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Purification Method for Recombinant hG-CSF by Affinity Chromatography

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Recombinant Glycoprotein Production

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1674))

Abstract

The human granulocytic colony-stimulating factor (hG-CSF) acts mainly by promoting the maturation of granulocytes and stimulating their phagocytic and chemotactic activity. It has been used in the treatment of many diseases, in particular in neutropenic conditions. Here, we describe the purification process of the recombinant protein hG-CSF expressed in Pichia pastoris. The protein purification proved to be efficient using the nickel affinity chromatography method described in this chapter.

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Acknowledgments

This work was supported by University of São Paulo (USP) and by CAPES.

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Authors and Affiliations

  1. School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil

    Bruna Samham Archangelo & Elisa Maria de Sousa Russo

Authors
  1. Bruna Samham Archangelo
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  2. Elisa Maria de Sousa Russo
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Corresponding author

Correspondence to Elisa Maria de Sousa Russo .

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Editors and Affiliations

  1. Regional Blood Center of Ribeirão Preto, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil

    Virgínia Picanço-Castro

  2. School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil

    Kamilla Swiech

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Archangelo, B.S., de Sousa Russo, E.M. (2018). Purification Method for Recombinant hG-CSF by Affinity Chromatography. In: Picanço-Castro, V., Swiech, K. (eds) Recombinant Glycoprotein Production. Methods in Molecular Biology, vol 1674. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7312-5_16

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  • DOI: https://doi.org/10.1007/978-1-4939-7312-5_16

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7311-8

  • Online ISBN: 978-1-4939-7312-5

  • eBook Packages: Springer Protocols

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