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Phytoplasma Transmission: Insect Rearing and Infection Protocols

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Phytoplasmas

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1875))

Abstract

Phytoplasmas are obligate pathogens and thus they can be studied only in association with their plants or insect hosts. In this chapter, we present protocols for rearing some phytoplasma insect vectors, to obtain infected insects and plants under controlled environmental conditions. We focus on Euscelidius variegatus and Macrosteles quadripunctulatus that can infect Arabidopsis thaliana, and Hyalesthes obsoletus and Scaphoideus titanus, that can infect grapevine.

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Acknowledgment

The authors would like to thank Alberto Loschi (University of Udine, Italy), Filippo Bujan (University of Udine, Italy), and Dr. Stefano Demichelis (University of Torino, Italy) for their help in taking pictures.

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Authors and Affiliations

  1. Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Udine, Italy

    L. Pagliari

  2. IFV, Pôle Nouvelle Aquitaine, Blanquefort, France

    J. Chuche

  3. Department of Agriculture, Forestry and Food Sciences, University of Torino, Grugliasco, Italy

    D. Bosco

  4. INRA, UMR 1065 Save “Santé et Agroécologie du VignoblE”, Villenave d’Ornon, France

    D. Thiéry

  5. UMT Seven “Santé des écosystèmes viticoles économes en intrants”, Villenave d’Ornon, France

    J. Chuche & D. Thiéry

Authors
  1. L. Pagliari
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  2. J. Chuche
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  3. D. Bosco
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  4. D. Thiéry
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Corresponding author

Correspondence to L. Pagliari .

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Editors and Affiliations

  1. Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Udine, Italy

    Rita Musetti

  2. Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Udine, Italy

    Laura Pagliari

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Pagliari, L., Chuche, J., Bosco, D., Thiéry, D. (2019). Phytoplasma Transmission: Insect Rearing and Infection Protocols. In: Musetti, R., Pagliari, L. (eds) Phytoplasmas. Methods in Molecular Biology, vol 1875. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8837-2_3

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  • DOI: https://doi.org/10.1007/978-1-4939-8837-2_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8836-5

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