Abstract
The method described is for early pronuclear transfer (PNT) in normally fertilized human eggs. The PNT procedure should be performed shortly after the appearance of the two pronuclei. Each pronucleus is pinched off with a minimal amount of surrounding cytoplasm to form a membrane enclosed karyoplast. The karyoplasts are then fused with a fertilized egg which has had its pronuclei removed. An experienced individual will achieve approximately 90% survival of the fertilized eggs manipulated.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Schon EA, DiMauro S, Hirano M (2013) Human mitochondrial DNA: roles of inherited and somatic mutations. Nat Rev Genet 13:878–890
Hyslop LA, Blakeley P et al (2016) Towards clinical application of pronuclear transfer to prevent mitochondrial DNA disease. Nature 534(7607):383–386
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Hyslop, L. (2018). Pronuclear Transfer in Human Oocytes. In: Verlhac, MH., Terret, ME. (eds) Mouse Oocyte Development. Methods in Molecular Biology, vol 1818. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8603-3_4
Download citation
DOI: https://doi.org/10.1007/978-1-4939-8603-3_4
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8602-6
Online ISBN: 978-1-4939-8603-3
eBook Packages: Springer Protocols