Abstract
Microfluidic systems in combination with microscopy (e.g., fluorescence) can be a powerful tool to study, at single-cell level, the behavior and morphology of biological cells after uptake of glucose. Here, we briefly discuss the advantages of using microfluidic systems. We further describe how microfluidic systems are fabricated and how they are utilized. Finally, we discuss how the large amount of data can be analyzed in a “semi-automatic” manner using custom-made software. In summary, we provide a guide to how to use microfluidic systems in single-cell studies.
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We wish to acknowledge the Science Faculty of the University of Gothenburg for financing and supporting the PDMS Microfluidic Fabrication Facility.
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Welkenhuysen, N., Adiels, C.B., Goksör, M., Hohmann, S. (2018). Applying Microfluidic Systems to Study Effects of Glucose at Single-Cell Level. In: Lindkvist-Petersson, K., Hansen, J. (eds) Glucose Transport. Methods in Molecular Biology, vol 1713. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7507-5_9
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DOI: https://doi.org/10.1007/978-1-4939-7507-5_9
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