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Analyses and Localization of Phosphatidylcholine and Phosphatidylserine in Murine Ocular Tissue Using Imaging Mass Spectrometry

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Lipidomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2625))

Abstract

Imaging mass spectrometry (IMS) allows for spatial visualization of proteins, lipids, and metabolite distributions in a tissue. Identifying these compounds through mass spectrometry, combined with mapping the compound distribution in the sample in a targeted or untargeted approach, renders IMS a powerful tool for lipidomics. IMS analysis for lipid species such as phosphatidylcholine and phosphatidylserine allows researchers to pinpoint areas of lipid deficiencies or accumulations associated with ocular disorders such as age-related macular degeneration and diabetic retinopathy. Here, we describe an end-to-end IMS approach from sample preparation to data analysis for phosphatidylcholine and phosphatidylserine analysis.

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References

  1. Riviera ES, Djambazova KV, Neumann EK et al (2020) Integrating ion mobility and imaging mass spectrometry for comprehensive analysis of biological tissues: a brief review and perspective. J Mass Spectrom 55:e4614. https://doi.org/10.1002/jms.4614

    Article  CAS  Google Scholar 

  2. Desiderio D, Kai M (1983) Preparation of stable isotope-incorporated peptide internal standards for field desorption mass spectrometry quantification of peptides in biologic tissue. Biomed Mass Spectrom 10:471–479. https://doi.org/10.1002/bms.1200100806

    Article  CAS  Google Scholar 

  3. McDonnell LA, Heeren RM (2007) Imaging mass spectrometry. Mass Spectrom 26:606–643. https://doi.org/10.1002/mas.20124

    Article  CAS  Google Scholar 

  4. Zemski Berry KA, Gordon WC, Murphy RC et al (2014) Spatial organization of lipids in the human retina and optic nerve by MALDI imaging mass spectrometry. J Lipid Res 55:504–515

    Article  CAS  Google Scholar 

  5. Spraggins J, Caprioli RM (2011) High-speed MALDI-TOF imaging mass spectrometry: rapid ion image acquisition and considerations for next generation instrumentation. J Am Soc Mass Spectrom 22:1022–1031. https://doi.org/10.1007/s13361-011-0121-0

    Article  CAS  Google Scholar 

  6. Seeley EH, Caprioli RM (2008) Molecular imaging of proteins in tissues by mass spectrometry. Proc Natl Acad Sci 105:18126–18131

    Article  CAS  Google Scholar 

  7. Buchberger AR, DeLaney K, Johnson J et al (2018) Mass spectrometry imaging: a review of emerging advancements and future insights. Anal Chem 90:240–265. https://doi.org/10.1021/acs.analchem.7b04733

    Article  CAS  Google Scholar 

  8. Piehowski PD, Zhu Y, Bramer LM et al (2020) Automated mass spectrometry imaging of over 2000 proteins from tissue sections at 100-μm spatial resolution. Nat Commun 11:8. https://doi.org/10.1038/s41467-019-13858-z

    Article  CAS  Google Scholar 

  9. Singhal N, Kumar M, Kanaujia PK et al (2015) MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis. Front Microbiol 6:791. https://doi.org/10.3389/fmicb.2015.00791

    Article  Google Scholar 

  10. Stoyanovsky DA, Sparvero LJ, Amoscato AA et al (2014) Improved spatial resolution of matrix-assisted laser desorption/ionization imaging of lipids in the brain by alkylated derivatives of 2,5-dihydroxybenzoic acid. Rapid Commun Mass Spectrom 15(28):403–412. https://doi.org/10.1002/rcm.6796

    Article  CAS  Google Scholar 

  11. Murphy RC, Hankin JA, Barkley RM (2009) Imaging of lipid species by MALDI mass spectrometry. J Lipid Res 50:317–322. https://doi.org/10.1194/jlr.R800051-JLR200

    Article  CAS  Google Scholar 

  12. Berry KA, Hankin JA, Barkley RM et al (2011) MALDI imaging of lipid biochemistry in tissues by mass spectrometry. Chem Rev 111:6491–6512. https://doi.org/10.1021/cr200280p

    Article  CAS  Google Scholar 

  13. Li B, Comi TJ, Si T et al (2016) A one-step matrix application method for MALDI mass spectrometry imaging of bacterial colony biofilms. J Mass Spectrom 51:1030–1035. https://doi.org/10.1002/jms.3827

    Article  CAS  Google Scholar 

  14. Leopold J, Popkova Y, Engel KM et al (2018) Recent developments of useful MALDI matrices for the mass spectrometric characterization of lipids. Biomol Ther 8:173. https://doi.org/10.3390/biom8040173

    Article  CAS  Google Scholar 

  15. Karlsson O, Hanrieder J (2017) Imaging mass spectrometry in drug development and toxicology. Arch Toxicol 91:2283–2294. https://doi.org/10.1007/s00204-016-1905-6

    Article  CAS  Google Scholar 

  16. Deutskens F, Yang J, Caprioli RM (2011) High spatial resolution imaging mass spectrometry and classical histology on a single tissue section. J Mass Spectrom 46:568–571. https://doi.org/10.1002/jms.1926

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Bascom Palmer Eye Institute, Miller School of Medicine at University of Miami, Miami, FL, USA

    Varun Krishnan, Sean D. Meehan, Colin Hayter & Sanjoy K. Bhattacharya

  2. Miami Integrative Metabolomics Research Center, Miami, FL, USA

    Varun Krishnan, Sean D. Meehan, Colin Hayter & Sanjoy K. Bhattacharya

  3. University of Miami Miller School of Medicine, Miami, FL, USA

    Varun Krishnan, Sean D. Meehan, Colin Hayter & Sanjoy K. Bhattacharya

Authors
  1. Varun Krishnan
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  2. Sean D. Meehan
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  3. Colin Hayter
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  4. Sanjoy K. Bhattacharya
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Corresponding author

Correspondence to Sanjoy K. Bhattacharya .

Editor information

Editors and Affiliations

  1. Bascom Palmer Eye Institute, University of Miami, Miami, FL, USA

    Sanjoy K. Bhattacharya

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Krishnan, V., Meehan, S.D., Hayter, C., Bhattacharya, S.K. (2023). Analyses and Localization of Phosphatidylcholine and Phosphatidylserine in Murine Ocular Tissue Using Imaging Mass Spectrometry. In: Bhattacharya, S.K. (eds) Lipidomics. Methods in Molecular Biology, vol 2625. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2966-6_14

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  • DOI: https://doi.org/10.1007/978-1-0716-2966-6_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2965-9

  • Online ISBN: 978-1-0716-2966-6

  • eBook Packages: Springer Protocols

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