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Micromass Methods for the Evaluation of Developmental Toxicants

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Developmental Toxicology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1965))

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Abstract

Chick embryonic heart has recently been utilized as a model to create a micromass (MM) culturing system. The aim was to overcome the ethical barriers arising from testing the embryotoxicity of chemicals using human embryonic cells. The system represents a valuable tool to study the ability of chemicals to interfere with various embryonic developmental processes such as cellular communication, differentiation, cellular activity, and proliferation, where the disturbance any of them could result in maldevelopment. The system can also be utilized to investigate ROS production and expression of several transmembrane proteins to study their roles in chemical-induced teratogenicity or embryotoxicity.

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Author information

Authors and Affiliations

  1. School of Life Sciences, Faculty of Medicine and Health Sciences, Medical School, QMC, University of Nottingham, Nottingham, NG7 2UH, UK

    Omar J. Mohammed & Margaret K. Pratten

Authors
  1. Omar J. Mohammed
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  2. Margaret K. Pratten
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Corresponding author

Correspondence to Margaret K. Pratten .

Editor information

Editors and Affiliations

  1. Department of Physiology and Developmental Biology, Brigham Young University, Provo, UT, USA

    Jason M. Hansen

  2. Biomedical and Molecular Sciences, Queen’s University, Kingston, ON, Canada

    Louise M. Winn

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Mohammed, O.J., Pratten, M.K. (2019). Micromass Methods for the Evaluation of Developmental Toxicants. In: Hansen, J., Winn, L. (eds) Developmental Toxicology. Methods in Molecular Biology, vol 1965. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9182-2_5

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  • DOI: https://doi.org/10.1007/978-1-4939-9182-2_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9181-5

  • Online ISBN: 978-1-4939-9182-2

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