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Time-Lapse Fluorescence Microscopy of Saccharomyces cerevisiae in Meiosis

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Meiosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 558))

Abstract

Movements are implicit in the chromosome behaviors of bouquet formation, pairing and synapsis during meiotic prophase. In S. cerevisiae, the positions of chromosomes, specific structures, and individual chromosomal loci marked by fluorescent fusion proteins are easily visualized in living cells. Time-lapse analyses have revealed rapid and varied chromosome movements throughout meiotic prophase. To facilitate the analysis of these movements, we have developed a simple, inexpensive, and efficient method to prepare sporulating cells for fluorescence microscopy. This method produces a monolayer of cells that progress from meiosis through spore formation, allows visualization of hundreds of cells in a single high-resolution frame and is suitable for most methods of fluorescence microscopy.

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Acknowledgements

I thank Ben Fowler (Oklahoma Medical Research Foundation Core Facility for Imaging), Margaret Clarke and C.-Y. Lee for advice and technical help in developing the methods. This work was supported by grants NSF #MCB 98-08000 and OCAST #HR07-026.

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Authors and Affiliations

  1. Department of Molecular, Cell and Developmental Biology, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA

    Michael E. Dresser

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  1. Michael E. Dresser
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Editors and Affiliations

  1. Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, York Ave. 1275, New York, 10021-6007, U.S.A.

    Scott Keeney

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Dresser, M.E. (2009). Time-Lapse Fluorescence Microscopy of Saccharomyces cerevisiae in Meiosis. In: Keeney, S. (eds) Meiosis. Methods in Molecular Biology, vol 558. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-103-5_5

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  • DOI: https://doi.org/10.1007/978-1-60761-103-5_5

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-102-8

  • Online ISBN: 978-1-60761-103-5

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