Abstract
Allogeneic islet transplantation has become a viable option for the treatment of unstable type 1 diabetes; however, donor shortage and the necessity for immunosuppressive drugs are two major drawbacks of this treatment. Microencapsulated porcine islets can solve both of these drawbacks, and clinical trials have been conducted. Previous clinical trials demonstrated the safety and marginal efficacy of this treatment; therefore, it is necessary to improve its efficacy. The production of microencapsulated porcine islets consists of pancreas procurement, islet isolation, and microencapsulation. In this chapter, we describe our current methods for porcine pancreas procurement, islet isolation, and microencapsulation.
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References
Bartlett ST, Markmann JF, Johnson P, et al (2015) Report from IPITA-TTS opinion leaders meeting on the future of beta cell replacement. Transplantation (in press)
Bruni A, Gala-Lopez B, Pepper AR et al (2014) Islet cell transplantation for the treatment of type I diabetes: recent advances and future challenges. Diabetes Metab Syndr Obes 7:211–223
Matsumoto S (2010) Islet cell transplantation for type 1 diabetes. J Diabetes 2:16–22
Elliott RB, Escobar L, Tan PLJ et al (2007) Live encapsulated porcine islets from a type 1 diabetic patient 9.5 yr after xenotransplantation. Xenotransplantation 14:157–161
Korbutt GS, Elliott JF, Ao Z et al (1996) Large scale isolation, growth and function of porcine neonatal islet cells. J Clin Invest 97:270–280
Calafiore R, Basta G (2014) Clinical application of microencapsulated islets: actual prospective on progress and challenges. Adv Drug Deliv Rev 67–68:84–92
Matsumoto S, Tan P, Baker J et al (2014) Clinical porcine islet xenotransplantation under comprehensive regulation. Transplant Proc 46:1992–1995
Matsumoto S, Takita M, Chaussabel D et al (2011) Improving efficacy of clinical islet transplantation with iodixanol-based islet purification, thymoglobulin induction, and blockage of IL-1beta and TNF-alpha. Cell Transplant 20:1641–1647
Matsumoto S, Noguchi H, Shimoda M et al (2010) Seven consecutive successful clinical islet isolations with pancreatic ductal injection. Cell Transplant 19:291–297
Shimoda M, Noguchi H, Fujita Y et al (2012) Improvement of collagenase distribution with the ductal preservation for human islet isolation. Islets 4:130–137
Qin H, Matsumoto S, Klintmalm GB et al (2011) A meta-analysis for comparison of the two-layer and university of Wisconsin pancreas preservation methods in islet isolation. Cell Transplant 20:1127–1137
Calafiore R, Basta G, Luca G et al (2006) Standard technical procedures for microencapsulation of human islets for graft into nonimmunosuppressed patients with type 1 diabetes mellitus. Transplant Proc 38:1156–1157
Hillberg A, Kathirgamanathan K, Lam JBB et al (2013) Improving alginate-poly-L-ornithine-alginate capsule biocompatibility through genipin crosslinking. J Biomed Mater Res B Appl Biomater 101:258–268
Kitzmann JP, Law L, Shome A et al (2012) Real-time assessment of encapsulated neonatal porcine islets prior to clinical xenotransplantation. Xenotransplantation 19:333–336
Matsumoto S, Qualley SA, Goel S et al (2002) Effect of the two-layer (University of Wisconsin solution-perfluorochemical plus O2) method of pancreas preservation on human islet isolation, as assessed by the Edmonton isolation protocol. Transplantation 2002(74):1414–1419
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Shimoda, M., Matsumoto, S. (2017). Methods for Microencapsulated Porcine Islet Production. In: Opara, E. (eds) Cell Microencapsulation. Methods in Molecular Biology, vol 1479. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6364-5_26
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DOI: https://doi.org/10.1007/978-1-4939-6364-5_26
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-6364-5
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