Abstract
Circulating tumor cells (CTCs) are an important biomarker and their analysis can be considered a form of “liquid biopsy.” The purpose of this book chapter is to describe the use of the 4-channel CMx (cells captured in maximum) microfluidic chip, containing special micropatterns coated with an antibody-conjugated supported lipid bilayer (SLB) on its surface, to capture and isolate CTCs from the blood of cancer patients. Captured CTCs are subsequently released by an air foam to an immunofluorescence (IF) staining panel that enables further analysis, including the identification of the primary cancer source of the CTCs.
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Acknowledgment
This work was supported by Genomics Research Center, Academia Sinica, Taiwan and Ministry of Science and Technology of Taiwan under contract 105-0210-01-13-01, MOST-104-2113-M-001-015-MY3, MOST 105-2113-M-037-MY2, AS-105-TP-A04. We thank Katherine Yih Ruey Chen for proof reading.
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Lai, CH., Chang, YC. (2017). Microfluidic Capture and Multiplex Immunofluorescence of Circulating Tumor Cells to Identify Cancer of Origin. In: M. Magbanua, M., W. Park, J. (eds) Circulating Tumor Cells. Methods in Molecular Biology, vol 1634. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7144-2_1
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DOI: https://doi.org/10.1007/978-1-4939-7144-2_1
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