Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1818)
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About this book
This book details methods on various aspects of the very final stages of mouse oocyte development. Chapters guide the reader through in vitro growth of follicles, production of a fully-grown competent mouse oocyte, meiosis resumption, analysis of spindle assembly and chromosome segregation, analysis of the oocyte and early embryo transcriptome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Mouse Oocyte Development: Methods and Protocols aims to help scientists with the set up both live and fix experiments required to study the process of oocyte meiotic maturation.
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Table of contents (17 protocols)
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Editors and Affiliations
Bibliographic Information
Book Title: Mouse Oocyte Development
Book Subtitle: Methods and Protocols
Editors: Marie-Hélène Verlhac, Marie-Emilie Terret
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-8603-3
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018
Hardcover ISBN: 978-1-4939-8602-6Published: 01 July 2018
Softcover ISBN: 978-1-4939-9340-6Published: 10 December 2019
eBook ISBN: 978-1-4939-8603-3Published: 30 June 2018
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 174
Number of Illustrations: 13 b/w illustrations, 30 illustrations in colour
Topics: Cell Biology