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CPMG Experiments for Protein Minor Conformer Structure Determination

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Protein NMR

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1688))

Abstract

CPMG relaxation dispersion NMR experiments have emerged as a powerful method to characterize protein minor states that are in exchange with a visible dominant conformation, and have lifetimes between ~0.5 and 5 milliseconds (ms) and populations greater than 0.5%. The structure of the minor state can, in favorable cases, be determined from the parameters provided by the CPMG relaxation dispersion experiments. Here, we go through the intricacies of setting up these powerful CPMG experiments.

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Acknowledgments

DFH and PV are grateful to Prof. Lewis E Kay (University of Toronto), Dr. Ranjith Muhandiram, Dr. Algirdas Velyvis, Dr. Dmitry Korzhnev, Dr. Vitali Tugarinov, Dr. Philipp Neudecker, Dr. Patrik Lundstrom, and other members of the Kay lab who exposed them to many of the methods presented here. DFH acknowledges the Biotechnology and Biological Sciences Research Council UK (BBSRC) for financial support. PV is supported by generous startup grant from TCIS/TIFH.

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Authors and Affiliations

  1. TIFR Centre for Interdisciplinary Sciences, 21 Brundavan Colony, Narsingi, Hyderabad, 500075, India

    Anusha B. Gopalan & Pramodh Vallurupalli

  2. Division of Biosciences, Institute of Structural and Molecular Biology, University College London, Gower Street, London, WC1E 6BT, UK

    D. Flemming Hansen

Authors
  1. Anusha B. Gopalan
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  2. D. Flemming Hansen
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  3. Pramodh Vallurupalli
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Corresponding authors

Correspondence to Anusha B. Gopalan , D. Flemming Hansen or Pramodh Vallurupalli .

Editor information

Editors and Affiliations

  1. Department of Chemistry and Biochemistry, The City College of New York, New York, New York, USA

    Ranajeet Ghose

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Gopalan, A.B., Hansen, D.F., Vallurupalli, P. (2018). CPMG Experiments for Protein Minor Conformer Structure Determination. In: Ghose, R. (eds) Protein NMR. Methods in Molecular Biology, vol 1688. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7386-6_11

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  • DOI: https://doi.org/10.1007/978-1-4939-7386-6_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7385-9

  • Online ISBN: 978-1-4939-7386-6

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