007) Imaging mass spectrometry with a new intermediate-pressure MALDI linear ion trap mass spectrometer, Int. J. Mass Spectrom. 263, 166–176.
Cha, S., and Yeung, E. S. (2007) Colloidal graphite-assisted laser desorption/ionization mass spectrometry and MSn of small molecules. 1. Imaging of cerebrosides directly from rat brain tissue, Anal. Chem. 79, 2373–2385.
Garrett, T. J., Prieto-Conaway, M. C., Kovtoun, V., Bui, H., Izgarian, N., Stafford, G. C., and Yost, R. A. (2007) Imaging of small molecules in tissue sections with a new intermediate-pressure MALDI linear ion trap mass spectrometer, Int. J. Mass Spectrom. 260, 166–176.
Jackson, S. N., Wang, H.-Y. J., and Woods, A. S. (2005) Direct profiling of lipid distribution in brain tissue using MALDI-TOFMS, Anal. Chem. 77, 4523–4527.
Han, X., and Gross, R. W. (1995) Structural determination of picomole amounts of phospholipids via electrospray ionization tandem mass spectrometry, J. Am. Soc. Mass Spectrom. 6, 1201–1210.
Hsu, F.-F., Bohrer, A., and Turk, J. (1997) Formation of lithiated adducts of glycerophosphocholine lipids facilitates their identification by electrospray ionization tandem mass spectrometry, J. Am. Soc. Mass Spectrom. 9, 516–526.
Dawson, W. W., Dawson, J. L. K., and Gonzalez-Martinez, J. (2008) Maculas, monkeys, models, AMD, and aging, Vis. Res. 48, 360–365.9
Ho, Y.-P., Huang, P.-C., and Deng, K.-H. (2003) Metal ion complexes in the structural analysis of phospholipids by electrospray ionization tandem mass spectrometry, Rapid Commun. Mass Spectrom. 17, 114–121.
Stone, J. (1981) The whole mount handbook, Maitland Publications, Sydney.
Acknowledgments
The authors thank the Lion’s Eye Bank of Tampa, FL for donation of human tissue specimens. The Lion’s Club International Foundation of Jacksonville, FL is greatly acknowledged for funding.
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Garrett, T.J., Dawson, W.W. (2009). Lipid Geographical Analysis of the Primate Macula by Imaging Mass Spectrometry. In: Armstrong, D. (eds) Lipidomics. Methods in Molecular Biology, vol 579. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-322-0_12
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DOI: https://doi.org/10.1007/978-1-60761-322-0_12
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