Abstract
IL-9-producing T helper cells (Th9) have recently emerged as an important T cell subset contributing to the pathogenicity of allergic diseases. Here, we describe the role of Th9 cells in allergic inflammation and provide detailed protocols to characterize IL-9-producing T cells and analyze OVA-specific IL-9 production in allergic lung diseases using a mouse model of OVA-induced chronic allergic lung inflammation.
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Acknowledgment
We gratefully thank all members of the Angkasekwinai lab and the Chen Dong lab at the University of Texas MD Anderson Cancer Center for optimization of this protocol and for lab training.
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Angkasekwinai, P. (2017). Allergic Inflammation and Atopic Disease: Role of Th9 Cells. In: Goswami, R. (eds) Th9 Cells. Methods in Molecular Biology, vol 1585. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6877-0_15
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DOI: https://doi.org/10.1007/978-1-4939-6877-0_15
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