Abstract
The male germline of flowering plants develops within the vegetative cell of the male gametophyte (pollen). The germline is established by asymmetric division of the microspore to form the generative cell. Mitotic division of the generative cell then produces the two sperm cells required for double fertilization. These differentiate to produce the proteins required for gamete attachment and fusion. An important aspect of understanding germline development is the characterization of germline gene expression. Here, we describe the use of a fluorescent reporter to study germline gene expression in developing pollen to assess the timing and specificity of expression.
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Acknowledgments
This work was supported by a Fast Start grant from the Marsden Fund of the Royal Society of New Zealand (12-UOO-043) to L.B. and by research grants from the Biotechnology and Biological Sciences Research Council (BB/I011269/1; BB/N005090/1) to D.T.
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Twell, D., Brownfield, L. (2017). Analysis of Fluorescent Reporter Activity in the Male Germline During Pollen Development by Confocal Microscopy. In: Schmidt, A. (eds) Plant Germline Development. Methods in Molecular Biology, vol 1669. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7286-9_6
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DOI: https://doi.org/10.1007/978-1-4939-7286-9_6
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7286-9
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