Abstract
Islet transplantation is the most exciting treatment option for individuals afflicted with Type 1 diabetes. However, the severe shortage of human pancreas and the need to use risky immunosuppressive drugs to prevent transplant rejection remain two major obstacles for the routine use of islet transplantation in diabetic patients. Successful development of a bioartificial pancreas using the approach of microencapsulation with perm-selective coating of islets with biopolymers for graft immunoisolation holds tremendous promise for diabetic patients because it has great potential to overcome these two barriers. In this chapter, we provide a detailed description of the microencapsulation process.
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References
Pareta RA, McQuilling JP, Farney A, Opara EC (2012) Bioartificial pancreas: evaluation of crucial barriers to clinical application. In: Organ donation, chapter 14. INTECH Publishers, pp 241–266. ISBN 979-953-307-081-9
Soon-Shiong P, Feldman E, Nelson R et al (1992) Successful reversal of spontaneous diabetes in dogs by intraperitoneal microencapsulated islets. Transplantation 54:769–774
Calafiore R, Calabrese G, Basta G et al (2006) Microencapsulated pancreatic islet allograft into non-immunosuppressed patients with Type 1 diabetes. Diabetes Care 29(1):137–138
Elliott RB, Escobar L, Tan PLJ et al (2007) Live encapsulated porcine islets from type 1 diabetic patient 9.5 yr after xenotransplantation. Xenotransplantation 14:157–161
http://www.newscientist.com/article/dn18730-pig-sushi-diabetes-trial-brings-xenotransplant-hope.html
Smidsrod O, Skjak-Braek G (1990) Alginate as immobilization matrix for cells. Trends Biotechnol 8:71–78
Moya ML, Morley M, Khanna O et al (2012) Stability of alginate microbead properties in vitro. J Mater Sci Mater Med 23:903–912
Wolters GH, Fritschy WM, Gerrits D et al (1991) A versatile alginate droplet generator applicable for microencapsulation of pancreatic islets. J Appl Biomater 3:281–286
Hongkwan P, Kim PH, Hwang T et al (2012) Fabrication of cross-linked alginate beads using electrospraying for adenovirus delivery. Int J Pharm 427:417–425
Garfinkel MR, Harland RC, Opara EC (1998) Optimization of the microencapsulated islet for transplantation. J Surg Res 76:7–10
Zimmermann U, Mimietz S, Zimmermann H et al (2000) Hydrogel-based non-autologous cell and tissue therapy. Biotechniques 29:564–581
Darrabie MD, Kendall WF, Opara EC (2005) Characteristics of poly-L-ornithine-coated alginate microcapsules. Biomaterials 26(34):6846–6852
Opara EC, Mirmalek-Sani S-H, Khanna O et al (2010) Design of a bioartificial pancreas. J Investig Med 58(7):831–837
Khanna O, Moya ML, Opara EC et al (2010) Synthesis of multi-layered alginate microcapsules for the sustained release of fibroblast growth factor-1. J Biomed Mater Res A 95(2):632–640
Tendulkar S, Mirmalek-Sani S-H, Childers C et al (2012) A three-dimensional microfluidic approach to scaling up microencapsulation of cells. Biomed Microdevices 14(3):461–469
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Opara, E.C., McQuilling, J.P., Farney, A.C. (2013). Microencapsulation of Pancreatic Islets for Use in a Bioartificial Pancreas. In: Basu, J., Ludlow, J. (eds) Organ Regeneration. Methods in Molecular Biology, vol 1001. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-363-3_21
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DOI: https://doi.org/10.1007/978-1-62703-363-3_21
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Publisher Name: Humana Press, Totowa, NJ
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