Abstract
Amino acids play an important role in clinical analysis. Capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) has proven to possess several characteristics that make it a powerful and useful tool for the analysis of amino acids in clinical studies. Here we present a method for the separation and quantitative analysis of 27 amino acids in urine based on CE-ESI-MS. The method presents an improved resolution between the isomers Leu, Ile, and aIle, in comparison to other CE-ESI-MS methods in the literature. This method is fast, selective, and simple and has improved sensitivity by applying a pH-mediated stacking strategy, showing that it can be successfully used for amino acid analysis and probably for other small cationic metabolites.
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References
Mayboroda OA, Neusüß C, Pelzing M et al (2007) Amino acid profiling in urine by capillary zone electrophoresis–mass spectrometry. J Chromatogr A 1159:149–153. https://doi.org/10.1016/j.chroma.2007.04.055
Bouatra S, Aziat F, Mandal R et al (2013) The human urine metabolome. PLoS One 8:e73076. https://doi.org/10.1371/journal.pone.0073076
De Benedetto GE (2008) Biomedical applications of amino acid detection by capillary electrophoresis. In: Capillary Electrophor. Humana Press, Totowa, NJ, pp 457–481
Wang C, Zhu H, Pi Z et al (2013) Classification of type 2 diabetes rats based on urine amino acids metabolic profiling by liquid chromatography coupled with tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci 935:26–31. https://doi.org/10.1016/j.jchromb.2013.07.016
Sakaguchi Y, Kinumi T, Yamazaki T, Takatsu A (2015) A novel amino acid analysis method using derivatization of multiple functional groups followed by liquid chromatography/tandem mass spectrometry. Analyst 140:1965–1973. https://doi.org/10.1039/C4AN01672F
Held PK, White L, Pasquali M (2011) Quantitative urine amino acid analysis using liquid chromatography tandem mass spectrometry and aTRAQ reagents. J Chromatogr B Analyt Technol Biomed Life Sci 879:2695–2703. https://doi.org/10.1016/j.jchromb.2011.07.030
Jabeen R, Payne D, Wiktorowicz J et al (2006) Capillary electrophoresis and the clinical laboratory. Electrophoresis 27:2413–2438. https://doi.org/10.1002/elps.200500948
Zhong X, Zhang Z, Jiang S, Li L (2014) Recent advances in coupling capillary electrophoresis-based separation techniques to ESI and MALDI-MS. Electrophoresis 35:1214–1225. https://doi.org/10.1002/elps.201300451
Soga T, Kakazu Y, Robert M et al (2004) Qualitative and quantitative analysis of amino acids by capillary electrophoresis-electrospray ionization-tandem mass spectrometry. Electrophoresis 25:1964–1972. https://doi.org/10.1002/elps.200305791
Soga T, Heiger DN (2000) Amino acid analysis by capillary electrophoresis electrospray ionization mass spectrometry. Anal Chem 72:1236–1241. https://doi.org/10.1021/ac990976y
Ramautar R, Mayboroda OA, Derks RJE et al (2008) Capillary electrophoresis time of flight mass spectrometry using noncovalently bilayer coated capillaries for the analysis of amino acids in human urine. Electrophoresis 29:2714–2722. https://doi.org/10.1002/elps.200700929
Soliman LC, Hui Y, Hewavitharana AK, Chen DDY (2012) Monitoring potential prostate cancer biomarkers in urine by capillary electrophoresis–tandem mass spectrometry. J Chromatogr A 1267:162–169. https://doi.org/10.1016/j.chroma.2012.07.021
Rodrigues KT, Mekahli D, Tavares MFM, Van Schepdael A (2016) Development and validation of a CE-MS method for the targeted assessment of amino acids in urine. Electrophoresis 37:1039–1047. https://doi.org/10.1002/elps.201500534
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Rodrigues, K.T., Tavares, M.F.M., Van Schepdael, A. (2018). CE-MS for the Analysis of Amino Acids. In: Giera, M. (eds) Clinical Metabolomics. Methods in Molecular Biology, vol 1730. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7592-1_23
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DOI: https://doi.org/10.1007/978-1-4939-7592-1_23
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7591-4
Online ISBN: 978-1-4939-7592-1
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