Abstract
Several technologies have been described to immobilize libraries of small molecules or peptides in a microarray format. Herein, we describe protocols for an alternative strategy whereby each small molecule or peptide within a library is labeled with a peptide nucleic acid (PNA) tag such that they self-assemble in a microarray format upon hybridization with readily available DNA arrays. An important asset of the method is that it allows the library to be used in solution prior to hybridizing and as such offers the opportunity to separate the inhibitors bound to the protein from the rest of the library. Two methods based on size exclusion filtration or gel electrophoresis to separate protein-bound inhibitors from the remaining library are described.
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References
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Acknowledgments
The authors thank the granting agencies which have supported this work (Human Frontier Science Program, Agence National de la Recherche, la Région d’Alsace and the French Ministry of Science). The authors thank their collaborators, in particular Jennifer Harris.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Debaene, F., Winssinger, N. (2009). Self-Assembly of PNA-Encoded Peptides into Microarrays. In: Cretich, M., Chiari, M. (eds) Peptide Microarrays. Methods in Molecular Biology™, vol 570. Humana Press. https://doi.org/10.1007/978-1-60327-394-7_15
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DOI: https://doi.org/10.1007/978-1-60327-394-7_15
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Online ISBN: 978-1-60327-394-7
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