Abstract
Proton nuclear magnetic resonance spectroscopy (1H NMR) is a useful tool for the analysis of lipid components in biofluids such as serum/plasma. Such tool is applied for the analysis of lipid components in serum/plasma of patients with Duchenne muscular dystrophy (DMD). The practical approach of sampling, storage, lipid extraction procedure, sample preparation before performing the 1H NMR spectroscopy experiments is presented. All the experimental parameters of NMR spectroscopy are also described. Details of the assignments of lipid components (qualitative analysis) and quantification of particular lipid components (quantitative analysis) are explained.
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Abbreviations
- CHOL:
-
Free cholesterol
- CHOLest:
-
Esterified cholesterol
- DMD:
-
Duchenne Muscular Dystrophy
- NMR:
-
Nuclear magnetic resonance
- PL:
-
Phospholipids
- TG:
-
Triglycerides
- TSP:
-
[3-(trimethylsilyl) propionic-2, 2, 3, 3-d4 acid, sodium salt]
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Acknowledgment
The author wishes to thank University Grant Commission, Government of India, for their generous financial support [Grant number: No.F.4-2/2006 (BSR)/13-194/2008 (BSR)].
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Srivastava, N.K. (2018). Proton Nuclear Magnetic Resonance (1H NMR) Spectroscopy-Based Analysis of Lipid Components in Serum/Plasma of Patients with Duchenne Muscular Dystrophy (DMD). In: Bernardini, C. (eds) Duchenne Muscular Dystrophy. Methods in Molecular Biology, vol 1687. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7374-3_14
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DOI: https://doi.org/10.1007/978-1-4939-7374-3_14
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Publisher Name: Humana Press, New York, NY
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