Abstract
Live-cell imaging is widely used by researchers to study cellular dynamics and obtain a deep understanding of cell biological processes. Keeping cells in the proper growing environment and immobilizing the cells are essential for the imaging of live yeast cells. Here we describe a protocol for monitoring cytoophidia in Saccharomyces cerevisiae and Schizosaccharomyces pombe using inverted confocal fluorescence microscopy. This protocol includes yeast culture, sample preparation, fluorescence imaging, and data analysis.
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Acknowledgments
We would like to thank members of the Liu group for discussion and Xiaoming Li from the Molecular Imaging Core Facility of School of Life Science and Technology at ShanghaiTech University for technical supports. We are grateful to Li-Lin Du and the fission yeast community for reagents and discussions. This work was supported by the National Natural Science Foundation of China (11674383 to H.L.), the UK Medical Research Council (to J.L.L), and ShanghaiTech University.
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Zhang, S., Li, H., Liu, JL. (2021). Long-Term Imaging and Dynamic Analysis of Cytoophidia in Yeast. In: Xiao, W. (eds) Yeast Protocols. Methods in Molecular Biology, vol 2196. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0868-5_19
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DOI: https://doi.org/10.1007/978-1-0716-0868-5_19
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-0868-5
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