Abstract
Fourier transform infrared (FTIR) spectroscopy is widely used in the pharmaceutical industry for process monitoring, compositional quantification, and characterization of critical quality attributes in complex mixtures. Advantages over other spectroscopic measurements include ease of sample preparation, quantification of multiple components from a single measurement, and the ability to quantify optically opaque samples. This method describes the use of a multivariate model for quantifying a TLR4 agonist (GLA) adsorbed onto aluminum oxyhydroxide (Alhydrogel®) using FTIR spectroscopy that may be adapted to quantify other complex aluminum based adjuvant mixtures.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Hem SL, HogenEsch H (2007) Aluminum-containing adjuvants: properties, formulation, and use. In: Singh M (ed) Vaccine adjuvants and delivery systems. John Wiley & Sons, Hoboken, NJ, pp 81–114
Anderson RC, Fox CB, Dutill TS, Shaverdian N, Evers TL, Poshusta GR, Chesko J, Coler RN, Friede M, Reed SG, Vedvick TS (2010) Physicochemical characterization and biological activity of synthetic TLR4 agonist formulations. Colloids Surf B: Biointerfaces 75(1):123–132
Kensil CR, Patel U, Lennick M, Marciani D (1991) Separation and characterization of saponins with adjuvant activity from Quillaja saponaria Molina cortex. J Immunol 146(2):431–437
Salari A, Young RE (1998) Application of attenuated total reflectance FTIR spectroscopy to the analysis of mixtures of pharmaceutical polymorphs. Int J Pharm 163(1–2):157–166
Van Eerdenbrugh B, Taylor LS (2011) Application of mid-IR spectroscopy for the characterization of pharmaceutical systems. Int J Pharm 417(1–2):3–16
Agopian A, Ronzon F, Sauzeat E, Sodoyer R, El Habib R, Buchet R, Chevalier M (2007) Secondary structure analysis of HIV-1-gp41 in solution and adsorbed to aluminum hydroxide by Fourier transform infrared spectroscopy. Biochim Biophys Acta 1774(3):351–358
Dowling QM, Schwartz AM, Vedvick TS, Fox CB, Kramer RM (2014) Quantitative measurement of toll-like receptor 4 agonists adsorbed to alhydrogel by fourier transform infrared-attenuated total reflectance spectroscopy. J Pharm Sci 104(2):768–74
Johnston CT, Wang SL, Hem SL (2002) Measuring the surface area of aluminum hydroxide adjuvant. J Pharm Sci 91(7):1702–1706
Rinella JV Jr, White JL, Hem SL (1995) Effect of anions on model aluminum-adjuvant-containing vaccines. J Colloid Interface Sci 172(1):121–130
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media New York
About this protocol
Cite this protocol
Dowling, Q.M., Kramer, R.M. (2017). Quantification of Multiple Components of Complex Aluminum-Based Adjuvant Mixtures by Using Fourier Transform Infrared Spectroscopy and Partial Least Squares Modeling. In: Fox, C. (eds) Vaccine Adjuvants. Methods in Molecular Biology, vol 1494. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6445-1_18
Download citation
DOI: https://doi.org/10.1007/978-1-4939-6445-1_18
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6443-7
Online ISBN: 978-1-4939-6445-1
eBook Packages: Springer Protocols