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Design, Construction, and Application of Transcription Activation-Like Effectors

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Viral Vectors for Gene Therapy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1937))

Abstract

Transcription activator-like effectors (TALEs) are modular proteins derived from the plant Xanthomonas sp. pathogen that can be designed to target unique DNA sequences following a simple cipher. Customized TALE proteins can be used in a variety of molecular applications that include gene editing and transcriptional modulation. Presently, we provide a brief primer on the design and construction of TALEs. TALE proteins can be fused to a variety of different effector domains that alter the function of the TALE upon binding. This flexibility of TALE design and downstream effect may offer therapeutic applications that are discussed in this section. Finally, we provide a future perspective on TALE technology and what challenges remain for successful translation of gene-editing strategies to the clinic.

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Author information

Authors and Affiliations

  1. Stem Cell Program and Institute for Regenerative Cures, University of California, Davis, Sacramento, CA, USA

    Peter Deng, Sakereh Carter & Kyle Fink

  2. Genome Center, MIND Institute, and Biochemistry and Molecular Medicine, University of California, Davis, Davis, CA, USA

    Peter Deng

  3. Department of Neurology, University of California Davis , Sacramento, CA, USA

    Peter Deng, Sakereh Carter & Kyle Fink

Authors
  1. Peter Deng
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  2. Sakereh Carter
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  3. Kyle Fink
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Corresponding author

Correspondence to Kyle Fink .

Editor information

Editors and Affiliations

  1. Translational Science and Molecular Medicine, Michigan State University, Grand Rapids, MI, USA

    Fredric P. Manfredsson

  2. Translational Science and Molecular Medicine, Michigan State University, Grand Rapids, MI, USA

    Matthew J. Benskey

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Deng, P., Carter, S., Fink, K. (2019). Design, Construction, and Application of Transcription Activation-Like Effectors. In: Manfredsson, F., Benskey, M. (eds) Viral Vectors for Gene Therapy. Methods in Molecular Biology, vol 1937. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9065-8_3

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  • DOI: https://doi.org/10.1007/978-1-4939-9065-8_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9064-1

  • Online ISBN: 978-1-4939-9065-8

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